female sterile (with Vhl1), with Scer\GAL4btl.PS
egg chamber (with Vhl1), with Scer\GAL4btl.PS
follicle cell (with Vhl1), with Scer\GAL4btl.PS
microtubule (with Vhl1), with Scer\GAL4btl.PS
nurse cell (with Vhl1), with Scer\GAL4btl.PS
Expression of VhlScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4btl.PS in a wild-type background does not cause epithelial defects in egg chambers.
Vhl1 homozygous females which have been rescued to adulthood by expression of VhlScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4btl.PS are sterile, with 70% of the ovarioles containing only degenerated egg chambers. The remaining ovarioles have very severe epithelial defects in their egg chambers, including prominent expansion of the epithelium into the germ cell proper. In other cases, the entire follicle cell epithelium becomes multilayered and the nurse cell complex is collapsed. Microtubules are barely detectable in the mutant egg chambers.
VhlUAS.Tag:HA/Scer\GAL4btl.PS partially rescues Vhl1
VhlUAS.Tag:HA/Scer\GAL4btl.PS partially rescues VhlRNAi.UAS
The lethality of Vhl1 mutants is rescued by expression of VhlScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4btl.PS, although the rescued females are sterile.
The mutant tracheal phenotypes (ectopic migration and abnormal lumen) seen in embryos expressing VhldsRNA.Scer\UAS under the control of Scer\GAL4btl.PS are significantly rescued by co-expression of VhlScer\UAS.T:Ivir\HA1.