FB2024_03 , released June 25, 2024
Allele: Dmel\mbcUAS.Tag:HA
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General Information
Symbol
Dmel\mbcUAS.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0266175
Feature type
allele
Associated gene
Dmel\mbc
Associated Insertion(s)
Carried in Construct
P{UAS-mbc.HA}
Also Known As
UAS-mbcHA
Key Links
Transgenic product class
cDNA
(Balagopalan et al., 2006)
wild_type
(Balagopalan et al., 2006)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Balagopalan et al., 2006)
wild_type
(Balagopalan et al., 2006)
Mutagen
in vitro construct
(Balagopalan et al., 2006)
Progenitor genotype
Carried in construct
P{UAS-mbc.HA}
Cytology
Description

UASt drives expression of the +521 to 7035 region of a 7035 bp mbc cDNA tagged with Tag:HA at the 3' end.

(Balagopalan et al., 2006)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
mbc
Tagged with
Tag:HA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      mbcUAS.Tag:HA/Scer\GAL4sns.PK rescues mbcD11.2

      (Haralalka et al., 2011)

      mbcUAS.Tag:HA/Scer\GAL4twi.PG rescues mbcD11.2

      (Balagopalan et al., 2006)

      mbcUAS.Tag:HA/Scer\GAL4how-24B rescues mbcD11.2/Df(3R)mbc9

      (Balagopalan et al., 2006)
      Fails to rescue

      mbcUAS.Tag:HA/Scer\GAL4kirre-rP298-G4 fails to rescue mbcD11.2

      (Haralalka et al., 2011)

      mbcUAS.Tag:HA/Scer\GAL4kirre-rP298-G4 fails to rescue mbcD11.2

      (Balagopalan et al., 2006)
      Comments

      Expression of mbcScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4sns.PK restores fusion in mbcD11.2 embryos and a wild-type somatic muscle pattern is formed.

      Myoblast fusion remains severely impaired in mbcD11.2 embryos expressing mbcScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4kirre-rP298. These embryos show a roughly twofold increase in muscle precursor formation (where a single fusion event between a founder cell and a fusion competent myoblast has occurred) compared to mbcD11.2 embryos.

      (Haralalka et al., 2011)

      Expression of mbcScer\UAS.T:Ivir\HA1 rescues the myoblast fusion defect in mbcD11.2 embryos when expressed using Scer\GAL4twi.PG or Scer\GAL4how-24B but not Scer\GAL4kirre-rP298.

      (Balagopalan et al., 2006)
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      mbcScer\UAS.T:Ivir\HA1
      mbcUAS.Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (3)