FlyBase Allele Report: Scer\GAL4[alrm.PD]

General Information

Symbol

Scer\GAL4^alrm.PD

Species

S. cerevisiae

Name

astrocytic leucine-rich repeat molecule promoter construct of Doherty

FlyBase ID

FBal0260884

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{alrm-GAL4.D}

Also Known As

alrm-Gal4, alrmGal4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Doherty et al., 2009)

Progenitor genotype

Carried in construct

P{alrm-GAL4.D}

Cytology

Description

A 4973bp region of the alrm promoter is fused upstream of sequence encoding a GAL4 driver.

(Doherty et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

alrm

alrm_upstream-Gal4

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

pupal stage

astrocyte-like glial cell

(Tasdemir-Yilmaz and Freeman, 2014)

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

larval neuropil glial cell | subset

(Losada-Perez et al., 2016)

pharate adult stage P8 -- P15

astrocyte-like glial cell

(Muthukumar et al., 2014)

Additional Information

Statement

Reference

Drives expression in reticular neuropil associated glial cells.

(FlyBase Curators, 2017)

The larval neuropil glial cells are Gs2-positive and comprise ScerGAL4^alrm.PD-positive, pros-positive and ScerGAL4^NP6520-positive, pros-negative subsets.

(Losada-Perez et al., 2016)

ScerGAL4^alrm.PD drives expression in a subset of distal medulla astrocyte-like glial cells with processes to medulla layer M7.

(Edwards et al., 2012)

Marker for

astrocyte-like glial cell

(Muthukumar et al., 2014, Stork et al., 2014, Tasdemir-Yilmaz and Freeman, 2014, Doherty et al., 2009)

Reflects expression of

alrm

(Muthukumar et al., 2014, Edwards et al., 2012, Doherty et al., 2009)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal circadian rhythm | adult stage, with Cpes^RNAi.UAS.IR1

(Chen et al., 2022)

abnormal circadian rhythm | adult stage, with Cpes^RNAi.UAS.IR2

(Chen et al., 2022)

abnormal circadian rhythm | adult stage, with Manf^UAS.cPa

(Walkowicz et al., 2021)

abnormal circadian rhythm | adult stage, with Nos^GD12013

(Kozlov et al., 2020)

abnormal heat stress response | adult stage, with Hsap\SLC6A1^{A288V.UAS.YFP}

(Kasture et al., 2022)

abnormal locomotor behavior | adult stage, with Manf^GD4793

(Walkowicz et al., 2021)

abnormal locomotor behavior | adult stage, with Manf^UAS.cPa

(Walkowicz et al., 2021)

abnormal locomotor behavior | third instar larval stage, with Gat^GD5029

(Stork et al., 2014)

abnormal locomotor behavior | third instar larval stage, with Gat^JF03358

(Stork et al., 2014)

abnormal locomotor behavior | third instar larval stage, with Gat^KK107895

(Stork et al., 2014)

abnormal locomotor rhythm | heat sensitive, with Scer\GAL80^ts.αTub84B, shi^1.UAS

(Ng et al., 2011)

abnormal neuroanatomy, with Ced-12^KK102788

(Tasdemir-Yilmaz and Freeman, 2014)

abnormal neuroanatomy, with Crk^GD8583

(Tasdemir-Yilmaz and Freeman, 2014)

abnormal neuroanatomy, with drpr^RNAi.UAS

(Tasdemir-Yilmaz and Freeman, 2014)

abnormal neuroanatomy, with EcR^{B1-ΔC655.F645A.UAS}

(Tasdemir-Yilmaz and Freeman, 2014)

abnormal neuroanatomy, with EcR^{B1-ΔC655.W650A.UAS}

(Tasdemir-Yilmaz and Freeman, 2014)

abnormal neuroanatomy, with mbc^GD6965

(Tasdemir-Yilmaz and Freeman, 2014)

abnormal neuroanatomy, with shi^1.UAS

(Tasdemir-Yilmaz and Freeman, 2014)

abnormal neuroanatomy | adult stage, with Atg5^HMS01244

(Damulewicz et al., 2022)

abnormal neuroanatomy | adult stage, with Atg7^JF02787

(Damulewicz et al., 2022)

abnormal neuroanatomy | adult stage, with Hsap\GFAP^R79H.UAS

(Wang et al., 2015)

abnormal neuroanatomy | heat sensitive, with shi^1.UAS

(Kazama et al., 2011)

abnormal neuroanatomy | larval stage, with CG12926^KK106958

(Yin et al., 2021)

abnormal neuroanatomy | larval stage, with CG30392^KK101488

(Yin et al., 2021)

abnormal neuroanatomy | larval stage, with GLaz^HMC06329

(Yin et al., 2021)

abnormal neuroanatomy | larval stage, with GLaz^KK106377

(Yin et al., 2021)

abnormal neurophysiology | adult stage | heat sensitive, with TrpA1^UAS.(B).cKa

(Liu et al., 2014)

abnormal phototaxis | adult stage, with sws^GD3277

(Dutta et al., 2016)

abnormal sleep | adult stage, with Atg5^HMS01244

(Damulewicz et al., 2022)

abnormal sleep | adult stage, with Atg7^JF02787

(Damulewicz et al., 2022)

bang sensitive, with kcc^HMS01058

(Rusan et al., 2014)

decreased efficacy of long-term memory | adult stage, with Alat^GD9174

(Rabah et al., 2023)

decreased efficacy of long-term memory | adult stage, with Alat^HMC05124

(Rabah et al., 2023)

decreased rate of movement | adult stage, with speck^HMS01617

(Davla et al., 2020)

decreased rate of movement | adult stage, with speck^JF02142

(Davla et al., 2020)

decreased sleep | adult stage, with Manf^UAS.cPa

(Walkowicz et al., 2021)

increased cell number, with Pvf2^d02444

(Winkler et al., 2021)

increased sleep | adult stage, with Atg5^HMS01244

(Damulewicz et al., 2022)

increased sleep | adult stage, with Atg7^JF02787

(Damulewicz et al., 2022)

increased sleep | adult stage | conditional, with speck^HMS01617

(Davla et al., 2020)

increased sleep | adult stage | conditional, with speck^JF02142

(Davla et al., 2020)

increased sleep | adult stage | conditional, with speck^UAS.cDa

(Davla et al., 2020)

lethal, with Tdc2^UAS.cCb

(Lee et al., 2021)

paralytic, with kcc^HMS01058

(Rusan et al., 2014)

paralytic | adult stage, with Hsap\SLC6A1^{A288V.UAS.YFP}

(Kasture et al., 2022)

viable, with Acox1^N250S.UAS

(Chung et al., 2020)

Phenotype Manifest In

adult antennal lobe projection neuron | heat sensitive, with TrpA1^UAS.(B).cKa

(Liu et al., 2014)

adult CNS glial cell, with Hsap\GFAP^R79H.UAS

(Wang et al., 2015)

adult s-LNv neuron | adult stage, with Atg5^HMS01244

(Damulewicz et al., 2022)

adult s-LNv neuron | adult stage, with Atg7^JF02787

(Damulewicz et al., 2022)

astrocyte-like glial cell, with Ced-12^KK102788

(Tasdemir-Yilmaz and Freeman, 2014)

astrocyte-like glial cell, with Crk^GD8583

(Tasdemir-Yilmaz and Freeman, 2014)

astrocyte-like glial cell, with drpr^RNAi.UAS

(Tasdemir-Yilmaz and Freeman, 2014)

astrocyte-like glial cell, with EcR^{B1-ΔC655.F645A.UAS}

(Tasdemir-Yilmaz and Freeman, 2014)

astrocyte-like glial cell, with EcR^{B1-ΔC655.W650A.UAS}

(Tasdemir-Yilmaz and Freeman, 2014)

astrocyte-like glial cell, with mbc^GD6965

(Tasdemir-Yilmaz and Freeman, 2014)

astrocyte-like glial cell, with shi^1.UAS

(Tasdemir-Yilmaz and Freeman, 2014)

astrocyte-like glial cell | somatic clone, with EcR^{B1-ΔC655.F645A.UAS}

(Tasdemir-Yilmaz and Freeman, 2014)

astrocyte-like glial cell | somatic clone, with EcR^{B1-ΔC655.W650A.UAS}

(Tasdemir-Yilmaz and Freeman, 2014)

central nervous system, with Pvf2^d02444

(Winkler et al., 2021)

dendrite | larval stage, with CG12926^KK106958

(Yin et al., 2021)

dendrite | larval stage, with CG30392^KK101488

(Yin et al., 2021)

dendrite | larval stage, with GLaz^HMC06329

(Yin et al., 2021)

dendrite | larval stage, with GLaz^KK106377

(Yin et al., 2021)

embryonic/larval brain | larval stage, with CG12926^KK106958

(Yin et al., 2021)

embryonic/larval brain | larval stage, with CG30392^KK101488

(Yin et al., 2021)

embryonic/larval brain | larval stage, with GLaz^HMC06329

(Yin et al., 2021)

embryonic/larval brain | larval stage, with GLaz^KK106377

(Yin et al., 2021)

gamma Kenyon cell, with Ced-12^KK102788

(Tasdemir-Yilmaz and Freeman, 2014)

gamma Kenyon cell, with Crk^GD8583

(Tasdemir-Yilmaz and Freeman, 2014)

gamma Kenyon cell, with drpr^RNAi.UAS

(Tasdemir-Yilmaz and Freeman, 2014)

gamma Kenyon cell, with EcR^{B1-ΔC655.F645A.UAS}

(Tasdemir-Yilmaz and Freeman, 2014)

gamma Kenyon cell, with EcR^{B1-ΔC655.W650A.UAS}

(Tasdemir-Yilmaz and Freeman, 2014)

gamma Kenyon cell, with mbc^GD6965

(Tasdemir-Yilmaz and Freeman, 2014)

gamma Kenyon cell, with shi^1.UAS

(Tasdemir-Yilmaz and Freeman, 2014)

gamma Kenyon cell | somatic clone, with EcR^{B1-ΔC655.F645A.UAS}

(Tasdemir-Yilmaz and Freeman, 2014)

gamma Kenyon cell | somatic clone, with EcR^{B1-ΔC655.W650A.UAS}

(Tasdemir-Yilmaz and Freeman, 2014)

glial cell | heat sensitive, with shi^1.UAS

(Kazama et al., 2011)

LNv neuron | larval stage, with CG12926^KK106958

(Yin et al., 2021)

LNv neuron | larval stage, with CG30392^KK101488

(Yin et al., 2021)

LNv neuron | larval stage, with GLaz^HMC06329

(Yin et al., 2021)

LNv neuron | larval stage, with GLaz^KK106377

(Yin et al., 2021)

plasmatocyte | increased number, with Pvf2^d02444

(Winkler et al., 2021)

synapse, with EcR^{B1-ΔC655.F645A.UAS}

(Tasdemir-Yilmaz and Freeman, 2014)

synapse, with EcR^{B1-ΔC655.W650A.UAS}

(Tasdemir-Yilmaz and Freeman, 2014)

synapse, with shi^1.UAS

(Tasdemir-Yilmaz and Freeman, 2014)

Detailed Description

Statement

Reference

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 28[o]C) of Scer\GAL4^alrm.PD cells in adults significantly reduces the amplitude and slope of excitatory postsynaptic potentials in antennal lobe projection neurons in response to activation of the antennal nerve, compared to controls, with no effect on onset latency.

(Liu et al., 2014)

Expression of hid in Scer\GAL4^alrm.PD expressing neurons (limited to late metamorphosis using Scer\GAL80^ts.αTub84B and shifting pupae to 25[o]) induces astrocyte ablation. A ~50% reduction in astrocyte numbers is seen in the antennal lobe, mushroom body, subesophageal ganglion (SOG) and abdominal segments of the thoracic ganglion (TGab), but the remaining astrocytes are able to infiltrate most of the neuropil with membrane processes. No lethality is observed and the adult flies do not display any obvious behavioral defects. Shifting the pupae to 30[o]C leads to more dramatic astrocyte reduction, with the remaining astrocyte processes appearing sparsely distributed and unable to cover the neuropil space. 41% of pupae are unable to eclose, 32% die during eclosion and only 27% survive as adults. These surviving adults are highly uncoordinated, cannot walk or fly, and die within a few days.

Gross synaptic morphology is unaffected by severe astrocyte ablation. Postsynaptic density length is unchanged and the percentage of synapses with T-bars is unaltered. Synaptic density is reduced in the antennal lobe, mushroom body and superior posterior slope. The astrocyte-ablated tissue is also marked by an increased frequency of ruptured mitochondria. At 84h after pupal formation, synapse numbers are significantly reduced, but several immature synaptic structures are seen. Gross morphology of the adult brain appears unaffected by severe ablated depletion: antennal lobe glomerular organisation appears normal, as are the neuronal projections of second-order antennal lobe projection neurons and pigment dispersing factor (pdf) neurons. The number of projection neurons is comparable in control and astrocyte ablated animals, as are pdf neurons. The membranes of cortex and ensheathing glia in the adult brain exhibit normal morphology.

(Muthukumar et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Scer\GAL4^alrm.PD, drpr^RNAi.UAS has abnormal neuroanatomy phenotype, non-enhanceable by Ced-12^KK102788, Scer\GAL4^alrm.PD

(Tasdemir-Yilmaz and Freeman, 2014)

Ced-12^KK102788, Scer\GAL4^alrm.PD has abnormal neuroanatomy phenotype, non-enhanceable by drpr^RNAi.UAS, Scer\GAL4^alrm.PD

(Tasdemir-Yilmaz and Freeman, 2014)

Suppressed by

Statement

Reference

Scer\GAL4^alrm.PD, Tdc2^UAS.cCb has lethal phenotype, suppressible by Ctet\tetX^TNT-LC.UAS, Scer\GAL4^alrm.PD

(Lee et al., 2021)

Enhancer of

Statement

Reference

Ced-12^KK102788/Scer\GAL4^alrm.PD is an enhancer of abnormal neuroanatomy phenotype of drpr^Δ5

(Tasdemir-Yilmaz and Freeman, 2014)

Crk^GD8583/Scer\GAL4^alrm.PD is an enhancer of abnormal neuroanatomy phenotype of drpr^Δ5

(Tasdemir-Yilmaz and Freeman, 2014)

NOT Enhancer of

Statement

Reference

Ced-12^KK102788, Scer\GAL4^alrm.PD is a non-enhancer of abnormal neuroanatomy phenotype of Scer\GAL4^alrm.PD, drpr^RNAi.UAS

(Tasdemir-Yilmaz and Freeman, 2014)

drpr^RNAi.UAS, Scer\GAL4^alrm.PD is a non-enhancer of abnormal neuroanatomy phenotype of Ced-12^KK102788, Scer\GAL4^alrm.PD

(Tasdemir-Yilmaz and Freeman, 2014)

Suppressor of

Statement

Reference

Hsap\SLC1A3^UAS.cWa/Scer\GAL4^alrm.PD is a suppressor of abnormal locomotor behavior | larval stage phenotype of Eaat1^SM2

(Wu et al., 2022)

Hsap\SLC1A3^C186S.UAS/Scer\GAL4^alrm.PD is a suppressor of abnormal locomotor behavior | larval stage phenotype of Eaat1^SM2

(Wu et al., 2022)

Scer\GAL4^alrm.PD/Hsap\SLC1A3^A329T.UAS is a suppressor | partially of abnormal locomotor behavior | larval stage phenotype of Eaat1^SM2

(Wu et al., 2022)

Hsap\SLC1A3^T318A.UAS/Scer\GAL4^alrm.PD is a suppressor of abnormal locomotor behavior | larval stage phenotype of Eaat1^SM2

(Wu et al., 2022)

Ctet\tetX^TNT-LC.UAS, Scer\GAL4^alrm.PD is a suppressor of lethal phenotype of Scer\GAL4^alrm.PD, Tdc2^UAS.cCb

(Lee et al., 2021)

GABA-B-R2^GD699/Scer\GAL4^alrm.PD is a suppressor of abnormal stress response phenotype of eas²

(Muthukumar et al., 2014)

Bper\ptxA^act.UAS/Scer\GAL4^alrm.PD is a suppressor of abnormal stress response phenotype of eas²

(Muthukumar et al., 2014)

NOT Suppressor of

Statement

Reference

Hsap\SLC1A3^V393I.UAS/Scer\GAL4^alrm.PD is a non-suppressor of abnormal locomotor behavior | larval stage phenotype of Eaat1^SM2

(Wu et al., 2022)

Hsap\SLC1A3^M128R.UAS/Scer\GAL4^alrm.PD is a non-suppressor of abnormal locomotor behavior | larval stage phenotype of Eaat1^SM2

(Wu et al., 2022)

Hsap\SLC1A3^P392V.UAS/Scer\GAL4^alrm.PD is a non-suppressor of abnormal locomotor behavior | larval stage phenotype of Eaat1^SM2

(Wu et al., 2022)

Scer\GAL4^alrm.PD/Hsap\SLC1A3^K114L.UAS is a non-suppressor of abnormal locomotor behavior | larval stage phenotype of Eaat1^SM2

(Wu et al., 2022)

Hsap\SLC1A3^P98G.UAS/Scer\GAL4^alrm.PD is a non-suppressor of abnormal locomotor behavior | larval stage phenotype of Eaat1^SM2

(Wu et al., 2022)

Hsap\SLC1A3^S103V.UAS/Scer\GAL4^alrm.PD is a non-suppressor of abnormal locomotor behavior | larval stage phenotype of Eaat1^SM2

(Wu et al., 2022)

BacA\p35^UAS.cUa/Scer\GAL4^alrm.PD is a non-suppressor of abnormal neuroanatomy | embryonic stage phenotype of htl^AB42

(Stork et al., 2014)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Ced-12^KK102788, Scer\GAL4^alrm.PD has astrocyte-like glial cell phenotype, non-enhanceable by drpr^RNAi.UAS, Scer\GAL4^alrm.PD

(Tasdemir-Yilmaz and Freeman, 2014)

Ced-12^KK102788, Scer\GAL4^alrm.PD has gamma Kenyon cell phenotype, non-enhanceable by drpr^RNAi.UAS, Scer\GAL4^alrm.PD

(Tasdemir-Yilmaz and Freeman, 2014)

Scer\GAL4^alrm.PD, drpr^RNAi.UAS has astrocyte-like glial cell phenotype, non-enhanceable by Ced-12^KK102788, Scer\GAL4^alrm.PD

(Tasdemir-Yilmaz and Freeman, 2014)

Scer\GAL4^alrm.PD, drpr^RNAi.UAS has gamma Kenyon cell phenotype, non-enhanceable by Ced-12^KK102788, Scer\GAL4^alrm.PD

(Tasdemir-Yilmaz and Freeman, 2014)

Enhancer of

Statement

Reference

Ced-12^KK102788/Scer\GAL4^alrm.PD is an enhancer of astrocyte-like glial cell phenotype of drpr^Δ5

(Tasdemir-Yilmaz and Freeman, 2014)

Ced-12^KK102788/Scer\GAL4^alrm.PD is an enhancer of gamma Kenyon cell phenotype of drpr^Δ5

(Tasdemir-Yilmaz and Freeman, 2014)

Crk^GD8583/Scer\GAL4^alrm.PD is an enhancer of astrocyte-like glial cell phenotype of drpr^Δ5

(Tasdemir-Yilmaz and Freeman, 2014)

Crk^GD8583/Scer\GAL4^alrm.PD is an enhancer of gamma Kenyon cell phenotype of drpr^Δ5

(Tasdemir-Yilmaz and Freeman, 2014)

NOT Enhancer of

Statement

Reference

drpr^RNAi.UAS, Scer\GAL4^alrm.PD is a non-enhancer of astrocyte-like glial cell phenotype of Ced-12^KK102788, Scer\GAL4^alrm.PD

(Tasdemir-Yilmaz and Freeman, 2014)

drpr^RNAi.UAS, Scer\GAL4^alrm.PD is a non-enhancer of gamma Kenyon cell phenotype of Ced-12^KK102788, Scer\GAL4^alrm.PD

(Tasdemir-Yilmaz and Freeman, 2014)

Ced-12^KK102788, Scer\GAL4^alrm.PD is a non-enhancer of astrocyte-like glial cell phenotype of Scer\GAL4^alrm.PD, drpr^RNAi.UAS

(Tasdemir-Yilmaz and Freeman, 2014)

Ced-12^KK102788, Scer\GAL4^alrm.PD is a non-enhancer of gamma Kenyon cell phenotype of Scer\GAL4^alrm.PD, drpr^RNAi.UAS

(Tasdemir-Yilmaz and Freeman, 2014)

Suppressor of

Statement

Reference

Eaat1^KK100187/Scer\GAL4^alrm.PD is a suppressor of embryonic/larval astrocyte-like glial cell | conditional phenotype of Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.13XLexAop2.Venus}, Ecol\lexA^GMR50G08

(MacNamee et al., 2016)

NOT Suppressor of

Statement

Reference

BacA\p35^UAS.cUa/Scer\GAL4^alrm.PD is a non-suppressor of astrocyte-like glial cell | embryonic stage phenotype of htl^AB42

(Stork et al., 2014)

BacA\p35^UAS.cUa/Scer\GAL4^alrm.PD is a non-suppressor of glial cell projection | embryonic stage phenotype of htl^AB42

(Stork et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

Gat^UAS.cSa/Scer\GAL4^alrm.PD partially rescues Gat^33-1

(Chaturvedi et al., 2022)

Cpes^UAS.Tag:V5/Scer\GAL4^alrm.PD partially rescues Cpes¹

(Chen et al., 2022)

Eaat1^UAS.Venus/Scer\GAL4^alrm.PD partially rescues Eaat1^hypo

(Peng et al., 2019)

htl^UAS.cMa/Scer\GAL4^alrm.PD partially rescues htl^AB42

(Stork et al., 2014)

htl^λ.UAS/Scer\GAL4^alrm.PD partially rescues htl^AB42

(Stork et al., 2014)

Fails to rescue

orion^A.UAS/Scer\GAL4^alrm.PD fails to rescue orion¹

(Boulanger et al., 2021)

orion^B.UAS/Scer\GAL4^alrm.PD fails to rescue orion¹

(Boulanger et al., 2021)

Cpes^UAS.cKa/Scer\GAL4^alrm.PD fails to rescue Cpes^KO

(Kunduri et al., 2018)

zyd^UAS.cMa/Scer\GAL4^alrm.PD fails to rescue zyd¹

(Melom and Littleton, 2013)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

67032

w^*; wg^Sp-1/CyO; P{alrm-GAL4.D}2

67031

w^*; P{alrm-GAL4.D}3/CyO; Dr¹/TM3, Sb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Scer\GAL4^alrm.PD

Name Synonyms

astrocytic leucine-rich repeat molecule promoter construct of Doherty

Secondary FlyBase IDs

References (99)

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