FlyBase Allele Report: Dmel\Rab2[HMS01271]

General Information

Symbol

Dmel\Rab2^HMS01271

Species

D. melanogaster

Name

FlyBase ID

FBal0263282

Feature type

allele

Associated gene

Dmel\Rab2

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS01271}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

(Ni et al., 2011.7.19)

Progenitor genotype

Carried in construct

P{TRiP.HMS01271}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2011.7.19)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS01271

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^Act5C.PU

(Mallik et al., 2017)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^Mef2.PU

(Mallik et al., 2017)

Phenotype Manifest In

Detailed Description

Statement

Reference

The expression of Rab2^HMS01271 under the control of Scer\GAL4^{SPARC-MI00329-GAL4} (and Dicer-2, for a more efficient RNAi) in the larval fat body induces defective secretion, including extracellular matrix material, as shown by the retention of both secr.GFP and Vkg.GFP. Expression under the control of Scer\GAL4^Cg.PA alone leads to an increase in the size of the cis-Golgi (Grasp65.RFP) without a similar effect on endoplasmic reticulum exit sites (Sec16.GFP).

(Ke et al., 2018)

Expression of Rab2^HMS01271 under the control of either Scer\GAL4^Act5C.PU or Scer\GAL4^Mef2.PU results in smaller synapse size and reduced number of boutons on neuromuscular junctions in third instar larvae compared to controls.

(Mallik et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Rab2^HMS01271, Scer\GAL4^Act5C.PU has abnormal neuroanatomy | third instar larval stage phenotype, non-enhanceable by ICA69^GD7020, Scer\GAL4^Act5C.PU

(Mallik et al., 2017)

Suppressed by

Statement

Reference

Rab2^HMS01271, Scer\GAL4^Act5C.PU has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by ICA69^UAS.FL, Scer\GAL4^Act5C.PU

(Mallik et al., 2017)

NOT Enhancer of

Statement

Reference

Rab2^HMS01271, Scer\GAL4^Act5C.PU is a non-enhancer of abnormal neuroanatomy | third instar larval stage phenotype of ICA69^GD7020, Scer\GAL4^Act5C.PU

(Mallik et al., 2017)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Rab2^HMS01271, Scer\GAL4^Act5C.PU has NMJ bouton | third instar larval stage phenotype, non-enhanceable by ICA69^GD7020, Scer\GAL4^Act5C.PU

(Mallik et al., 2017)

Rab2^HMS01271, Scer\GAL4^Act5C.PU has embryonic/larval neuromuscular junction | third instar larval stage phenotype, non-enhanceable by ICA69^GD7020, Scer\GAL4^Act5C.PU

(Mallik et al., 2017)

Suppressed by

Statement

Reference

Rab2^HMS01271, Scer\GAL4^Act5C.PU has NMJ bouton | third instar larval stage phenotype, suppressible by ICA69^UAS.FL, Scer\GAL4^Act5C.PU

(Mallik et al., 2017)

Rab2^HMS01271, Scer\GAL4^Act5C.PU has embryonic/larval neuromuscular junction | third instar larval stage phenotype, suppressible by ICA69^UAS.FL, Scer\GAL4^Act5C.PU

(Mallik et al., 2017)

NOT Enhancer of

Statement

Reference

Rab2^HMS01271, Scer\GAL4^Act5C.PU is a non-enhancer of NMJ bouton | third instar larval stage phenotype of ICA69^GD7020, Scer\GAL4^Act5C.PU

(Mallik et al., 2017)

Rab2^HMS01271, Scer\GAL4^Act5C.PU is a non-enhancer of embryonic/larval neuromuscular junction | third instar larval stage phenotype of ICA69^GD7020, Scer\GAL4^Act5C.PU

(Mallik et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The neuromuscular junction (NMJ) structural defects (smaller synapse, reduced number of boutons) characteristic for third instar larvae expressing either Rab2^HMS01271 or ICA69^GD7020 under the control of Scer\GAL4^Act5C.PU are not further worsened when the two RNAi alleles are co-expressed. However, the Rab2^HMS01271 RNAi-induced NMJ defects can be rescued by co-expression of ICA69^Scer\UAS.FL.

(Mallik et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

34922

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01271}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Rab2^HMS01271

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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