FlyBase Allele Report: Dmel\Fmr1[UAS.YFP]

General Information

Symbol

Dmel\Fmr1^UAS.YFP

Species

D. melanogaster

Name

FlyBase ID

FBal0248554

Feature type

allele

Associated gene

Dmel\Fmr1

Associated Insertion(s)

Carried in Construct

P{UAS-Fmr1.YFP}

Key Links

Transgenic product class

wild_type

(Cziko et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Cziko et al., 2009)

Mutagen

in vitro construct

(Cziko et al., 2009)

Progenitor genotype

Carried in construct

P{UAS-Fmr1.YFP}

Cytology

Description

UAS regulatory sequences drive expression of Fmr1 tagged with YFP.

(Cziko et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Fmr1

Tagged with

YFP

yellow fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates amyotrophic lateral sclerosis

modeled by Hsap\TARDBP^UAS.YFP.cEa

(Coyne et al., 2015)

modeled by Hsap\TARDBP^{G298S.UAS.YFP}

(Coyne et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^Toll-6-D42

(Coyne et al., 2015)

Phenotype Manifest In

NMJ bouton | third instar larval stage, with Scer\GAL4^Toll-6-D42

(Coyne et al., 2015)

Detailed Description

Statement

Reference

Ectopic expression of Fmr1^{Scer\UAS.T:Avic\GFP-YFP} under the control of Scer\GAL4^Toll-6-D42 results in significantly decreased density of futsch positive loops on synaptic boutons on neuromuscular junctions of third instar larvae compared to controls.

(Coyne et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Fmr1^UAS.YFP, Scer\GAL4^Toll-6-D42 is an enhancer of abnormal locomotor behavior | third instar larval stage phenotype of Scer\GAL4^Toll-6-D42, TBPH^GD6943

(Coyne et al., 2015)

Suppressor of

Statement

Reference

Fmr1^UAS.YFP, Scer\GAL4^GMR.PU is a suppressor of abnormal eye color | adult stage phenotype of Hsap\TARDBP^UAS.YFP.cEa, Scer\GAL4^GMR.PU

(Coyne et al., 2015)

Fmr1^UAS.YFP, Scer\GAL4^Toll-6-D42 is a suppressor of short lived phenotype of Hsap\TARDBP^{G298S.UAS.YFP}, Scer\GAL4^Toll-6-D42

(Coyne et al., 2015)

Fmr1^UAS.YFP, Scer\GAL4^Toll-6-D42 is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Hsap\TARDBP^{G298S.UAS.YFP}, Scer\GAL4^Toll-6-D42

(Coyne et al., 2015)

Fmr1^UAS.YFP, Scer\GAL4^GMR.PU is a suppressor of abnormal eye color | adult stage phenotype of Hsap\TARDBP^{G298S.UAS.YFP}, Scer\GAL4^GMR.PU

(Coyne et al., 2015)

Fmr1^UAS.YFP, Scer\GAL4^Toll-6-D42 is a suppressor of abnormal locomotor behavior | third instar larval stage phenotype of Hsap\TARDBP^UAS.YFP.cEa, Scer\GAL4^Toll-6-D42

(Coyne et al., 2015)

Fmr1^UAS.YFP, Scer\GAL4^Toll-6-D42 is a suppressor of short lived phenotype of Hsap\TARDBP^UAS.YFP.cEa, Scer\GAL4^Toll-6-D42

(Coyne et al., 2015)

Fmr1^UAS.YFP, Scer\GAL4^Toll-6-D42 is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Hsap\TARDBP^UAS.YFP.cEa, Scer\GAL4^Toll-6-D42

(Coyne et al., 2015)

Fmr1^UAS.YFP, Scer\GAL4^Toll-6-D42 is a suppressor of abnormal locomotor behavior | third instar larval stage phenotype of Hsap\TARDBP^{G298S.UAS.YFP}, Scer\GAL4^Toll-6-D42

(Coyne et al., 2015)

Phenotype Manifest In

Suppressor of

Statement

Reference

Fmr1^UAS.YFP, Scer\GAL4^GMR.PU is a suppressor of eye | adult stage phenotype of Hsap\TARDBP^UAS.YFP.cEa, Scer\GAL4^GMR.PU

(Coyne et al., 2015)

Fmr1^UAS.YFP, Scer\GAL4^GMR.PU is a suppressor of eye | adult stage phenotype of Hsap\TARDBP^{G298S.UAS.YFP}, Scer\GAL4^GMR.PU

(Coyne et al., 2015)

Fmr1^UAS.YFP, Scer\GAL4^Toll-6-D42 is a suppressor of NMJ bouton | third instar larval stage phenotype of Hsap\TARDBP^UAS.YFP.cEa, Scer\GAL4^Toll-6-D42

(Coyne et al., 2015)

Fmr1^UAS.YFP, Scer\GAL4^Toll-6-D42 is a suppressor of NMJ bouton | third instar larval stage phenotype of Hsap\TARDBP^{G298S.UAS.YFP}, Scer\GAL4^Toll-6-D42

(Coyne et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The longer time it takes for third instar larvae expressing TBPH^GD6943 under the control of Scer\GAL4^Toll-6-D42 to roll over in a turning assay compared to wild-type controls is lengthened further by co-expression of Fmr1^{Scer\UAS.T:Avic\GFP-YFP}.

(Coyne et al., 2015)

Xenogenetic Interactions

Statement

Reference

The depigmentation phenotype in the adult eye and the lengthened time it takes for larvae to roll over in a turning assay characteristic for animals expressing Hsap\TARDBP^{Scer\UAS.T:Avic\GFP-YFP.cEa} under the control of Scer\GAL4^GMR.PU or Scer\GAL4^Toll-6-D42 respectively, are restored by co-expression of Fmr1^{Scer\UAS.T:Avic\GFP-YFP}.

The short adult life span, the increased number of satellite boutons along with the reduction in the density of futsch positive loops on synaptic boutons on neuromuscular junctions of third instar larvae characteristic for animals expressing Hsap\TARDBP^{Scer\UAS.T:Avic\GFP-YFP.cEa} under the control of Scer\GAL4^Toll-6-D42 can be fully (satellite boutons) or partially (loops density on synaptic boutons, adult life span) rescued by co-expression of Fmr1^{Scer\UAS.T:Avic\GFP-YFP}.

The depigmentation phenotype in the adult eye and the lengthened time it takes for larvae to roll over in a turning assay characteristic for animals expressing Hsap\TARDBP^{G298S.Scer\UAS.T:Avic\GFP-YFP} under the control of Scer\GAL4^GMR.PU or Scer\GAL4^Toll-6-D42 respectively, are restored by co-expression of Fmr1^{Scer\UAS.T:Avic\GFP-YFP}.

The short adult life span, the increased number of satellite boutons along with the reduction in the density of futsch positive loops on synaptic boutons on neuromuscular junctions of third instar larvae characteristic for animals expressing Hsap\TARDBP^{G298S.Scer\UAS.T:Avic\GFP-YFP} under the control of Scer\GAL4^Toll-6-D42 can be fully (satellite boutons) or partially (loops density on synaptic boutons, adult life span) rescued by co-expression of Fmr1^{Scer\UAS.T:Avic\GFP-YFP}.

(Coyne et al., 2015)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Fmr1^{Scer\UAS.T:Avic\GFP-YFP}

Fmr1^UAS.YFP

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

Open Close