FB2024_03 , released June 25, 2024
Allele: Dmel\Sec20NIG.2023R
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General Information
Symbol
Dmel\Sec20NIG.2023R
Species
D. melanogaster
Name
FlyBase ID
FBal0273009
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Nature of the Allele
Progenitor genotype
Carried in construct
Cytology
Description

UASt regulatory sequences drive expression of a 500bp inverted repeat.

Allele components
Component
Use(s)
Encoded product / tool
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DNA sequence
Protein sequence
 
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expressing Sec20NIG.2023R under the control of Scer\GAL4pros-V1 leads to larval garland nephrocytes showing endocytosis/lysosomal/autophagy defects, as there are enlarged Rab7-positive late endosomes, although Rbsn-5-positive early endosomes have similar size and distribution as controls.

Clonal expression of Sec20NIG.2023R under the control of Scer\GAL4Act5C.PP (in combination with Dicer-2, for a more efficient RNAi) leads to larval fat cells showing autophagy defects: increase in abundancy but decrease in size of autophagic vesicles (Atg8 puncta in fluorescence microscopy); and decreased autophagy flux (using mCherry-GFP-Atg8a fluorescence reporter).

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
CG2023NIG.2023R
Sec20NIG.2023R
Name Synonyms
Secondary FlyBase IDs
    References (2)