FB2024_03 , released June 25, 2024
Allele: Mmus\PrnpP101L.UAS.Tag:3F4
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General Information
Symbol
Mmus\PrnpP101L.UAS.Tag:3F4
Species
M. musculus
Name
FlyBase ID
FBal0247618
Feature type
allele
Associated gene
Mmus\Prnp
Associated Insertion(s)
Carried in Construct
P{UAS-Prnp.P101L}
Transgenic product class
missense_variant
(Choi et al., 2010)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Choi et al., 2010)
Mutagen
in vitro construct
(Choi et al., 2010)
Progenitor genotype
Carried in construct
P{UAS-Prnp.P101L}
Cytology
Description

UASt regulatory sequences drive expression of the open reading frame of Mmus\Prnp with a Pro to Leu substitution at residue 101 and tagged with Tag:3F4 at residues 109M-112M..

(Choi et al., 2010)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Mmus\Prnp
Tagged with
Tag:3F4
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  Gerstmann-Straussler-Scheinker syndrome
      CEA
      (Choi et al., 2010)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Flies expressing Mmus\PrnpP101L.Scer\UAS.T:Hsap\PRNP-3F4 (ubiquitously, under the control of Scer\GAL4αTub84B.PL) increases susceptibility to DTT. After exposure to 25mM DTT for 144 hours, more than 70% of control flies are alive, whereas less than 50% of Mmus\PrnpP101L.Scer\UAS.T:Hsap\PRNP-3F4 flies survive. These flies also show significantly increased resistance to H[[2]]O[[2]] compared to controls. After treatment with 1% H[[2]]O[[2]], more than 70% of Mmus\PrnpP101L.Scer\UAS.T:Hsap\PRNP-3F4 flies survive, while less than 60% of control flies remain. Conversely, sensitivity to paraquat is significantly increased in Mmus\PrnpP101L.Scer\UAS.T:Hsap\PRNP-3F4 flies. After 36 hours of paraquat exposure, less than 30% of Mmus\PrnpP101L.Scer\UAS.T:Hsap\PRNP-3F4 flies are alive, while more than 60% of control flies survive.

      After 60 hours of exposure to 20mM 3-MA, the number of Mmus\PrnpP101L.Scer\UAS.T:Hsap\PRNP-3F4-expressing (under the control of Scer\GAL4αTub84B.PL) flies alive is significantly reduced compared to control flies. However, 40υM wortmannin treatment does not induce any significant difference in the number of live flies between Scer\GAL4αTub84B.PL/Mmus\PrnpP101L.Scer\UAS.T:Hsap\PRNP-3F4 and control flies.

      Scer\GAL4αTub84B.PL/Mmus\PrnpP101L.Scer\UAS.T:Hsap\PRNP-3F4 flies treated with 40υM LY294002 do not show any difference from control flies.

      Scer\GAL4αTub84B.PL/Mmus\PrnpP101L.Scer\UAS.T:Hsap\PRNP-3F4 flies show a significantly increased susceptibility to LiCl but not rapamycin. Scer\GAL4αTub84B.PL/Mmus\PrnpP101L.Scer\UAS.T:Hsap\PRNP-3F4 flies also show an increased susceptibility to the simultaneous treatment of rapamycin and LiCl that is similar to that of LiCl alone.

      (Park et al., 2011)

      Expression of Mmus\PrnpP101L.Scer\UAS.T:Hsap\PRNP-3F4 driven by Scer\GAL4αTub84B.PL induces severe defects in climbing ability and early death. Type 1 glutamatergic larval neuromusclular junctions expressing Mmus\PrnpP101L.Scer\UAS.T:Hsap\PRNP-3F4 driven by Scer\GAL4αTub84B.PL have more small-sized satellite boutons than those of controls.

      Expression of Mmus\PrnpP101L.Scer\UAS.T:Hsap\PRNP-3F4 driven by Scer\GAL4Act5C.PU induces severe defects in climbing ability and early death.

      When Mmus\PrnpP101L.Scer\UAS.T:Hsap\PRNP-3F4 is expressed via by Scer\GAL4ChAT.7.4, more than 90% of flies fail the climbing test at 20 days of age.

      When Mmus\PrnpP101L.Scer\UAS.T:Hsap\PRNP-3F4 is expressed via by Scer\GAL4elav-C155, more than 90% of flies fail the climbing test at 55 days of age.

      Flies expressing Mmus\PrnpP101L.Scer\UAS.T:Hsap\PRNP-3F4 driven by one of the drivers Scer\GAL4Ddc.PL or Scer\GAL4Tab2-201Y or Scer\GAL4Gli.PS or Scer\GAL4C57 do not lead to any significant climbing defects, as compared to controls.

      (Choi et al., 2010)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      Expression of Mmus\PrnpP101L.Scer\UAS.T:Hsap\PRNP-3F4 significantly enhances the pathogenic eye defects seen upon expression of Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 in the eye (both lines under the control of Scer\GAL4GMR.PF), with approximately 53% of ommatidia displaying aberrant structures.

      Expression of Mmus\PrnpP101L.Scer\UAS.T:Hsap\PRNP-3F4 significantly enhances the pathogenic eye defects seen upon expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 in the eye (both lines under the control of Scer\GAL4GMR.PF), with approximately 92% of ommatidia displaying aberrant structures.

      Expression of Hsap\MJDtr.Q27.Scer\UAS.T:Ivir\HA1 and Mmus\PrnpP101L.Scer\UAS.T:Hsap\PRNP-3F4 in the developing eye under the control of Scer\GAL4GMR.PF does not result in any significant structural defects in the eye.

      Expression of Zzzz\CAG20Q.Scer\UAS.T:Ivir\HA1 and Mmus\PrnpP101L.Scer\UAS.T:Hsap\PRNP-3F4 in the developing eye under the control of Scer\GAL4GMR.PF does not result in any significant structural defects in the eye.

      (Park et al., 2011)

      Co-expression of BacA\p35Scer\UAS.cUa with Mmus\PrnpP101L.Scer\UAS.T:Hsap\PRNP-3F4 driven by Scer\GAL4Cha.7.4 does not suppress the Mmus\PrnpP101L.Scer\UAS.T:Hsap\PRNP-3F4-associated climbing defects.

      (Choi et al., 2010)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Mmus\PrnpP101L.Scer\UAS.T:Hsap\PRNP-3F4
      Mmus\PrnpP101L.UAS.Tag:3F4
      Name Synonyms
      Secondary FlyBase IDs
        References (2)