UAS regulatory sequences drive expression of wild-type Hsap\TARDBP coding sequences where the nuclear export signal (NES) is mutated. The protein is tagged with Tag:MYC.
Eye degeneration used to model disease.
Expression of Hsap\TARDBPmutNES.Scer\UAS in the developing eye under the control of Scer\GAL4GMR.PF results in a normal eye phenotype.
Hsap\TARDBPNESmut.UAS.Tag:MYC, Scer\GAL4GMR.PF has increased cell death phenotype, non-enhanceable by TER94UAS.cRa, Scer\GAL4GMR.PF
Hsap\TARDBPNESmut.UAS.Tag:MYC, Scer\GAL4GMR.PF has increased cell death phenotype, non-suppressible by TER94UAS.cRa, Scer\GAL4GMR.PF
Hsap\TARDBPNESmut.UAS.Tag:MYC, Scer\GAL4GMR.PF has eye phenotype, non-enhanceable by TER94UAS.cRa, Scer\GAL4GMR.PF
Hsap\TARDBPNESmut.UAS.Tag:MYC, Scer\GAL4GMR.PF has ommatidium phenotype, non-enhanceable by TER94UAS.cRa, Scer\GAL4GMR.PF
Hsap\TARDBPNESmut.UAS.Tag:MYC, Scer\GAL4GMR.PF has eye phenotype, non-suppressible by TER94UAS.cRa, Scer\GAL4GMR.PF
Hsap\TARDBPNESmut.UAS.Tag:MYC, Scer\GAL4GMR.PF has ommatidium phenotype, non-suppressible by TER94UAS.cRa, Scer\GAL4GMR.PF
Co-expression of TER94Scer\UAS.cRa and Hsap\TARDBPmutNES.Scer\UAS in the developing eye under the control of Scer\GAL4GMR.PF doesn't generate an eye phenotype, indicating there is no interaction between these transgenes.