FB2024_03 , released June 25, 2024
Allele: Dmel\me31BNIG.4916R
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General Information
Symbol
Dmel\me31BNIG.4916R
Species
D. melanogaster
Name
FlyBase ID
FBal0274435
Feature type
allele
Associated gene
Dmel\me31B
Associated Insertion(s)
Carried in Construct
P{NIG.4916R}
Key Links
Genomic Maps

Transgenic product class
RNAi_reagent
(Japanese National Institute of Genetics, 2012.5.21)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Japanese National Institute of Genetics, 2012.5.21)
Mutagen
in vitro construct
(Japanese National Institute of Genetics, 2012.5.21)
Progenitor genotype
Carried in construct
P{NIG.4916R}
Cytology
Description

UASt regulatory sequences drive expression of a 500bp inverted repeat.

(Japanese National Institute of Genetics, 2012.5.21)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
dsRNA-4916R
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of me31BNIG.4916R (together with UAS-Dicer2 to enhance RNAi efficiency) under the control of Scer\GAL4tim.PE results in poor rhythmicity of adult circadian locomotor activity under free-running (~constant darkness, DD) conditions and while the daily oscillation of per and tim proteins (under standard light-dark conditions) are not perturbed, the oscillation of the Pdf protein in the axons of s-LNv neurons is abolished.

      Poor locomotor behavior rhythmicity in DD conditions is also observed when the me31BNIG.4916R is driven by either Scer\GAL4P2.4.Pdf or Scer\GAL4cry.PE.

      (Lee et al., 2017)

      Expression of me31BNIG.4916R under the control of Scer\GAL4GH146 completely blocks the long-term habituation (LTH) seen when flies are exposed to ethyl butyrate (EB) for 4 days.

      (McCann et al., 2011)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      NOT Enhancer of
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The poor rhythmicity of adult circadian locomotor activity under free-running (~constant darkness) conditions observed in flies expressing me31BNIG.4916R under the control of Scer\GAL4cry.PE is further worsened by co-expression of Atx2KK100423.

      The aberrant long-period circadian locomotor rhythm under free-running constant dark conditions characteristic for adult flies expressing tyfΔC5.Scer\UAS.T:SV5\V5 under the control of Scer\GAL4Mz520 is not enhanced by co-expression of me31BNIG.4916R.

      (Lee et al., 2017)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      me31BNIG.4916R
      Name Synonyms
      Secondary FlyBase IDs
        References (4)