FB2024_03 , released June 25, 2024
Allele: Dmel\cherCPTI001399
Open Close
General Information
Symbol
Dmel\cherCPTI001399
Species
D. melanogaster
Name
FlyBase ID
FBal0262157
Feature type
allele
Associated gene
Dmel\cher
Associated Insertion(s)
PBac{681.P.FSVS-1}cherCPTI001399
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
piggyBac activity
(FlyProt project., 2011.8.8)
Progenitor genotype
Associated Insertion(s)
PBac{681.P.FSVS-1}cherCPTI001399
Cytology
Description

Protein trap insertion line, in-frame.

(FlyProt project., 2011.8.8)
Allele components
Component
Use(s)
Inserted element
PBac{681.P.FSVS-1}
Tagged with
Tag:FLAG
Tag:StrepII
Venus
Mutations Mapped to the Genome
Curation Data
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      cher protein knock-down by using slmbαTub67C.vhhGFP4 in a cherCPTI001399 homozygous background results in failure to assemble a hexagonal network during cellularization, and leads to premature rounding and constriction; in addition, there is a delay in ventral furrow invagination during gastrulation. On STED super-resolution microscopy, actin filaments fail to acquire the hexagonal conformation observed in controls and instead localize in a diffuse meshwork that occupies the space separating individual nuclei; actin fibers appear less thick than in controls.

      (Krueger et al., 2019)

      cherCPTI001399 homozygotes are female fertile.

      (González-Morales et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The increased/premature actomyosin constriction observed in Df(3R)Exel6218 homozygous embryos or in cher protein depleted embryos (i.e. cherCPTI001399, slmbαTub67C.vhhGFP4 embryos) is enhanced in the double mutant background.

      (Krueger et al., 2019)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      cher1399
      (González-Morales et al., 2017)
      cherCPTI001399
      (Huelsmann et al., 2013)
      cherCPTI1399
      (González-Morales et al., 2017)
      Name Synonyms
      Secondary FlyBase IDs
        References (4)