FB2024_03 , released June 25, 2024
Allele: Dmel\RanQ69L.UASp.Tag:HA
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General Information
Symbol
Dmel\RanQ69L.UASp.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0265627
Feature type
allele
Associated gene
Dmel\Ran
Associated Insertion(s)
Carried in Construct
P{UASp-Ran.Q69L}
Key Links
Transgenic product class
missense_variant
(Cesario and McKim, 2011)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Cesario and McKim, 2011)
Mutagen
in vitro construct
(Cesario and McKim, 2011)
Progenitor genotype
Carried in construct
P{UASp-Ran.Q69L}
Cytology
Description

UASp regulatory sequences drive expression of a GTP-locked form of Ran (amino acid replacement Q69L) which is tagged at the N-terminal end with three copies of Tag:HA.

(Cesario and McKim, 2011)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
Ran
Tagged with
Tag:HA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The expression of RanQ69L.UASp.Tag:HA under the control of Scer\GAL4VP16.nos.UTR induces clustering of annulate lamellae at the oocyte anterior.

      (Hampoelz et al., 2019)

      Expression of ranQ69L.Scer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 results in complete female sterility.

      Expression of ranQ69L.Scer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 results in defects in spindle morphology in the oocyte (the microtubules fail to taper properly at the spindle poles) and the karyosome is disorganised.

      The majority of zygotes derived from females expressing ranQ69L.Scer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 contain a cluster of DNA and microtubules in the centre of the cell without any evidence of mitotic divisions.

      (Cesario and McKim, 2011)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      RanQ69L.Scer\UAS.P\T.T:Ivir\HA1
      RanQ69L.UASp.Tag:HA
      ranQ69L.Scer\UAS.P\T.T:Ivir\HA1
      ranQ69L
      (Cesario and McKim, 2011)
      Name Synonyms
      Secondary FlyBase IDs
        References (3)