FlyBase Allele Report: Dmel\klar[HMS01612]

General Information

Symbol

Dmel\klar^HMS01612

Species

D. melanogaster

Name

FlyBase ID

FBal0266519

Feature type

allele

Associated gene

Dmel\klar

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS01612}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

(Ni et al., 2011.7.19)

Progenitor genotype

Carried in construct

P{TRiP.HMS01612}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2011.7.19)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS01612

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

abdominal 1 lateral transverse muscle cell | embryonic stage, with Scer\GAL4^twi.PU

(Collins et al., 2017)

muscle cell of ventral longitudinal muscle 3 | larval stage, with Scer\GAL4^Mef2.PU

(Collins et al., 2017)

muscle cell of ventral longitudinal muscle 3 | larval stage, with Scer\GAL4^twi.PU

(Collins et al., 2017)

nucleus, with Scer\GAL4^Mef2.PU

(Collins et al., 2017)

nucleus, with Scer\GAL4^twi.PU

(Collins et al., 2017)

Detailed Description

Statement

Reference

VL3 muscles expressing klar^HMS01612 under the control of Scer\GAL4^Mef2.PU or Scer\GAL4^twi.PU exhibit nuclear positioning defects. Scer\GAL4^Mef2.PU-driven expression in larval VL3 muscles induces significantly shorter internuclear distances, longer nucleus-muscle edge distance, and shorter distance between nuclear lines, as compared to controls; embryonic LT muscles show no changes in the distances between the dorsal or ventral end of the muscle and the nearest nucleus, but nuclei cluster near the ventral end of the muscles. Scer\GAL4^twi.PU-driven expression in larval VL3 muscles induces shorter internuclear distances, but no changes in nucleus-muscle edge distance or distance between nuclear lines, as compared to controls; embryonic LT muscles show a significantly longer distance between the dorsal end of the muscle and the nearest nucleus and a significantly shorter distance between the ventral end of the muscle and the nearest nucleus.

(Collins et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions