FB2024_03 , released June 25, 2024
Allele: Dmel\VavUAS.Tag:HA
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General Information
Symbol
Dmel\VavUAS.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0263651
Feature type
allele
Associated gene
Dmel\Vav
Associated Insertion(s)
Carried in Construct
P{UAS-Vav.HA}
Key Links
Transgenic product class
missense_variant
(Couceiro et al., 2005)
silent_mutation
(Couceiro et al., 2005)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Couceiro et al., 2005)
silent_mutation
(Couceiro et al., 2005)
Mutagen
in vitro construct
(Couceiro et al., 2005)
Progenitor genotype
Carried in construct
P{UAS-Vav.HA}
Cytology
Description

Amino acid replacement: M53T.

(Couceiro et al., 2005)

A 3.0kb full length Vav coding sequence is expressed under the control of UASt regulatory sequences and tagged at the C-terminal with Tag:HA. Two alterations to the sequence are present, causing M53T and a silent codon change in codon 313.

(Couceiro et al., 2005)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Vav
Tagged with
Tag:HA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Embryos expressing vavScer\UAS.T:Ivir\HA1 in the epidermis using the Scer\GAL469B driver are indistinguishable from wild type.

      Embryos expressing vavScer\UAS.T:Ivir\HA1 in the mesoderm using the Scer\GAL4how-24B driver do not display any obvious muscle defects.

      Embryos expressing vavScer\UAS.T:Ivir\HA1 under the control of the nervous system driver Scer\GAL4elav.PLu are indistinguishable from wild type.

      Caudal visceral cell (CVM) migration is comparable to wild type when vavScer\UAS.T:Ivir\HA1 is expressed in embryos under the control of the Scer\GAL4447.2 driver.

      Expression of vavScer\UAS.T:Ivir\HA1 under the control of the tracheal system driver Scer\GAL4btl.PS has no effect on tracheal development.

      (Couceiro et al., 2005)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4arm.PS/VavUAS.Tag:HA rescues Vav2

      (Malartre et al., 2010)

      VavUAS.Tag:HA/Scer\GAL4αTub84B.PL rescues Vav2

      (Malartre et al., 2010)

      Scer\GAL4arm.PS/VavUAS.Tag:HA rescues Vav3

      (Malartre et al., 2010)

      VavUAS.Tag:HA/Scer\GAL4αTub84B.PL rescues Vav3

      (Malartre et al., 2010)
      Partially rescues

      Scer\GAL4arm.PS/VavUAS.Tag:HA partially rescues Vav2

      (Malartre et al., 2010)

      Scer\GAL4arm.PS/VavUAS.Tag:HA partially rescues Vav3

      (Malartre et al., 2010)
      Fails to rescue

      VavUAS.Tag:HA/Scer\GAL4GMR.PU fails to rescue Vav1

      (Malartre et al., 2010)
      Comments

      Expression of vavScer\UAS.T:Ivir\HA1 under the control of either the Scer\GAL4arm.PS or the Scer\GAL4αTub84B.PL driver rescues the semi-lethality seen in vav2 male homozygotes.

      Expression of vavScer\UAS.T:Ivir\HA1 under the control of either the Scer\GAL4arm.PS or the Scer\GAL4αTub84B.PL driver rescues the semi-lethality seen in vav3 male homozygotes.

      Expression of vavScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4arm.PS partially rescues the midline crossing phenotype seen in vav2 homozygotes.

      Expression of vavScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4arm.PS partially rescues the midline crossing phenotype seen in vav3 homozygotes.

      Expression of vavScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PU fails to rescue the photoreceptor targeting phenotype seen in vav1 homozygotes.

      (Malartre et al., 2010)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      VavScer\UAS.T:Ivir\HA1
      VavUAS.Tag:HA
      vavScer\UAS.T:Ivir\HA1
      Name Synonyms
      Secondary FlyBase IDs
        References (2)