The expression of Hsap\FUSScer\UAS.10x.T:Zzzz\FLAG under the control of Scer\GAL4Toll-6-D42 does not lead to any obvious defects in viability, third instar larval locomotion or adult locomotion, as compared to controls.
The expression of Hsap\FUSScer\UAS.10x.T:Zzzz\FLAG under the control of Scer\GAL4CCAP.PI leads to a decrease in axonal transport of NPY-positive vesicles (i.e. a significant decrease in anterograde transport and a significant increase in the stationary fraction), but not of mitochondria, in third instar larval motor neurons, as compared to controls.
Expression of Hsap\FUSScer\UAS.10x.T:Zzzz\FLAG in motor neurons under the control of Scer\GAL4D42 results in impaired larval locomotion, which is rescued by pioglitazone-treatment. However, pioglitazone-treatment is not effective in improving lifespan in adult transgenic animals.
Expression of Hsap\FUSScer\UAS.10x.T:Zzzz\FLAG in motor neurons under the control of Scer\GAL4VGlut-OK371 results in a slight increase in motility in third instar larvae and young adults compared with controls.
Scer\GAL4VGlut-OK371>Hsap\FUSScer\UAS.10x.T:Zzzz\FLAG-expressing animals display a reduction in the number of presynaptic active zones compared with wild-type.
Compared with wild-type, third instar larvae expressing Hsap\FUSScer\UAS.10x.T:Zzzz\FLAG under the control of Scer\GAL4VGlut-OK371 display an approximately 25% reduction in evoked excitatory junction potential (EJP) amplitude. Hsap\FUSScer\UAS.10x.T:Zzzz\FLAG-expression does not cause a significant alteration in EJP kinetics.
Analysis of synaptic fatigue reveals that animals expressing Scer\GAL4VGlut-OK371>Hsap\FUSScer\UAS.10x.T:Zzzz\FLAG fatigue faster than control animals.
Overexpression of Hsap\FUSScer\UAS.10x.T:Zzzz\FLAG in motor neurons via Scer\GAL4RapGAP1-OK6 induces neuromuscular junction (NMJ) expansion.
Overexpression of Hsap\FUSScer\UAS.10x.T:Zzzz\FLAG in neurons has no significant effect on locomotion.
Overexpression of Hsap\FUSScer\UAS.10x.T:Zzzz\FLAG has no effect on longevity.
Hsap\FUSUAS.10x.Tag:FLAG, Scer\GAL4GMR.PS is a suppressor | partially of decreased size | adult stage phenotype of Scer\GAL4GMR.PS, Zzzz\GGGGCC89.UAS
Hsap\FUSUAS.10x.Tag:FLAG, Scer\GAL4GMR.PS is a suppressor | partially of abnormal eye color phenotype of Scer\GAL4GMR.PS, Zzzz\GGGGCC42.UAS
Hsap\FUSUAS.10x.Tag:FLAG, Scer\GAL4GMR.PS is a suppressor | partially of abnormal eye color phenotype of Scer\GAL4GMR.PS, Zzzz\GGGGCC89.UAS
Hsap\FUSUAS.10x.Tag:FLAG/Scer\GAL4Toll-6-D42 is a suppressor of partially lethal - majority die phenotype of caz383
Hsap\FUSUAS.10x.Tag:FLAG/Scer\GAL4Toll-6-D42 is a suppressor | partially of abnormal locomotor behavior | adult stage phenotype of caz383
Hsap\FUSUAS.10x.Tag:FLAG/Scer\GAL4Toll-6-D42 is a suppressor | partially of abnormal locomotor behavior | third instar larval stage phenotype of caz383
Scer\GAL4elav-C155/Hsap\FUSUAS.10x.Tag:FLAG is a suppressor of partially lethal - majority die phenotype of CG32576+t5.0, caz383
Scer\GAL4elav-C155/Hsap\FUSUAS.10x.Tag:FLAG is a suppressor | partially of abnormal locomotor behavior phenotype of CG32576+t5.0, caz383
Scer\GAL4elav-C155/Hsap\FUSUAS.10x.Tag:FLAG is a suppressor of short lived phenotype of CG32576+t5.0, caz383
Hsap\FUSUAS.10x.Tag:FLAG, Scer\GAL4GMR.PS is a suppressor | partially of eye phenotype of Scer\GAL4GMR.PS, Zzzz\GGGGCC89.UAS
Hsap\FUSUAS.10x.Tag:FLAG, Scer\GAL4GMR.PS is a suppressor | partially of eye phenotype of Scer\GAL4GMR.PS, Zzzz\GGGGCC42.UAS
Scer\GAL4CCAP.PU/Hsap\FUSUAS.10x.Tag:FLAG is a suppressor of embryonic/larval motor neuron | third instar larval stage phenotype of caz383
The expression of Hsap\FUSScer\UAS.10x.T:Zzzz\FLAG under the control of Scer\GAL4Toll-6-D42 fully/nearly fully suppresses the semi-lethality, the decreased third instar larval crawling capacity and the decreased adult locomotor capacity observed in caz383 homozygotes.
The expression of Hsap\FUSScer\UAS.10x.T:Zzzz\FLAG under the control of Scer\GAL4CCAP.PI suppresses the decreased axonal transport of mitochondria and (NPY-positive) vesicles observed in caz383 homozygous third instar larval motor neurons.
Expression of Hsap\FUSScer\UAS.10x.T:Zzzz\FLAG in the nervous system under the control of Scer\GAL4elav-C155 almost completely rescues the adult viability of males hemizygous for the 'caz1' mutant chromosome (Df(1)383 onto which a transgene containing CG32576+t5.0 has been recombined to provide CG32576[+] function) - the level of rescue seen with Hsap\FUSScer\UAS.10x.T:Zzzz\FLAG is not significantly different from that seen when a transgene containing wild-type caz is expressed using the same driver.
Expression of Hsap\FUSScer\UAS.10x.T:Zzzz\FLAG under the control of Scer\GAL4elav-C155 strongly rescues the locomotor defects seen in caz1/Y adult males, to a level that is not significantly different from that seen when a transgene containing wild-type caz is expressed using the same driver.
Expression of Hsap\FUSScer\UAS.10x.T:Zzzz\FLAG under the control of Scer\GAL4elav-C155 completely rescues the reduced longevity of adult caz1/Y escapers.