FlyBase Allele Report: Dmel\HDAC1[12-37]

General Information

Symbol

Dmel\HDAC1^12-37

Species

D. melanogaster

Name

FlyBase ID

FBal0246490

Feature type

allele

Associated gene

Dmel\HDAC1

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Tea et al., 2010)

Progenitor genotype

Cytology

Description

Amino acid replacement: G136R.

(Tea et al., 2010)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:4,627,860..4,627,860 [+]

Nucleotide change:

G4627860M

Amino acid change:

G136R | HDAC1-PA

Reported amino acid change:

G136R

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Tea et al., 2010)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | somatic clone

(Tea et al., 2010)

Phenotype Manifest In

adult antennal lobe projection neuron DL1 adPN | somatic clone

(Tea et al., 2010)

projection neuron | somatic clone

(Tea et al., 2010)

Detailed Description

Statement

Reference

Homozygous neuroblast clones result in a 50% reduction in the number of projection neurons compared to wild type. The mutant cells show a reduced dendritic mass and the dendrites do not target their correct glomeruli.

Homozygous single-cell DL1 projection neuron clones mistarget their dendrites towards dorsomedial (47% of clones) or ventromedial regions (44% of clones) of the antennal lobe (normally the dendrites of this projection neuron project to the dorsolateral glomerulus DL1).

Homozygous DL1 projection neuron clones show an axon overbranching phenotype in the lateral horn, regardless of where the dendrites mistarget.

(Tea et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

HDAC1^12-37 has abnormal neuroanatomy | somatic clone phenotype, suppressible | partially | somatic clone by pros^L.UAS/Scer\GAL4^GH146

(Tea et al., 2010)

NOT suppressed by

Statement

Reference

HDAC1^12-37 has abnormal neuroanatomy | somatic clone phenotype, non-suppressible | somatic clone by Scer\GAL4^GH146/HDAC3^UAS.Tag:FLAG

(Tea et al., 2010)

Phenotype Manifest In

Suppressed by

Statement

Reference

HDAC1^12-37 has adult antennal lobe projection neuron DL1 adPN | somatic clone phenotype, suppressible | partially | somatic clone by pros^L.UAS/Scer\GAL4^GH146

(Tea et al., 2010)

NOT suppressed by

Statement

Reference

HDAC1^12-37 has adult antennal lobe projection neuron DL1 adPN | somatic clone phenotype, non-suppressible | somatic clone by Scer\GAL4^GH146/HDAC3^UAS.Tag:FLAG

(Tea et al., 2010)

HDAC1^12-37 has projection neuron | somatic clone phenotype, non-suppressible | somatic clone by Scer\GAL4^GH146/HDAC3^UAS.Tag:FLAG

(Tea et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

The dendrite targeting and axon arborisation defects seen in Rpd3^12-37 single-cell DL1 projection neuron clones are not rescued by expression in the mutant clone of Hdac3^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^GH146.

The reduction in cell number seen in Rpd3^12-37 projection neuron neuroblast clones is not rescued by expression in the mutant clone of Hdac3^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^GH146.

Expression of pros^L.Scer\UAS under the control of Scer\GAL4^GH146 in DL1 projection neuron clones which are mutant for Rpd3^12-37 rescues the dendrite mistargeting defects seen in these mutant clones. In addition, the axon overbranching phenotype seen in Rpd3^12-37 clones is partially but significantly rescued.

(Tea et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

HDAC1^12-37 is partially rescued by HDAC1^UAS.Tag:V5/Scer\GAL4^GH146

(Tea et al., 2010)

Comments

Expression of Rpd3^{Scer\UAS.T:SV5\V5} under the control of Scer\GAL4^GH146 in single-cell DL1 projection neuron clones which are mutant for Rpd3^12-37 significantly rescues the dendrite mistargeting defects seen in these clones.

Expression of Rpd3^{Scer\UAS.T:SV5\V5} under the control of Scer\GAL4^GH146 in single-cell DL1 projection neuron clones is sufficient to rescue the axon overbranching phenotype in the lateral horn which is seen in these clones.

Expression of Rpd3^{Scer\UAS.T:SV5\V5} under the control of Scer\GAL4^GH146 in projection neuron neuroblast clones which are homozygous for Rpd3^12-37 does not rescue the reduced cell number seen in these clones.

(Tea et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

32238

y¹ w^*; HDAC1^12-37 P{FRT(w^hs)}2A P{neoFRT}82B/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

HDAC1^12-37

(Lenz et al., 2021, Rousseaux et al., 2018)

Rpd3^12-37

(Tea et al., 2010)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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