FB2024_03 , released June 25, 2024
Allele: Dmel\pblΔBRCT.UAS.Tag:MYC
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General Information
Symbol
Dmel\pblΔBRCT.UAS.Tag:MYC
Species
D. melanogaster
Name
FlyBase ID
FBal0263870
Feature type
allele
Associated gene
Dmel\pbl
Associated Insertion(s)
Carried in Construct
P{UAS-pbl.ΔBRCT.Myc}
Key Links
Transgenic product class
inframe_deletion
(van Impel et al., 2009)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(van Impel et al., 2009)
Mutagen
in vitro construct
(van Impel et al., 2009)
Progenitor genotype
Carried in construct
P{UAS-pbl.ΔBRCT.Myc}
Cytology
Description

UASt regulatory sequences drive expression of a mutant form of pbl that lacks the two N-terminal BRCT domains and is tagged with Tag:MYC

(van Impel et al., 2009)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
pbl
Tagged with
Tag:MYC
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Scer\GAL4e22c-mediated expression of pblΔBRCT.Scer\UAS.T:Hsap\MYC results in 95.7% embryonic lethality with 40.6% of embryos showing some loss of naked cuticle on the ventral side. Other defects include missing head structures, failure to retract the germ band and incomplete dorsal closure.

      (Greer et al., 2013)

      No defects in early mesodermal development are observed when pblΔBRCT.Scer\UAS.T:Hsap\MYC is expressed in stage 8 embryos under the control of the Scer\GAL4twi.2PE and Scer\GAL4Mef2.PR drivers.

      (van Impel et al., 2009)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      NOT suppressed by
      Phenotype Manifest In
      Suppressed by
      Statement
      Reference

      Scer\GAL4twi.2PE, pblΔBRCT.UAS.Tag:MYC, pbl3 has mesoderm phenotype, suppressible by Rac1UAS.cLa

      (van Impel et al., 2009)
      NOT suppressed by
      Statement
      Reference

      Scer\GAL4twi.2PE, pblΔBRCT.UAS.Tag:MYC, pbl3 has mesoderm phenotype, non-suppressible by Rho1UAS.cMa

      (van Impel et al., 2009)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Scer\GAL4arm.PS-mediated co-expression of armS10.Scer\UAS.T:Hsap\MYC and pblΔBRCT.Scer\UAS.T:Hsap\MYC produces severe developmental defects.

      (Greer et al., 2013)

      Co-expression of Rac1Scer\UAS.cLa enhances the partial rescue of pbl3 mesodermal migration defects seen when pblΔBRCT.Scer\UAS.T:Hsap\MYC is expressed under the control of Scer\GAL4twi.2PE.

      Co-expression of Rho1Scer\UAS.cMa does not enhance the partial rescue of pbl3 mesodermal migration defects seen when pblΔBRCT.Scer\UAS.T:Hsap\MYC is expressed under the control of Scer\GAL4twi.2PE.

      (van Impel et al., 2009)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      pblΔBRCT.UAS.Tag:MYC/Scer\GAL4twi.2PE partially rescues pbl3

      (van Impel et al., 2009)
      Fails to rescue

      pblΔBRCT.UAS.Tag:MYC/Scer\GAL4twi.2PE fails to rescue pbl3

      (van Impel et al., 2009)
      Comments

      Expression of pblΔBRCT.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4twi.2PE partially rescues the mesodermal migration defects seen in pbl3 homozygous mutants but is unable to rescue the cytokinesis defects.

      (van Impel et al., 2009)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      pblΔBRCT.Scer\UAS.T:Hsap\MYC
      pblΔBRCT.UAS.Tag:MYC
      Name Synonyms
      Secondary FlyBase IDs
        References (2)