FB2024_03 , released June 25, 2024
Allele: Dmel\P32EC1
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General Information
Symbol
Dmel\P32EC1
Species
D. melanogaster
Name
FlyBase ID
FBal0267752
Feature type
allele
Associated gene
Dmel\P32
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

loss of function allele

Mutagen
Nature of the Allele
Allele class

loss of function allele

(Lutas et al., 2012)
Mutagen
ethyl methanesulfonate
(Lutas et al., 2012)
Progenitor genotype
Cytology
Description

A single nucleotide deletion in the P32 ORF resulting in a premature stop codon that truncates the protein after 101 amino acids. "C" at position 303 is deleted, and thus the resulting codon change is from "GCC GTA A" to "GCG TAA".

(Lutas et al., 2012)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
2R:17,672,112..17,672,112 [-]
Reported amino acid change:

V102term

Comment:

A single nucleotide deletion in the P32 ORF resulting in a premature stop codon that truncates the protein after 101 amino acids. "C" at position 303 is deleted, and thus the resulting codon change is from "GCC GTA A" to "GCG TAA".

(Lutas et al., 2012)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      P32EC1/P32EC1 or P32EC1/Df(2R)14F06W-07 flies are paralysed rapidly at 38[o]C.

      P32EC1 flies show a heat-sensitive reduction in the excitatory postsynaptic current amplitude.

      DLM neuromuscular synapses of P32EC1 flies at the restrictive temperature show proper transport and localization of pre-synaptic mitochondria.

      At both 20[o]C and the restrictive temperature of 36[o]C, P32EC1 flies exhibit a wild type mitochondrial membrane potential.

      ATP measurements on head homogenates from P32EC1 flies exposed to the restrictive temperature of 38[o]C indicate no significant difference from controls.

      At the restrictive temperature, the presynaptic calcium transients elicited by 40 Hz stimulation is increased in the DLM neuromuscular synapses of P32EC1/Df(2R)14F06W-07 flies compared to controls. Resting presynaptic calcium is also increased in the mutant flies at elevated temperatures.

      (Lutas et al., 2012)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhancer of
      Statement
      Reference

      P32EC1/P32EC1 is an enhancer of paralytic | heat sensitive phenotype of cacTS2

      (Lutas et al., 2012)

      P32EC1 is an enhancer of abnormal neurophysiology | heat sensitive phenotype of cacTS2

      (Lutas et al., 2012)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Unlike cacTS2 alone, cacTS2 P32EC1 double mutants exhibit rapid paralysis at 36[o]C.

      P32EC1 strongly enhances the cacTS2 synaptic transmission phenotype.

      There are no obvious changes in basic synapse morphology in cacTS2 P32EC1 double mutants.

      (Lutas et al., 2012)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      P32EC1/Df(2R)14F06W-07 is rescued by P32UAS.cLa/Scer\GAL4Appl.G1a

      (Lutas et al., 2012)
      Comments

      Scer\GAL4Appl.G1a-mediated expression of P32Scer\UAS.cLa rescues the paralytic and synaptic phenotypes of P32EC1/Df(2R)14F06W-07 animals.

      (Lutas et al., 2012)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (5)
      Reported As
      Symbol Synonym
      EC1
      (Lutas et al., 2012)
      P32EC1
      dp32EC1
      (Lutas et al., 2012)
      e(cac)2902
      (Lutas et al., 2012)
      Name Synonyms
      enhancer of cacTS2 1
      (Lutas et al., 2012)
      Secondary FlyBase IDs
        References (1)