Imprecise excision of the progenitor insertion, resulting in a 16kb deletion that covers the AGO1 coding sequence.
Homozygous female germline clones result in variable loss of the oocyte. The frequency of egg chambers lacking an oocyte is dramatically increased at 14 days after eclosion (DAE) compared to 7 DAE. At 14 DAE, the most commonly observed developmental arrest amongst egg chambers lacking an oocyte is at 8 nurse cells.
AGO1k08121/AGO114 females in which expression of AGO1hs.PK via daily heat shock has been used to provide AGO1 function until eclosion have normal ovaries 3 days after eclosion. However, at 15 days old these flies show strong defects in germline stem cell maintenance in the germaria: only 10.6% of germaria have a normal looking structure containing two stem cells, 23.9% contain a single stem cell, 60.1% contain differentiated germ cells with branched fusomes only and 5.3% contain no germ cells at all.
AGO1k08121/AGO114 germline stem cell clones are lost from the ovary at a faster rate than marked control clones.
