FlyBase Allele Report: Dmel\Klp64D[NIG.10642R]

General Information

Symbol

Dmel\Klp64D^NIG.10642R

Species

D. melanogaster

Name

FlyBase ID

FBal0270549

Feature type

allele

Associated gene

Dmel\Klp64D

Associated Insertion(s)

Carried in Construct

P{NIG.10642R}

Also Known As

UAS-klp64D RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Japanese National Institute of Genetics, 2012.5.21)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Japanese National Institute of Genetics, 2012.5.21)

Mutagen

in vitro construct

(Japanese National Institute of Genetics, 2012.5.21)

Progenitor genotype

Carried in construct

P{NIG.10642R}

Cytology

Description

UASt regulatory sequences drive expression of a 500bp inverted repeat.

(Japanese National Institute of Genetics, 2012.5.21)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-10642R

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of P-stage | heat sensitive, with Scer\GAL4^nub-AC-62

(Vuong et al., 2014)

visible, with Scer\GAL4^bbg-C96

(Vuong et al., 2014)

visible, with Scer\GAL4^nub-AC-62

(Vuong et al., 2014)

Phenotype Manifest In

wing, with Scer\GAL4^nub-AC-62

(Vuong et al., 2014)

wing margin, with Scer\GAL4^bbg-C96

(Vuong et al., 2014)

wing margin, with Scer\GAL4^nub-AC-62

(Vuong et al., 2014)

wing margin bristle, with Scer\GAL4^nub-AC-62

(Vuong et al., 2014)

Detailed Description

Statement

Reference

At 18[o]C, nearly all animals expressing Klp64D^NIG.10642R under the control of Scer\GAL4^nub-AC-62 show mild defects in the distal part of the wings. Most of the defects seen comprise notching in the wing margin and loss of margin bristles. Phenotypes are temperature-dependent: at 29[o]C, flies can almost develop to the adult but die in the pupal case. These flies show severe shrinkage or complete loss of wing.

Expressing Klp64D^NIG.10642R at and near the DV boundary region using Scer\GAL4^bbg-C96 causes notching along the wing margin. This phenotype is seen with 100% penetrance.

(Vuong et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Klp64D^NIG.10642R, Scer\GAL4^bbg-C96 has visible phenotype, suppressible by arm^{S10.UAS.Tag:MYC}, Scer\GAL4^bbg-C96

(Vuong et al., 2014)

Klp64D^NIG.10642R, Scer\GAL4^bbg-C96 has visible phenotype, suppressible by Hsap\KIF3A^UAS.cGa, Scer\GAL4^bbg-C96

(Vuong et al., 2014)

NOT suppressed by

Statement

Reference

Klp64D^NIG.10642R, Scer\GAL4^bbg-C96 has visible phenotype, non-suppressible by wg^UAS.GFP, Scer\GAL4^bbg-C96

(Vuong et al., 2014)

Klp64D^NIG.10642R, Scer\GAL4^bbg-C96 has visible phenotype, non-suppressible by arm^{S2.UAS.Tag:MYC}, Scer\GAL4^bbg-C96

(Vuong et al., 2014)

Klp64D^NIG.10642R, Scer\GAL4^bbg-C96 has visible phenotype, non-suppressible by arm^UAS.Exel, Scer\GAL4^bbg-C96

(Vuong et al., 2014)

Klp64D^NIG.10642R, Scer\GAL4^bbg-C96 has visible phenotype, non-suppressible by arm¹

(Vuong et al., 2014)

Suppressor of

Statement

Reference

Klp64D^NIG.10642R, Scer\GAL4^bbg-C96 is a suppressor | partially of lethal - all die before end of P-stage | heat sensitive phenotype of Scer\GAL4^bbg-C96, wg^UAS.GFP

(Vuong et al., 2014)

Other

Statement

Reference

Klp64D^NIG.10642R, Scer\GAL4^bbg-C96, wg^UAS.GFP has visible phenotype

(Vuong et al., 2014)

Phenotype Manifest In

Suppressed by

Statement

Reference

Klp64D^NIG.10642R, Scer\GAL4^bbg-C96 has wing margin phenotype, suppressible by arm^{S10.UAS.Tag:MYC}, Scer\GAL4^bbg-C96

(Vuong et al., 2014)

Klp64D^NIG.10642R, Scer\GAL4^bbg-C96 has wing margin phenotype, suppressible by Hsap\KIF3A^UAS.cGa, Scer\GAL4^bbg-C96

(Vuong et al., 2014)

NOT suppressed by

Statement

Reference

Klp64D^NIG.10642R, Scer\GAL4^bbg-C96 has wing margin phenotype, non-suppressible by wg^UAS.GFP, Scer\GAL4^bbg-C96

(Vuong et al., 2014)

Klp64D^NIG.10642R, Scer\GAL4^bbg-C96 has wing margin phenotype, non-suppressible by arm^{S2.UAS.Tag:MYC}, Scer\GAL4^bbg-C96

(Vuong et al., 2014)

Klp64D^NIG.10642R, Scer\GAL4^bbg-C96 has wing margin phenotype, non-suppressible by arm^UAS.Exel, Scer\GAL4^bbg-C96

(Vuong et al., 2014)

Klp64D^NIG.10642R, Scer\GAL4^bbg-C96 has wing margin phenotype, non-suppressible by arm¹

(Vuong et al., 2014)

Other

Statement

Reference

Klp64D^NIG.10642R, Scer\GAL4^bbg-C96, wg^UAS.GFP has wing phenotype

(Vuong et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of wg^{Scer\UAS.T:Avic\GFP} does not suppress the wing notching seen when Klp64D^NIG.10642R is expressed under the control of Scer\GAL4^bbg-C96.

Expression of Klp64D^NIG.10642R partially rescues the lethality seen when wg^{Scer\UAS.T:Avic\GFP} is expressed under the control of Scer\GAL4^bbg-C96 at 25[o]C, resulting in the survival of 30% of adult flies with small wings.

Expression of dsh^{Scer\UAS.T:Hsap\MYC} does not suppress the wing notching seen when Klp64D^NIG.10642R is expressed under the control of Scer\GAL4^bbg-C96.

Expression of arm^{S10.Scer\UAS.T:Hsap\MYC} strongly suppresses the wing notching seen when Klp64D^NIG.10642R is expressed under the control of Scer\GAL4^bbg-C96. The wing margin bristles are partially restored.

Expression of arm^{S2.Scer\UAS.T:Hsap\MYC} does not suppress the wing notching seen when Klp64D^NIG.10642R is expressed under the control of Scer\GAL4^bbg-C96.

Expression of arm^{Scer\UAS.Exel} strongly suppresses the wing notching seen when Klp64D^NIG.10642R is expressed under the control of Scer\GAL4^bbg-C96. The wing margin bristles are partially restored.

Heterozygous arm¹ enhances the wing notching and wing hair loss seen when Klp64D^NIG.10642R is expressed under the control of Scer\GAL4^nub-AC-62.

(Vuong et al., 2014)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\KIF3A^Scer\UAS.cGa strongly fully suppresses the wing notching seen when Klp64D^NIG.10642R is expressed under the control of Scer\GAL4^bbg-C96.

(Vuong et al., 2014)

Complementation and Rescue Data

Rescued by

Klp64D^NIG.10642R is rescued by Scer\GAL4^Act.PU/Klp64D^UAS.Tag:HA

(Vuong et al., 2014)

Not rescued by

Klp64D^NIG.10642R is not rescued by Klp64D^T114A.UAS/Scer\GAL4^bbg-C96

(Vuong et al., 2014)

Klp64D^NIG.10642R is not rescued by Scer\GAL4^bbg-C96/Klp64D^ΔABD.UAS

(Vuong et al., 2014)

Comments

Expression of Klp64D^{Scer\UAS.T:Ivir\HA1} rescues the wing phenotype caused by expression of Klp64D^NIG.10642R under the control of Scer\GAL4^Act.PU.

Expression of Klp64D^{T114A.Scer\UAS} is unable to rescue the wing phenotype seen when Klp64D^NIG.10642R is expressed under the control of Scer\GAL4^bbg-C96.

Expression of Klp64D^{ΔABD.Scer\UAS} is unable to rescue the wing phenotype seen when Klp64D^NIG.10642R is expressed under the control of Scer\GAL4^bbg-C96.

(Vuong et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Klp64D^NIG.10642R

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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