UAS regulatory sequences drive expression of a short inverted repeat.
Expressing ctHMS00924 under the control of Scer\GAL4ppk.PU results in a significantly reduced number of branch points in the dendrites of class IV dendritic arborizing neurons in third instar larvae compared to wild-type controls.
Adult escapers expressing ctHMS00924 under the control of Scer\GAL4esg-NP5130 show ureters that are short and lack the lower region, have increased numbers of progenitor cells and have decreased numbers of principal cells, which disperse throughout the ureter and lower tubule, as compared to controls; renal tubule clones expressing ctHMS00924 under the control of Scer\GAL4esg-NP5130 display ectopic enteroendocrine-like cells (i.e. Pros-, Rab3-, Brp- and Ast A-positive, diploid cells) along the tubules, as compared to controls.
Expression of ctHMS00924 under the control of Scer\GAL4ppk.1.9 results in strong and penetrant phenotypes in C-IV neurons but no phenotypes in C-I neurons. DdaC, v'ada and vdaB neurons all have reduced total dendritic length compared to controls. The total number of dendritic branches is also reduced in ddaC and v'ada and vdaB neurons.
Scer\GAL4NP5130, ctHMS00924 has increased cell number | somatic clone phenotype, enhanceable by NVDRC.cUa, Scer\GAL4NP5130
Scer\GAL4Mef2.PR, ctHMS00924 has flightless phenotype, non-suppressible by salmKK106735/Scer\GAL4Mef2.PR
ctHMS00924, Scer\GAL4NP5130 is a non-enhancer of decreased cell number | somatic clone phenotype of NVDRC.cUa, Scer\GAL4NP5130
ctHMS00924, Scer\GAL4NP5130 is a suppressor of decreased cell number | somatic clone phenotype of NUAS.Tag:V5,Tag:polyHis, Scer\GAL4NP5130
ctHMS00924/Scer\GAL4Mef2.PR is a non-suppressor of flightless phenotype of Scer\GAL4Mef2.PR, salmKK106735
ctHMS00924, Scer\GAL4NP5130 is a non-suppressor of increased cell number | somatic clone phenotype of NVDRC.cUa, Scer\GAL4NP5130
NUAS.Tag:V5,Tag:polyHis, Scer\GAL4NP5130, ctHMS00924 has increased cell number phenotype
Scer\GAL4NP5130, ctHMS00924 has adult ureter | somatic clone phenotype, enhanceable by NVDRC.cUa, Scer\GAL4NP5130
Scer\GAL4NP5130, ctHMS00924 has adult enteroendocrine cell | ectopic | somatic clone phenotype, enhanceable by NVDRC.cUa, Scer\GAL4NP5130
ctHMS00924, Scer\GAL4NP5130 is a non-enhancer of midgut | somatic clone phenotype of NVDRC.cUa, Scer\GAL4NP5130
ctHMS00924/Scer\GAL4Mef2.PR is a suppressor of indirect flight muscle | adult stage phenotype of Scer\GAL4Mef2.PR, salmKK106735
ctHMS00924/Scer\GAL4Mef2.PR is a suppressor of mitochondrion | adult stage phenotype of Scer\GAL4Mef2.PR, salmKK106735
ctHMS00924, Scer\GAL4NP5130 is a suppressor of Malpighian tubule | somatic clone phenotype of NUAS.Tag:V5,Tag:polyHis, Scer\GAL4NP5130
ctHMS00924, Scer\GAL4NP5130 is a non-suppressor of midgut | somatic clone phenotype of NVDRC.cUa, Scer\GAL4NP5130
ctHMS00924, Scer\GAL4NP5130 is a non-suppressor of enteroendocrine cell | somatic clone phenotype of NVDRC.cUa, Scer\GAL4NP5130
NUAS.Tag:V5,Tag:polyHis, Scer\GAL4NP5130, ctHMS00924 has Malpighian tubule phenotype
NUAS.Tag:V5,Tag:polyHis, Scer\GAL4NP5130, ctHMS00924 has enterocyte | ectopic phenotype
The co-expression of ctHMS00924 and NUAS.Tag:V5,Tag:polyHis under the control of Scer\GAL4esg-NP5130 leads to the abnormal differentiation of renal tubule progenitors into enterocyte-like cells (i.e. Pdm1-positive cells).