FlyBase Allele Report: Dmel\Rae1[EX28]

General Information

Symbol

Dmel\Rae1^EX28

Species

D. melanogaster

Name

FlyBase ID

FBal0265658

Feature type

allele

Associated gene

Dmel\Rae1

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Tian et al., 2011)

Mutagen

P-element activity

(Tian et al., 2011)

Progenitor genotype

Cytology

Description

Imprecise excision of P{GawB}NP3499 (located ~400bp upstream of the first Rae1 exon) generates an approximate 900bp deletion ranging from just before the first exon of Rae1 (including almost the entire Rae1 promoter) to the end of adjacent pirk.

(Tian et al., 2011)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal body size | second instar larval stage

(Tian et al., 2011)

lethal - all die before end of second instar larval stage

(Tian et al., 2011)

some die during second instar larval stage

(Tian et al., 2011)

Phenotype Manifest In

embryonic/larval neuromuscular junction

(Tian et al., 2011)

embryonic/larval neuromuscular junction (with Df(2R)XE-2900)

(Tian et al., 2011)

embryonic/larval somatic muscle cell

(Tian et al., 2011)

NMJ bouton

(Tian et al., 2011)

NMJ bouton (with Df(2R)XE-2900)

(Tian et al., 2011)

synapse

(Tian et al., 2011)

synapse (with Df(2R)XE-2900)

(Tian et al., 2011)

Detailed Description

Statement

Reference

Rae1^EX28 mutants survive until the second instar larval stage. The mutant larvae start wandering with a body size close to second instar. They continue to wander for several days without a further increase in their body size and eventually die before pupation.

Rae1^EX28 and Rae1^EX28/Df(2R)XE-2900 mutant neuromuscular junctions exhibit synaptic terminal overgrowth and decreased bouton size in synapses on muscle 6/7. The average muscle size in these mutants appears similar to controls.

(Tian et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

Rae1^EX28 has synapse phenotype, suppressible by wnd³

(Tian et al., 2011)

Rae1^EX28 has NMJ bouton phenotype, suppressible by wnd³

(Tian et al., 2011)

Rae1^EX28 has synapse phenotype, suppressible by Atg1³/Atg1[+]

(Tian et al., 2011)

Rae1^EX28 has NMJ bouton phenotype, suppressible by Atg1³/Atg1[+]

(Tian et al., 2011)

Rae1^EX28 has synapse phenotype, suppressible by Atg2[+]/Atg2^EP3697

(Tian et al., 2011)

Rae1^EX28 has NMJ bouton phenotype, suppressible by Atg2[+]/Atg2^EP3697

(Tian et al., 2011)

Rae1^EX28 has synapse phenotype, suppressible by Atg18a^KG03090/Atg18[+]

(Tian et al., 2011)

Rae1^EX28 has NMJ bouton phenotype, suppressible by Atg18a^KG03090/Atg18[+]

(Tian et al., 2011)

NOT suppressed by

Statement

Reference

Rae1^EX28 has synapse phenotype, non-suppressible by Scer\GAL4^elav-C155/hiw^UAS.W.EGFP

(Tian et al., 2011)

Rae1^EX28 has NMJ bouton phenotype, non-suppressible by Scer\GAL4^elav-C155/hiw^UAS.W.EGFP

(Tian et al., 2011)

Enhancer of

Statement

Reference

Rae1[+]/Rae1^EX28 is an enhancer of embryonic/larval neuromuscular junction phenotype of hiw^ND51

(Tian et al., 2011)

Rae1[+]/Rae1^EX28 is an enhancer of synapse phenotype of hiw^ND51

(Tian et al., 2011)

Rae1[+]/Rae1^EX28 is an enhancer of NMJ bouton phenotype of hiw^ND51

(Tian et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

A wnd³ background suppresses the bouton number increase and the reduction in bouton size seen in Rae1^EX28 mutants.

Presynaptic expression of hiw^{Scer\UAS.W.T:Avic\GFP-EGFP} under the control of Scer\GAL4^elav-C155 fails to rescue the bouton number increase found in Rae1^EX28 mutant synapses.

An Atg1³ heterozygous background dominantly suppresses the increase in bouton number seen in Rae1^EX28 homozygous mutant neuromuscular junction.

An Atg2^EP3697 heterozygous background dominantly suppresses the increase in bouton number seen in Rae1^EX28 homozygous mutant neuromuscular junction.

An Atg18^KG03090 heterozygous background dominantly suppresses the increase in bouton number seen in Rae1^EX28 homozygous mutant neuromuscular junction.

(Tian et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Rae1^EX28 is rescued by Scer\GAL4^elav-C155/Rae1^UAS.Tag:TAP

(Tian et al., 2011)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Rae1^EX28

(Martinez et al., 2021, Tian et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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