FB2024_03 , released June 25, 2024
Allele: Scer\GAL4GMR11H09
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General Information
Symbol
Scer\GAL4GMR11H09
Species
S. cerevisiae
Name
FlyBase ID
FBal0249705
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
Carried in Construct
P{GMR11H09-GAL4}
Also Known As
R11H09-Gal4
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Pfeiffer et al., 2011.2.9)
Progenitor genotype
Carried in construct
P{GMR11H09-GAL4}
Cytology
Description

A 591 base pair fragment from the flanking non-coding or intronic region of Fas2 is fused upstream of a Drosophila core synthetic promoter (DSCP) that is followed by sequence encoding a GAL4 driver.

(Pfeiffer et al., 2011.2.9)
Allele components
Component
Use(s)
Regulatory region(s)
GMR11H09
Encoded product / tool
GAL4
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      adult taste projection neuron 2
      (Dolan et al., 2019)
      adult taste projection neuron 3 | subset
      (Kim et al., 2017)
      adult antennal lobe local neuron | subset
      (Kim et al., 2017)
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Optogenetic activation (using Cnoc\ChR1Cs.IVS.20xUAS.Venus with 635 nm (red) light) of Scer\GAL4GMR11H09 neurons does not significantly increase proboscis extension reflex (PER) in the absense of nutritional stimuli, compared to controls; PER in response to 100 mM sucrose presentation is significantly reduced, compared to controls; this aversion is sustained for several minutes after activation has finished.

      Inhibition (using shi1.UAS at 30[o]C) of Scer\GAL4GMR11H09 neurons does not affect PER in response to 100 mM sucrose presentation, but does significantly increase PER in response to presentation of a sweet and bitter mixture compared to controls.

      Inhibition (using shi1.UAS at 30[o]C) of Scer\GAL4GMR11H09 neurons significantly suppresses the reduction in PER response to presentation of 100 mM sucrose seen during and after quinine application to the proboscis, compared to controls; this effect is also seen when silencing occurs only during, but not only after, quinine application.

      (Kim et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Scer\GAL4GMR11H09
      Name Synonyms
      Secondary FlyBase IDs
        References (5)