FB2024_03 , released June 25, 2024
Allele: Dmel\AGO1EMS
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General Information
Symbol
Dmel\AGO1EMS
Species
D. melanogaster
Name
FlyBase ID
FBal0268165
Feature type
allele
Associated gene
Dmel\AGO1
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Yang et al., 2007)
Progenitor genotype
Cytology
Description

C to T nucleotide substitution which changes amino acid Q212 to a stop codon in the B isoform and changes amino acid Q246 to a stop codon in the A and C isoforms.

(Yang et al., 2007)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2R:13,950,378..13,950,378 [-]
Nucleotide change:

C13950378T

Reported nucleotide change:

C?T

Amino acid change:

Q246term | AGO1-PA; Q212term | AGO1-PB; Q246term | AGO1-PC; Q246term | AGO1-PD

Reported amino acid change:

Q246term

Comment:

Reported as Q246 in isoforms AGO1-RA and AGO1-RC and Q212 in isoform AGO1-RB.

(Yang et al., 2007)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Homozygous female germline clones result in variable loss of the oocyte. 66% of egg chambers lack an oocyte at 7 days after eclosion. The most commonly observed developmental arrest amongst egg chambers lacking an oocyte is at 8 nurse cells.

      (Azzam et al., 2012)

      AGO1k08121/AGO1EMS females in which expression of AGO1hs.PK via daily heat shock has been used to provide AGO1 function until eclosion have normal ovaries 3 days after eclosion. However, at 15 days old these flies show strong defects in germline stem cell maintenance in the germaria: only 10.3% of germaria have a normal looking structure containing two stem cells, 26.5% contain a single stem cell, 60.3% contain differentiated germ cells with branched fusomes only and 2.9% contain no germ cells at all.

      AGO1k08121/AGO1EMS germline stem cell clones are lost from the ovary at a faster rate than marked control clones.

      (Yang et al., 2007)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      AGO1EMS
      Ago1EMS
      (Pinder and Smibert, 2013, Yang et al., 2007)
      ago1EMS
      (Azzam et al., 2012)
      Name Synonyms
      Secondary FlyBase IDs
        References (3)