FlyBase Allele Report: Dmel\inc[1]

General Information

Symbol

Dmel\inc¹

Species

D. melanogaster

Name

FlyBase ID

FBal0266013

Feature type

allele

Associated gene

Dmel\inc

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Stavropoulos and Young, 2011)

Mutagen

ethyl methanesulfonate

(Stavropoulos and Young, 2011)

Progenitor genotype

Caused by aberration

Df(1)inc¹

Cytology

Description

257bp deletion between two divergently transcribed genes, CG14795 and inc. The transcription initiation site and 50bp of 5' untranslated region sequence of inc is removed by the deletion, while the 5' terminus and a small amount of upstream sequences of CG14795 remains intact. The level of both CG14795 and inc transcripts is reduced in the mutant.

(Stavropoulos and Young, 2011)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

X:1,512,837..1,513,093 [+]

Comment:

257 bp deletion removes a transcription start of inc and upstream region to CG14795.

(Stavropoulos and Young, 2011)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage

(Li et al., 2017)

abnormal neurophysiology | larval stage (with inc^f00285)

(Kikuma et al., 2019)

abnormal neurophysiology | third instar larval stage (with inc^f00285)

(Li et al., 2017)

abnormal sleep (with inc^f00285)

(Stavropoulos and Young, 2011)

abnormal sleep | adult stage

(Li et al., 2017)

abnormal sleep | recessive

(Stavropoulos and Young, 2011)

decreased sleep | adult stage

(Kikuma et al., 2019)

decreased sleep | adult stage (with inc^f00285)

(Kikuma et al., 2019)

decreased sleep | adult stage (with inc^kk3)

(Kikuma et al., 2019)

decreased sleep | adult stage (with inc^kk4)

(Kikuma et al., 2019)

short lived

(Stavropoulos and Young, 2011)

Phenotype Manifest In

calyx of adult mushroom body

(Vaughen et al., 2022)

lysosome | adult stage

(Vaughen et al., 2022)

NMJ bouton | increased number | third instar larval stage

(Li et al., 2017)

Detailed Description

Statement

Reference

inc¹ mutant adults sleep less (display reduced total amount of both daytime and nighttime sleep, along with short sleep bout length with increased bout number) than wild-type controls. Mutant third instar larvae show increased number of boutons on neuromuscular junction. Postsynaptic muscle recordings reveal altered synaptic transmission in inc¹/inc^f00285 larvae: the amplitude of spontaneous miniature postsynaptic potentials is comparable to controls but their frequency is reduced, the amplitude of evoked potentials is decreased as is the quantal content.

(Li et al., 2017)

These homozygotes are also short lived compared to controls.

(Stavropoulos and Young, 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

inc¹ has abnormal sleep | adult stage phenotype, suppressible | partially by Mmus\Kctd2^UAS.Tag:MYC/Scer\GAL4^inc.PS

(Li et al., 2017)

inc¹ has abnormal sleep | adult stage phenotype, suppressible | partially by Mmus\Kctd5^UAS.Tag:MYC/Scer\GAL4^inc.PS

(Li et al., 2017)

NOT suppressed by

Statement

Reference

inc¹ has abnormal sleep | adult stage phenotype, non-suppressible by Mmus\Kctd17^{UAS.3.Tag:MYC}/Scer\GAL4^inc.PS

(Li et al., 2017)

inc¹ has abnormal sleep | adult stage phenotype, non-suppressible by Mmus\Kctd17^{UAS.2.Tag:MYC}/Scer\GAL4^inc.PS

(Li et al., 2017)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The sleep deficit (reduced total amount of both daytime and nighttime sleep, along with short sleep bout length with increased bout number) characteristic for inc¹ mutant adults can be partially rescued by Scer\GAL4^inc.PS-driven expression of either Mmus\Kctd2^{Scer\UAS.T:Hsap\MYC} or Mmus\Kctd5^{Scer\UAS.T:Hsap\MYC} (but not Mmus\Kctd17^{Scer\UAS.3.T:Hsap\MYC} or Mmus\Kctd17^{Scer\UAS.2.T:Hsap\MYC}) in the mutant background.

(Li et al., 2017)

Complementation and Rescue Data

Rescued by

inc¹ is rescued by inc^{UAS.10xFLAGsmFP}/Scer\GAL4^inc.PS

(Kikuma et al., 2019)

inc¹ is rescued by inc^UAS.Tag:MYC/Scer\GAL4^inc.PS

(Li et al., 2017)

inc¹ is rescued by inc^UAS.Tag:FLAG/Scer\GAL4^inc.PS

(Li et al., 2017)

Comments

The sleep deficit (reduced total amount of both daytime and nighttime sleep, along with short sleep bout length with increased bout number) characteristic for inc¹ mutant adults is rescued by expression of either inc^{Scer\UAS.T:Hsap\MYC} or inc^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^inc.PS.

(Li et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

inc¹

(Durkin et al., 2023, De Nobrega et al., 2022, Vaughen et al., 2022, Li et al., 2021, Li et al., 2021, Kikuma et al., 2019, Li et al., 2017, Stavropoulos and Young, 2011)

insomniac¹

(Axelrod et al., 2023)

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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