FlyBase Allele Report: Hsap\SOD1[UAS.cWa]

General Information

Symbol

Hsap\SOD1^UAS.cWa

Species

H. sapiens

Name

Saccharomyces cerevisiae UAS construct a of Watson

FlyBase ID

FBal0248884

Feature type

allele

Associated gene

Hsap\SOD1

Associated Insertion(s)

Carried in Construct

P{UAS-hSOD1}

Also Known As

UAS-hSOD1, UAS-SOD1

Key Links

Transgenic product class

cDNA

(Watson et al., 2008)

wild_type

(Watson et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Watson et al., 2008)

wild_type

(Watson et al., 2008)

Mutagen

in vitro construct

(Watson et al., 2008)

Progenitor genotype

P{UAS-Hsap\SOD1.P}

(Watson et al., 2008)

Carried in construct

P{UAS-hSOD1}

Cytology

Description

Carries a corrected K75R amino acid substitution to match the canonical Hsap\SOD1 open reading frame.

(Watson et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Hsap\SOD1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of amyotrophic lateral sclerosis

CEA

(Watson et al., 2008)

model of amyotrophic lateral sclerosis type 1

CEA

(De Rose et al., 2017)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates tauopathy

modeled by Hsap\MAPT^R406W.UAS

(Huang et al., 2015)

ameliorates episodic ataxia type 6

modeled by Eaat1^hypo

(Peng et al., 2019)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior, with Scer\GAL4^Toll-6-D42

(Watson et al., 2008)

abnormal neurophysiology, with Scer\GAL4^Toll-6-D42

(Watson et al., 2008)

Phenotype Manifest In

Detailed Description

Statement

Reference

Animals expressing Hsap\SOD1^Scer\UAS.cWa in motor neurons under the control of Scer\GAL4^D42 perform similarly to wild-type in larval turning assays.

(Joardar et al., 2015)

park²⁵ mutant flies expressing Hsap\SOD1^Scer\UAS.cWa under the control of Scer\GAL4^twi.PG do not show a difference in survival compared to park²⁵ flies, up to 4 days of age. However, at 11 days of age only 10% of the park²⁵ flies remain, compared to 80% of the park²⁵ flies expressing Hsap\SOD1^Scer\UAS.cWa under the control of Scer\GAL4^twi.PG. None of the park²⁵ flies survive to 17 days of age, at which time point approximately 80% of Hsap\SOD1^Scer\UAS.cWa-expressing flies are still alive.

(Saini et al., 2010)

Flies overexpressing Hsap\SOD1^Scer\UAS.cWa driven by Scer\GAL4^D42 in motor neurons show a progressive loss of climbing ability starting at 21 days after eclosion.

Flies overexpressing Hsap\SOD1^Scer\UAS.cWa driven by Scer\GAL4^D42 show no detectable loss of neuronal nuclei over time.

No anatomical degeneration of the retina occur in flies overexpressing Hsap\SOD1^Scer\UAS.cWa driven by Scer\GAL4^GMR.PF.

Synaptic transmission along the giant fibre motor pathway is abnormal in flies overexpressing Hsap\SOD1^Scer\UAS.cWa driven by Scer\GAL4^D42 in motor neurons.

(Watson et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Hsap\SOD1^UAS.cWa, Scer\GAL4^GMR.PU is an enhancer of visible phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^GMR.PU

(Huang et al., 2015)

Hsap\SOD1^UAS.cWa, Scer\GAL4^elav.PU is an enhancer of abnormal locomotor behavior phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PU

(Huang et al., 2015)

Hsap\SOD1^UAS.cWa, Scer\GAL4^elav.PU is an enhancer of abnormal neuroanatomy phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PU

(Huang et al., 2015)

Suppressor of

Statement

Reference

Hsap\SOD1^UAS.cWa/Scer\GAL4^nSyb.PS is a suppressor of abnormal neurophysiology | larval stage phenotype of Eaat1^hypo

(Peng et al., 2019)

Hsap\SOD1^UAS.cWa/Scer\GAL4^nSyb.PS is a suppressor of abnormal locomotor behavior | larval stage phenotype of Eaat1^hypo

(Peng et al., 2019)

Hsap\SOD1^UAS.cWa/Scer\GAL4^ChAT.7.4 is a suppressor of abnormal neurophysiology | larval stage phenotype of Eaat1^hypo

(Peng et al., 2019)

Hsap\SOD1^UAS.cWa/Scer\GAL4^ChAT.7.4 is a suppressor of abnormal locomotor behavior | larval stage phenotype of Eaat1^hypo

(Peng et al., 2019)

Hsap\SOD1^UAS.cWa/Scer\GAL4^C57 is a suppressor of abnormal neurophysiology | larval stage phenotype of Eaat1^hypo

(Peng et al., 2019)

Hsap\SOD1^UAS.cWa/Scer\GAL4^C57 is a suppressor | partially of abnormal locomotor behavior | larval stage phenotype of Eaat1^hypo

(Peng et al., 2019)

Hsap\SOD1^UAS.cWa/Scer\GAL4^ChAT.7.4 is a suppressor of abnormal neuroanatomy | larval stage phenotype of Eaat1^hypo

(Peng et al., 2019)

Hsap\SOD1^UAS.cWa/Scer\GAL4^C57 is a suppressor of abnormal neuroanatomy | larval stage phenotype of Eaat1^hypo

(Peng et al., 2019)

Hsap\SOD1^UAS.cWa/Scer\GAL4^nSyb.PS is a suppressor of abnormal neuroanatomy | larval stage phenotype of Eaat1^hypo

(Peng et al., 2019)

Hsap\SOD1^UAS.cWa/Scer\GAL4^VGlut-OK371 is a suppressor of abnormal neuroanatomy | larval stage phenotype of Eaat1^hypo

(Peng et al., 2019)

Hsap\SOD1^UAS.cWa/Scer\GAL4^Toll-6-D42 is a suppressor of abnormal neuroanatomy | larval stage phenotype of Eaat1^hypo

(Peng et al., 2019)

Hsap\SOD1^UAS.cWa/Scer\GAL4^twi.PG is a suppressor of short lived | chemical conditional phenotype of park²⁵

(Saini et al., 2010)

Hsap\SOD1^UAS.cWa/Scer\GAL4^twi.PG is a suppressor of long lived | recessive | chemical conditional phenotype of park²⁵

(Saini et al., 2010)

NOT Suppressor of

Statement

Reference

Hsap\SOD1^UAS.cWa, Scer\GAL4^ppk.PU is a non-suppressor of abnormal neuroanatomy | P-stage phenotype of Scer\GAL4^ppk.PU, cnc^GD4437

(Chew et al., 2021)

Hsap\SOD1^UAS.cWa/Scer\GAL4^VGlut-OK371 is a non-suppressor of abnormal neurophysiology | larval stage phenotype of Eaat1^hypo

(Peng et al., 2019)

Hsap\SOD1^UAS.cWa/Scer\GAL4^VGlut-OK371 is a non-suppressor of abnormal locomotor behavior | larval stage phenotype of Eaat1^hypo

(Peng et al., 2019)

Hsap\SOD1^UAS.cWa/Scer\GAL4^Gad1.3.098 is a non-suppressor of abnormal neurophysiology | larval stage phenotype of Eaat1^hypo

(Peng et al., 2019)

Hsap\SOD1^UAS.cWa/Scer\GAL4^Gad1.3.098 is a non-suppressor of abnormal locomotor behavior | larval stage phenotype of Eaat1^hypo

(Peng et al., 2019)

Scer\GAL4^NP2225/Hsap\SOD1^UAS.cWa is a non-suppressor of abnormal neurophysiology | larval stage phenotype of Eaat1^hypo

(Peng et al., 2019)

Scer\GAL4^NP2225/Hsap\SOD1^UAS.cWa is a non-suppressor of abnormal locomotor behavior | larval stage phenotype of Eaat1^hypo

(Peng et al., 2019)

Hsap\SOD1^UAS.cWa, Cat^UAS.cAa, Scer\GAL4^repo is a non-suppressor of abnormal neuroanatomy phenotype of Cpes^KO

(Kunduri et al., 2018)

Other

Statement

Reference

Hsap\MAPT^R406W.UAS, Hsap\SOD1^UAS.cWa, Scer\GAL4^elav.PU has short lived phenotype

(Huang et al., 2015)

Phenotype Manifest In

Enhancer of

Statement

Reference

Hsap\SOD1^UAS.cWa, Scer\GAL4^GMR.PU is an enhancer of eye phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^GMR.PU

(Huang et al., 2015)

Hsap\SOD1^UAS.cWa, Scer\GAL4^elav.PU is an enhancer of brain phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PU

(Huang et al., 2015)

Hsap\SOD1^UAS.cWa, Scer\GAL4^elav.PU is an enhancer of neuron phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PU

(Huang et al., 2015)

Suppressor of

Statement

Reference

Hsap\SOD1^UAS.cWa/Scer\GAL4^ChAT.7.4 is a suppressor of embryonic/larval neuromuscular junction | larval stage phenotype of Eaat1^hypo

(Peng et al., 2019)

Hsap\SOD1^UAS.cWa/Scer\GAL4^ChAT.7.4 is a suppressor of NMJ bouton | increased number | larval stage phenotype of Eaat1^hypo

(Peng et al., 2019)

Hsap\SOD1^UAS.cWa/Scer\GAL4^C57 is a suppressor of embryonic/larval neuromuscular junction | larval stage phenotype of Eaat1^hypo

(Peng et al., 2019)

Hsap\SOD1^UAS.cWa/Scer\GAL4^C57 is a suppressor of NMJ bouton | increased number | larval stage phenotype of Eaat1^hypo

(Peng et al., 2019)

Hsap\SOD1^UAS.cWa/Scer\GAL4^nSyb.PS is a suppressor of embryonic/larval neuromuscular junction | larval stage phenotype of Eaat1^hypo

(Peng et al., 2019)

Hsap\SOD1^UAS.cWa/Scer\GAL4^nSyb.PS is a suppressor of NMJ bouton | increased number | larval stage phenotype of Eaat1^hypo

(Peng et al., 2019)

Hsap\SOD1^UAS.cWa/Scer\GAL4^VGlut-OK371 is a suppressor | partially of embryonic/larval neuromuscular junction | larval stage phenotype of Eaat1^hypo

(Peng et al., 2019)

Hsap\SOD1^UAS.cWa/Scer\GAL4^VGlut-OK371 is a suppressor of NMJ bouton | increased number | larval stage phenotype of Eaat1^hypo

(Peng et al., 2019)

Hsap\SOD1^UAS.cWa/Scer\GAL4^Toll-6-D42 is a suppressor of embryonic/larval neuromuscular junction | larval stage phenotype of Eaat1^hypo

(Peng et al., 2019)

Hsap\SOD1^UAS.cWa/Scer\GAL4^Toll-6-D42 is a suppressor of NMJ bouton | increased number | larval stage phenotype of Eaat1^hypo

(Peng et al., 2019)

Hsap\SOD1^UAS.cWa/Scer\GAL4^elav.PLu is a suppressor of eye | somatic clone phenotype of ifc^KO

(Jung et al., 2017)

Hsap\SOD1^UAS.cWa/Scer\GAL4^elav.PLu is a suppressor of autophagosome | somatic clone | adult stage phenotype of ifc^KO

(Jung et al., 2017)

NOT Suppressor of

Statement

Reference

Hsap\SOD1^UAS.cWa, Scer\GAL4^ppk.PU is a non-suppressor of dendrite | P-stage phenotype of Scer\GAL4^ppk.PU, cnc^GD4437

(Chew et al., 2021)

Hsap\SOD1^UAS.cWa, Scer\GAL4^ppk.PU is a non-suppressor of larval multidendritic class IV neuron | P-stage phenotype of Scer\GAL4^ppk.PU, cnc^GD4437

(Chew et al., 2021)

Scer\GAL4^NP2225/Hsap\SOD1^UAS.cWa is a non-suppressor of embryonic/larval neuromuscular junction | larval stage phenotype of Eaat1^hypo

(Peng et al., 2019)

Hsap\SOD1^UAS.cWa/Scer\GAL4^Gad1.3.098 is a non-suppressor of embryonic/larval neuromuscular junction | larval stage phenotype of Eaat1^hypo

(Peng et al., 2019)

Hsap\SOD1^UAS.cWa, Cat^UAS.cAa, Scer\GAL4^repo is a non-suppressor of adult brain cell body glial cell phenotype of Cpes^KO

(Kunduri et al., 2018)

Hsap\SOD1^UAS.cWa, Cat^UAS.cAa, Scer\GAL4^repo is a non-suppressor of adult brain phenotype of Cpes^KO

(Kunduri et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Co-expression of Hsap\SOD1^Scer\UAS.cWa enhances the toxicity of Scer\GAL4^GMR.PU>Hsap\MAPT^{R406W.Scer\UAS} in Drosophila compound eyes. The rough eye phenotype caused by Hsap\MAPT^{R406W.Scer\UAS}-expression becomes more severe, and the ommatidia are more severely fused and irregular.

Co-expression of Hsap\SOD1^Scer\UAS.cWa shortens the lifespan of files expressing Scer\GAL4^elav.PU>Hsap\MAPT^{R406W.Scer\UAS} and exacerbates their movement impairment.

Co-expression of Hsap\SOD1^Scer\UAS.cWa exacerbates the brain damage caused by the expression of Scer\GAL4^elav.PU>Hsap\MAPT^{R406W.Scer\UAS}. The number of vacuoles is significantly increased in the double-transgenic flies compared with Hsap\MAPT^{R406W.Scer\UAS}-expression alone.

(Huang et al., 2015)

park²⁵ mutant flies expressing Hsap\SOD1^Scer\UAS.cWa under the control of Scer\GAL4^twi.PG do not show a difference in survival compared to park²⁵ flies, up to 4 days of age. However, at 11 days of age only 10% of the park²⁵ flies remain, compared to 80% of the park²⁵ flies expressing Hsap\SOD1^Scer\UAS.cWa under the control of Scer\GAL4^twi.PG. None of the park²⁵ flies sruvvie to 17 days of age, at which time point approximately 80% of Hsap\SOD1^Scer\UAS.cWa-expressing flies are still alive.

(Saini et al., 2010)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

33606

w¹¹¹⁸; P{UAS-hSOD1}16.2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Hsap\SOD1^Scer\UAS.cWa

Hsap\SOD1^UAS.cWa

hSOD1^WT

(Cacciottolo et al., 2019)

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Watson

Secondary FlyBase IDs

References (13)

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