FB2024_03 , released June 25, 2024
Allele: Scer\GAL4GMR15A04
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General Information
Symbol
Scer\GAL4GMR15A04
Species
S. cerevisiae
Name
FlyBase ID
FBal0249987
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
Carried in Construct
P{GMR15A04-GAL4}
Also Known As
R15A04-GAL4, R15A04
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Pfeiffer et al., 2011.2.9)
Progenitor genotype
Carried in construct
P{GMR15A04-GAL4}
Cytology
Description

A 3346 base pair fragment from the flanking non-coding or intronic region of sc is fused upstream of a Drosophila core synthetic promoter (DSCP) that is followed by sequence encoding a GAL4 driver.

(Pfeiffer et al., 2011.2.9)
Allele components
Component
Use(s)
Regulatory region(s)
GMR15A04
Encoded product / tool
GAL4
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      prow | intense | restricted
      (Jenett and Svirskas, 2012.9.24)
      flange | faint | restricted
      (Jenett and Svirskas, 2012.9.24)
      adult crepine | faint | restricted
      (Jenett and Svirskas, 2012.9.24)
      bulb | faint | restricted
      (Jenett and Svirskas, 2012.9.24)
      adult mushroom body | intense | restricted
      (Jenett and Svirskas, 2012.9.24)
      superior medial protocerebrum | restricted
      (Jenett and Svirskas, 2012.9.24)
      mushroom body alpha lobe slice 1
      (Huetteroth et al., 2015, Yamagata et al., 2015)
      mushroom body beta' lobe slice 1
      (Huetteroth et al., 2015, Yamagata et al., 2015)
      mushroom body beta lobe slice 2
      (Huetteroth et al., 2015, Yamagata et al., 2015)
      mushroom body gamma lobe slice 5
      (Huetteroth et al., 2015, Yamagata et al., 2015)
      dopaminergic PAM neuron 11
      (Huetteroth et al., 2015)
      dopaminergic PAM neuron 13
      (Huetteroth et al., 2015)
      dopaminergic PAM neuron 14
      (Huetteroth et al., 2015)
      dopaminergic PAM neuron 4
      (Huetteroth et al., 2015)
      dopaminergic PAM neuron 1
      (Huetteroth et al., 2015)
      mushroom body pedunculus
      (Yamagata et al., 2015)
      dopaminergic PAM neuron | subset
      (Yamagata et al., 2015)
      adult brain | restricted
      (Yamagata et al., 2015)
      Additional Information
      Statement
      Reference

      ScerGAL4GMR15A04 drives expression in ~26 tyrosine hydroxylase positive dopaminergic PAM neurons and ~4 tyrosine hydroxylase negative, non dopaminergic neurons.

      (Huetteroth et al., 2015)

      ScerGAL4GMR15A04 drives expression in a subset of PAM neurons that innervate mushroom body beta lobe slice 2, alpha lobe slice 1, beta' lobe slice 1, gamma lobe slice 5 and the pedunculus.

      (Yamagata et al., 2015)
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Blocking the output (using shi1.UAS at 33[o]C) of Scer\GAL4GMR15A04 neurons shortly before and during training significantly impairs long-term memory, but not short-term memory in starved flies trained with sucrose, compared to controls.

      Acute thermogenetic activation (using TrpA1UAS.(B).cKa at 33[o]C) of Scer\GAL4GMR15A04 neurons results in a significant long-term preference for a paired odor (artificial memory) in starved flies, but not flies that are fed between training and testing, compared to controls.

      (Huetteroth et al., 2015)

      Thermogenetic activation (using TrpA1UAS.(B).cKa at 30[o]C) of Scer\GAL4GMR15A04 cells in adults, in combination with a paired odor, results in a significant increase in long-term, but not short-term, associative attractive olfactory memory, compared to controls; memory induction does not occur when activation is restricted to a smaller subset of cells using Scer\GAL80R58E02.

      Inhibition (using shits1.IVS.p10.20xUAS at 33[o]C) of Scer\GAL4GMR15A04 cells in adults during training significantly reduces long-term, but not short-term, associative appetitive (sugar) olfactory memory, compared to controls; memory reduction does not occur when activation is restricted to a smaller subset of cells using Scer\GAL80R58E02.

      (Yamagata et al., 2015)

      Optogenetic activation (using Crei\ChR2UAS.cSa with 470 nm (blue) light) of Scer\GAL4GMR15A04 cells in larvae results in a significant increase in turning behaviors, compared to controls.

      (Vogelstein et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Suppressor of
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Scer\GAL4GMR15A04
      Name Synonyms
      Secondary FlyBase IDs
        References (12)