FB2024_03 , released June 25, 2024
Allele: Dmel\Impβ11UAS.EGFP
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General Information
Symbol
Dmel\Impβ11UAS.EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0246525
Feature type
allele
Associated gene
Dmel\Impβ11
Associated Insertion(s)
Carried in Construct
P{UAS-Impβ11-eGFP}
Key Links
Transgenic product class
cDNA
(Higashi-Kovtun et al., 2010)
wild_type
(Higashi-Kovtun et al., 2010)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Higashi-Kovtun et al., 2010)
wild_type
(Higashi-Kovtun et al., 2010)
Mutagen
in vitro construct
(Higashi-Kovtun et al., 2010)
Progenitor genotype
Carried in construct
P{UAS-Impβ11-eGFP}
Cytology
Description

UASt regulatory sequences drive expression of full length Impβ11 coding sequences (based on the LD41918 EST) tagged with EGFP.

(Higashi-Kovtun et al., 2010)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Impβ11
Tagged with
EGFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4ey.PH/Impβ11UAS.EGFP rescues Impβ1170

      (Higashi-Kovtun et al., 2010)

      Scer\GAL4elav.PLu/Impβ11UAS.EGFP rescues Impβ1170

      (Higashi-Kovtun et al., 2010)

      Scer\GAL4da.G32/Impβ11UAS.EGFP rescues Impβ1170

      (Higashi-Kovtun et al., 2010)

      Scer\GAL4ey.PH/Impβ11UAS.EGFP rescues Impβ119

      (Higashi-Kovtun et al., 2010)

      Scer\GAL4ey.PH/Impβ11UAS.EGFP rescues Impβ1124

      (Higashi-Kovtun et al., 2010)

      Scer\GAL4ey.PH/Impβ11UAS.EGFP rescues Impβ1120

      (Higashi-Kovtun et al., 2010)

      Scer\GAL4ey.PH/Impβ11UAS.EGFP rescues Impβ11202

      (Higashi-Kovtun et al., 2010)
      Fails to rescue

      Scer\GAL4G14/Impβ11UAS.EGFP fails to rescue Impβ1170

      (Higashi-Kovtun et al., 2010)
      Comments

      Expression of Ranbp11Scer\UAS.T:Avic\GFP-EGFP in photoreceptors under the control of Scer\GAL4ey.PH fully restores the on and off transients in Ranbp1170 mutant eye clones.

      Expression of Ranbp11Scer\UAS.T:Avic\GFP-EGFP in photoreceptors under the control of Scer\GAL4ey.PH fully restores the on and off transients in Ranbp119 mutant eye clones.

      Expression of Ranbp11Scer\UAS.T:Avic\GFP-EGFP in photoreceptors under the control of Scer\GAL4ey.PH fully restores the on and off transients in Ranbp1124 mutant eye clones.

      Expression of Ranbp11Scer\UAS.T:Avic\GFP-EGFP in photoreceptors under the control of Scer\GAL4ey.PH fully restores the on and off transients in Ranbp1120 mutant eye clones.

      Expression of Ranbp11Scer\UAS.T:Avic\GFP-EGFP in photoreceptors under the control of Scer\GAL4ey.PH fully restores the on and off transients in Ranbp11202 mutant eye clones.

      Expression of Ranbp11Scer\UAS.T:Avic\GFP-EGFP in all tissues (with a Scer\GAL4da.G32 driver) or in neurons (with a Scer\GAL4elav.PLu driver) rescues the reduction in bouton number/muscle surface area in Ranbp1170 mutants. Expression of Ranbp11Scer\UAS.T:Avic\GFP-EGFP in muscle (with a Scer\GAL4G14 driver) does not rescue this phenotype in Ranbp1170 mutants.

      The observed defects in quantal content and EJP amplitude in Ranbp1170 are fully rescued upon expression of Ranbp11Scer\UAS.T:Avic\GFP-EGFP in the nervous system under the control of Scer\GAL4elav.PLu. Although quantal content is somewhat increased by transgene expression in muscle (under the control of Scer\GAL4G14), it does not correspond to a statistically significant restoration of EJP amplitude. Separately, neither neuronal nor muscle expression of Ranbp11Scer\UAS.T:Avic\GFP-EGFP is adequate to restore mEJP frequency or amplitude to control levels; however, concurrent expression in both neurons and muscle significantly increases mini frequency. mEJP amplitude is not rescued.

      (Higashi-Kovtun et al., 2010)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Impβ11Scer\UAS.T:Avic\GFP-EGFP
      Impβ11UAS.EGFP
      Ranbp11Scer\UAS.T:Avic\GFP-EGFP
      Name Synonyms
      Secondary FlyBase IDs
        References (1)