FB2024_03 , released June 25, 2024
Allele: Dmel\gekUAS.cGa
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General Information
Symbol
Dmel\gekUAS.cGa
Species
D. melanogaster
Name
FlyBase ID
FBal0265911
Feature type
allele
Associated gene
Dmel\gek
Associated Insertion(s)
Carried in Construct
P{UAS-gek.G}
Key Links
Transgenic product class
cDNA
(Gontang et al., 2011)
wild_type
(Gontang et al., 2011)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Gontang et al., 2011)
wild_type
(Gontang et al., 2011)
Mutagen
in vitro construct
(Gontang et al., 2011)
Progenitor genotype
Carried in construct
P{UAS-gek.G}
Cytology
Description

UAS regulatory sequences drive expression of a gek cDNA.

(Gontang et al., 2011)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
gek
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of gekScer\UAS.cGa under the control of Scer\GAL4Glass38-1 has no effect on axon targeting in wild type third instar larvae. However approximately 28% of adult R7 cells expressing gekScer\UAS.cGa target either to the R8 layer or to an even more superficial layer in the outer medulla, or occasionally to the lamina. R8 axon targeting is unaffected.

      (Gontang et al., 2011)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Suppressor of
      Statement
      Reference

      Scer\GAL4elav-C155/gekUAS.cGa is a non-suppressor of abnormal neuroanatomy | somatic clone phenotype of Cdc424

      (Gontang et al., 2011)
      Phenotype Manifest In
      NOT Suppressor of
      Statement
      Reference

      Scer\GAL4elav-C155/gekUAS.cGa is a non-suppressor of eye photoreceptor cell | somatic clone phenotype of Cdc424

      (Gontang et al., 2011)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of gekScer\UAS.cGa under the control of Scer\GAL4elav-C155 fails to suppress the axon targeting phenotypes seen in Cdc424 mutant clones.

      (Gontang et al., 2011)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4elav-C155/gekUAS.cGa rescues gekomb1080

      (Gontang et al., 2011)

      gekUAS.cGa rescues gekomb1080/Df(2R)e03819-f02302

      (Gontang et al., 2011)
      Comments

      Expression of gekScer\UAS.cGa under the control of Scer\GAL4Glass38-1 rescues the axon targeting phenotypes seen in gekomb1080/Df(2R)e03819-f02302 third instar larvae.

      Expression of gekScer\UAS.cGa in R cells under the control of Scer\GAL4elav-C155 rescues the axon targeting phenotypes seen in gekomb1080 homozygous pupal stage clones. 91% of axons extend correctly, only 3% mistarget and 6% do not extend. Premature stopping of R1-R6 axons above the lamina plexus is not observed.

      (Gontang et al., 2011)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      gekScer\UAS.cGa
      gekUAS.cGa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Gontang
      (Gontang et al., 2011)
      Secondary FlyBase IDs
        References (1)