dar13010 contains a premature stop codon so that only a fragment of the N-terminal half of the protein can be produced, without the nuclear localization signal and zinc finger domains.
In dar13010 mutants, major dendrites of class IV da neurons are either missing or shortened, consequently resulting in a reduced dendritic arbor. In contrast, the axonal growth of these neurons is normal. Dendrites of class I da neurons are also severely shortened.
dar13232/dar13010 mutants, generated by crossing dar13232 germline clones with dar13010 mutants show the same phenotype as dar13010 zygotic mutants, that is, the dendrites are reduced but the axons are normal. Dendritic defects are visible as early as 16-17 hours after egg laying and become more pronounced in 18-19 hours after egg laying, suggesting these mutants exhibit reduced outgrowth of dendrites.
Single neurons homozygous for dar13010, generated through MARCM, exhibit severely reduced dendritic arbors, supporting the notion that dar1 functions cell-autonomously. Dendrites are dramatically reduced in all four classes of da neurons (class I-ddaD and ddaE, class II-ddaB, class III-ddaA, and class IV-ddaC). In contrast, there is no significant defect in axonal growth in these mutants.
dar13010 has abnormal neuroanatomy | somatic clone phenotype, suppressible | partially by Scer\GAL4unspecified/nudEUAS.A
dar13010 has abnormal neuroanatomy phenotype, non-suppressible by knUAS.cMa/Scer\GAL421-7
dar13010 is a suppressor of abnormal neuroanatomy | somatic clone phenotype of Scer\GAL421-7, knUAS.cMa
dar13010 is a non-suppressor of abnormal neuroanatomy phenotype of Rac1UAS.cLa, Scer\GAL4477
dar13010 has dendritic arborizing neuron | somatic clone phenotype, suppressible | partially by Scer\GAL4unspecified/nudEUAS.A
dar13010 has dendritic arborizing neuron phenotype, non-suppressible by knUAS.cMa/Scer\GAL421-7
dar13010 is a suppressor of dendritic arborizing neuron | somatic clone phenotype of Scer\GAL421-7, knUAS.cMa
dar13010 is a non-suppressor of dendritic arborizing neuron phenotype of Rac1UAS.cLa, Scer\GAL4477
Expression of nudEScer\UAS.A under the control of Scer\GAL4unspecified partially rescues the increased number of bipolar dendritic arborizing neurons seen in dar13010/dar13010 mutant clones.
A dar13010 mutant background suppresses the increase in dendrite length found upon expression of knScer\UAS.cMa in class I da neurons under the control of Scer\GAL421-7 and results in a severe reduction in dendrite length.
A dar13010 mutant background does not block the dendrite branching activity found upon expression of Rac1Scer\UAS.cLa under the control of Scer\GAL4477.