FB2024_03 , released June 25, 2024
Allele: Dmel\shits1.IVS.Syn21.p10.20xUAS
Open Close
General Information
Symbol
Dmel\shits1.IVS.Syn21.p10.20xUAS
Species
D. melanogaster
Name
FlyBase ID
FBal0270223
Feature type
allele
Associated gene
Dmel\shi
Associated Insertion(s)
Carried in Construct
P{20XUAS-IVS-Syn21-shits1-p10}
PBac{20XUAS-IVS-Syn21-shits1-p10}
Also Known As
UAS-shibirets, pJFRC99-20XUAS-IVS-Syn21-Shibire-ts1-p10
Key Links
Transgenic product class
dominant_negative_variant
(Pfeiffer et al., 2012)
missense_variant
(Pfeiffer et al., 2012)
Mutagen
Nature of the Allele
Transgenic product class
dominant_negative_variant
(Pfeiffer et al., 2012)
missense_variant
(Pfeiffer et al., 2012)
Mutagen
in vitro construct
(Pfeiffer et al., 2012)
Progenitor genotype
Carried in construct
P{20XUAS-IVS-Syn21-shits1-p10}
PBac{20XUAS-IVS-Syn21-shits1-p10}
Cytology
Description

20 copies of the UAS site are fused upstream of the shi1 coding region (this encodes a temperature-sensitive dominant negative form of shi). A small intron derived from the Mhc gene followed by a synthetic AT-rich 21bp sequence made by combining the Cavener consensus sequence with elements from the Malacosoma neustria nucleopolyhedrovirus (MnNPV) polyhedrin gene has been inserted between the promoter and coding sequences. The 3' UTR (666bp) from the Autographa californica nucleopolyhedrovirus (AcNPV) p10 gene is present downstream of the coding sequence.

(Pfeiffer et al., 2012)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
shi
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Mushroom body gamma neurons expressing shits1.IVS.Syn21.p10.20xScer\UAS under the control of Scer\GAL4Tab2-201Y show an axon pruning defect at the restrictive temperature (but not at the permissive temperature).

      (Issman-Zecharya and Schuldiner, 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Shits1-pJFRC99
      (Issman-Zecharya and Schuldiner, 2014)
      shits1.IVS.Syn21.p10.20xScer\UAS
      shits1.IVS.Syn21.p10.20xUAS
      Name Synonyms
      Secondary FlyBase IDs
        References (9)