FB2024_03 , released June 25, 2024
Allele: Dmel\VhlUAS.Tag:HA
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General Information
Symbol
Dmel\VhlUAS.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0246018
Feature type
allele
Associated gene
Dmel\Vhl
Associated Insertion(s)
Carried in Construct
P{UAS-Vhl.HA}
Key Links
Transgenic product class
cDNA
(Duchi et al., 2010)
wild_type
(Duchi et al., 2010)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Duchi et al., 2010)
wild_type
(Duchi et al., 2010)
Mutagen
in vitro construct
(Duchi et al., 2010, Hsouna et al., 2010)
Progenitor genotype
Carried in construct
P{UAS-Vhl.HA}
Cytology
Description

UAS regulatory sequences drive expression of a full-length wild-type Vhl cDNA, which is tagged at the C-terminal end with three copies of Tag:HA.

(Duchi et al., 2010)

UAS regulatory sequences drive expression of full-length Vhl which is tagged at the C-terminal end with three copies of Tag:HA.

(Hsouna et al., 2010)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Vhl
Tagged with
Tag:HA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of VhlScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4btl.PS in a wild-type background does not cause epithelial defects in egg chambers.

      Vhl1 homozygous females which have been rescued to adulthood by expression of VhlScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4btl.PS are sterile, with 70% of the ovarioles containing only degenerated egg chambers. The remaining ovarioles have very severe epithelial defects in their egg chambers, including prominent expansion of the epithelium into the germ cell proper. In other cases, the entire follicle cell epithelium becomes multilayered and the nurse cell complex is collapsed. Microtubules are barely detectable in the mutant egg chambers.

      (Duchi et al., 2010)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      VhlUAS.Tag:HA/Scer\GAL4btl.PS partially rescues Vhl1

      (Duchi et al., 2010)

      VhlUAS.Tag:HA/Scer\GAL4btl.PS partially rescues VhlRNAi.UAS

      (Hsouna et al., 2010)
      Comments

      The lethality of Vhl1 mutants is rescued by expression of VhlScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4btl.PS, although the rescued females are sterile.

      (Duchi et al., 2010)

      The mutant tracheal phenotypes (ectopic migration and abnormal lumen) seen in embryos expressing VhldsRNA.Scer\UAS under the control of Scer\GAL4btl.PS are significantly rescued by co-expression of VhlScer\UAS.T:Ivir\HA1.

      (Hsouna et al., 2010)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      VhlScer\UAS.T:Ivir\HA1
      VhlUAS.Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (2)