FB2024_03 , released June 25, 2024
Allele: Dmel\Egfr::HsapEGFRhE-E.UAS.GFP
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General Information
Symbol
Dmel\Egfr::Hsap\EGFRhE-E.UAS.GFP
Species
D. melanogaster
Name
FlyBase ID
FBal0268318
Feature type
allele
Associated gene
Egfr::Hsap\EGFR
Associated Insertion(s)
Carried in Construct
M{UAS-hE-Egfr-GFP}
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Inaki et al., 2012)
Progenitor genotype
Carried in construct
M{UAS-hE-Egfr-GFP}
Cytology
Description

UAS regulatory sequences drive expression of the extracellular and transmembrane domains of Hsap\EGFR fused to the intracellular domain of Egfr. The protein is tagged at the C-terminal end with GFP.

(Inaki et al., 2012)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Egfr::Hsap\EGFR
Tagged with
GFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      Co-expression of Hsap\TGFAScer\UAS.T:Ivir\HA1 and Egfr::Hsap\EGFRhE-E.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4slbo.2.6 results in strong defects in border cell migration, with no migration being seen in over 70% of stage 10 egg chambers.

      The effect on border cell cluster migration when all border cells co-express PvrDN.Scer\UAS and EgfrDN.Scer\UAS under the control of Scer\GAL4slbo.2.6 and a single border cell expresses Egfr::Hsap\EGFRhE-E.Scer\UAS.T:Avic\GFP have been studied. In those border cell clusters where the single Egfr::Hsap\EGFRhE-E.Scer\UAS.T:Avic\GFP-expressing cell is located at the front of the cluster, the cluster on average moves forward, when the Egfr::Hsap\EGFRhE-E.Scer\UAS.T:Avic\GFP-expressing cell is in the back, the cluster on average moves relatively more backwards.

      (Inaki et al., 2012)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Egfr::Hsap\EGFRhE-E.Scer\UAS.T:Avic\GFP
      Egfr::Hsap\EGFRhE-E.UAS.GFP
      Name Synonyms
      Secondary FlyBase IDs
        References (3)