FlyBase Allele Report: Dmel\Pisd[NIG.5991R]

General Information

Symbol

Dmel\Pisd^NIG.5991R

Species

D. melanogaster

Name

FlyBase ID

FBal0274931

Feature type

allele

Associated gene

Dmel\Pisd

Associated Insertion(s)

Carried in Construct

P{NIG.5991R}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Japanese National Institute of Genetics, 2012.5.21)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Japanese National Institute of Genetics, 2012.5.21)

Mutagen

in vitro construct

(Japanese National Institute of Genetics, 2012.5.21)

Progenitor genotype

Carried in construct

P{NIG.5991R}

Cytology

Description

UASt regulatory sequences drive expression of a 500bp inverted repeat.

(Japanese National Institute of Genetics, 2012.5.21)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-5991R

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of retinal disease

CEA

(Midorikawa et al., 2010)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates retinal disease

modeled by bbc^NIG.6016R

(Midorikawa et al., 2010)

model of retinal disease

is ameliorated by Rab7^Tub.Venus

(Midorikawa et al., 2010)

is exacerbated by bbc^NIG.6016R

(Midorikawa et al., 2010)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death | adult stage | light conditional, with Scer\GAL4^GMR.PU

(Midorikawa et al., 2010)

Phenotype Manifest In

autolysosome | adult stage | light conditional, with Scer\GAL4^GMR.PU

(Midorikawa et al., 2010)

autophagosome | adult stage | light conditional, with Scer\GAL4^GMR.PU

(Midorikawa et al., 2010)

ommatidium | adult stage | light conditional, with Scer\GAL4^GMR.PU

(Midorikawa et al., 2010)

retina | adult stage | light conditional, with Scer\GAL4^GMR.PU

(Midorikawa et al., 2010)

rhabdomere | adult stage | decreased number | light conditional, with Scer\GAL4^GMR.PU

(Midorikawa et al., 2010)

Detailed Description

Statement

Reference

Expressing Pisd^NIG.5991R under the control of Scer\GAL4^GMR.PU induces light-dependent ommatidial degeneration (ommatidium disruption and rhabdomere loss), associated with retinal apoptosis. Retinal cells fail to form large autophagosomes/autolysosomes in response to light exposure. Ommatidial degeneration occurs in adults reared under constant light for 5d or under blue light for 10min followed by 5d of darkness.

(Midorikawa et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Pisd^NIG.5991R, Scer\GAL4^GMR.PU has ommatidium | adult stage | light conditional phenotype, enhanceable by bbc^NIG.6016R, Scer\GAL4^GMR.PU

(Midorikawa et al., 2010)