FB2024_03 , released June 25, 2024
Allele: Dmel\tllUAS.cKa
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General Information
Symbol
Dmel\tllUAS.cKa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Kurusu
FlyBase ID
FBal0243000
Feature type
allele
Associated gene
Dmel\tll
Associated Insertion(s)
Carried in Construct
P{UAS-tll.K}
Also Known As
UAS-tll
Key Links
Transgenic product class
wild_type
(Kurusu et al., 2009)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Kurusu et al., 2009)
Mutagen
in vitro construct
(Kurusu et al., 2009)
Progenitor genotype
Carried in construct
P{UAS-tll.K}
Cytology
Description

UASt regulatory sequences drive expression of tll.

(Kurusu et al., 2009)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
tll
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  brain cancer
      CEA
      (Hakes and Brand, 2020)
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      model of  brain cancer
      is ameliorated by aseUAS.cBa
      (Hakes and Brand, 2020)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of tllUAS.cKa under the control of Scer\GAL4elav-C155 increases mitotic proliferation of neuroblasts and their daughter cells in the ventral nerve cord during late embryogenesis, compared to controls; expression under the control of Scer\GAL4vg.int2.1 results in the appearance of neuroblasts and their daughter cells in the larval wing disc, which are not seen in controls.

      (Curt et al., 2019)

      Ectopic expression of tllScer\UAS.cKa by Scer\GAL4elav.Switch.PO results in hyperplasia and supernumerary neuroblasts in the 3rd instar larval brain and the mid-pupal brain (40hr after puparium formation).

      (Kurusu et al., 2009)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Suppressed by
      Enhancer of
      Other
      Statement
      Reference

      Doc2UAS.cRa, Scer\GAL4elav-C155, ermUAS.ORF.GW.Tag:HA, otpUAS.ORF.GW.Tag:HA, tllUAS.cKa has increased cell number | embryonic stage phenotype

      (Curt et al., 2019)

      Doc2UAS.cRa, Scer\GAL4pros.PMG, ermUAS.ORF.GW.Tag:HA, otpUAS.ORF.GW.Tag:HA, tllUAS.cKa has increased occurrence of cell division | embryonic stage phenotype

      (Curt et al., 2019)

      Doc2UAS.cRa, Scer\GAL4da.PU, ermUAS.ORF.GW.Tag:HA, otpUAS.ORF.GW.Tag:HA, tllUAS.cKa has abnormal cell number | embryonic stage phenotype

      (Curt et al., 2019)

      Doc2UAS.cRa, Scer\GAL4da.PU, ermUAS.ORF.GW.Tag:HA, otpUAS.ORF.GW.Tag:HA, tllUAS.cKa has increased occurrence of cell division | embryonic stage phenotype

      (Curt et al., 2019)

      Scer\GAL4wg.PU, ermUAS.ORF.GW.Tag:HA, tllUAS.cKa has increased cell number | embryonic stage phenotype

      (Curt et al., 2019)

      Scer\GAL4wg.PU, ermUAS.ORF.GW.Tag:HA, tllUAS.cKa has increased occurrence of cell division | embryonic stage phenotype

      (Curt et al., 2019)

      Scer\GAL4pros.PMG, ermUAS.ORF.GW.Tag:HA, tllUAS.cKa has increased occurrence of cell division | embryonic stage phenotype

      (Curt et al., 2019)

      Scer\GAL4da.PU, ermUAS.ORF.GW.Tag:HA, tllUAS.cKa has increased cell number | embryonic stage phenotype

      (Curt et al., 2019)

      Doc2UAS.cRa, Scer\GAL4wg.PU, ermUAS.ORF.GW.Tag:HA, otpUAS.ORF.GW.Tag:HA, tllUAS.cKa has increased cell number | embryonic stage phenotype

      (Curt et al., 2019)

      Doc2UAS.cRa, Scer\GAL4wg.PU, ermUAS.ORF.GW.Tag:HA, otpUAS.ORF.GW.Tag:HA, tllUAS.cKa has increased occurrence of cell division | embryonic stage phenotype

      (Curt et al., 2019)
      Phenotype Manifest In
      Enhanced by
      Suppressed by
      Enhancer of
      Statement
      Reference

      Doc2UAS.cRa, otpUAS.ORF.GW.Tag:HA, tllUAS.cKa, Scer\GAL4elav-C155 is an enhancer of neuroblast | embryonic stage phenotype of Scer\GAL4elav-C155, ermUAS.ORF.GW.Tag:HA

      (Curt et al., 2019)

      Doc2UAS.cRa, otpUAS.ORF.GW.Tag:HA, tllUAS.cKa, Scer\GAL4elav-C155 is an enhancer of ganglion mother cell | embryonic stage phenotype of Scer\GAL4elav-C155, ermUAS.ORF.GW.Tag:HA

      (Curt et al., 2019)

      otpUAS.ORF.GW.Tag:HA, ermUAS.ORF.GW.Tag:HA, tllUAS.cKa, Scer\GAL4elav-C155 is an enhancer of larval ventral nerve cord | embryonic stage phenotype of Doc2UAS.cRa, Scer\GAL4elav-C155

      (Curt et al., 2019)

      otpUAS.ORF.GW.Tag:HA, ermUAS.ORF.GW.Tag:HA, tllUAS.cKa, Scer\GAL4elav-C155 is an enhancer of neuroblast | embryonic stage phenotype of Doc2UAS.cRa, Scer\GAL4elav-C155

      (Curt et al., 2019)

      otpUAS.ORF.GW.Tag:HA, ermUAS.ORF.GW.Tag:HA, tllUAS.cKa, Scer\GAL4elav-C155 is an enhancer of ganglion mother cell | embryonic stage phenotype of Doc2UAS.cRa, Scer\GAL4elav-C155

      (Curt et al., 2019)

      Doc2UAS.cRa, ermUAS.ORF.GW.Tag:HA, tllUAS.cKa, Scer\GAL4elav-C155 is an enhancer of larval ventral nerve cord | embryonic stage phenotype of Scer\GAL4elav-C155, otpUAS.ORF.GW.Tag:HA

      (Curt et al., 2019)

      Doc2UAS.cRa, ermUAS.ORF.GW.Tag:HA, tllUAS.cKa, Scer\GAL4elav-C155 is an enhancer of neuroblast | embryonic stage phenotype of Scer\GAL4elav-C155, otpUAS.ORF.GW.Tag:HA

      (Curt et al., 2019)

      Doc2UAS.cRa, ermUAS.ORF.GW.Tag:HA, tllUAS.cKa, Scer\GAL4elav-C155 is an enhancer of ganglion mother cell | embryonic stage phenotype of Scer\GAL4elav-C155, otpUAS.ORF.GW.Tag:HA

      (Curt et al., 2019)

      Doc2UAS.cRa, otpUAS.ORF.GW.Tag:HA, tllUAS.cKa, Scer\GAL4elav-C155 is an enhancer of larval ventral nerve cord | embryonic stage phenotype of Scer\GAL4elav-C155, ermUAS.ORF.GW.Tag:HA

      (Curt et al., 2019)
      Other
      Statement
      Reference

      Doc2UAS.cRa, Scer\GAL4elav-C155, ermUAS.ORF.GW.Tag:HA, otpUAS.ORF.GW.Tag:HA, tllUAS.cKa has larval ventral nerve cord | embryonic stage phenotype

      (Curt et al., 2019)

      Doc2UAS.cRa, Scer\GAL4pros.PMG, ermUAS.ORF.GW.Tag:HA, otpUAS.ORF.GW.Tag:HA, tllUAS.cKa has ganglion mother cell | embryonic stage phenotype

      (Curt et al., 2019)

      Doc2UAS.cRa, Scer\GAL4da.PU, ermUAS.ORF.GW.Tag:HA, otpUAS.ORF.GW.Tag:HA, tllUAS.cKa has larval ventral nerve cord | embryonic stage phenotype

      (Curt et al., 2019)

      Doc2UAS.cRa, Scer\GAL4da.PU, ermUAS.ORF.GW.Tag:HA, otpUAS.ORF.GW.Tag:HA, tllUAS.cKa has ganglion mother cell | increased number | embryonic stage phenotype

      (Curt et al., 2019)

      Doc2UAS.cRa, Scer\GAL4da.PU, ermUAS.ORF.GW.Tag:HA, otpUAS.ORF.GW.Tag:HA, tllUAS.cKa has embryonic/larval glial cell | embryonic stage phenotype

      (Curt et al., 2019)

      Doc2UAS.cRa, Scer\GAL4da.PU, ermUAS.ORF.GW.Tag:HA, otpUAS.ORF.GW.Tag:HA, tllUAS.cKa has neuroblast | increased number | embryonic stage phenotype

      (Curt et al., 2019)

      Doc2UAS.cRa, Scer\GAL4da.PU, ermUAS.ORF.GW.Tag:HA, otpUAS.ORF.GW.Tag:HA, tllUAS.cKa has embryonic/larval neuron | increased number | embryonic stage phenotype

      (Curt et al., 2019)

      Scer\GAL4elav-C155, ermUAS.ORF.GW.Tag:HA, tllUAS.cKa has larval ventral nerve cord | embryonic stage phenotype

      (Curt et al., 2019)

      Doc2UAS.cRa, Scer\GAL4elav-C155, ermUAS.ORF.GW.Tag:HA, otpUAS.ORF.GW.Tag:HA, tllUAS.cKa has neuroblast | increased number | embryonic stage phenotype

      (Curt et al., 2019)

      Scer\GAL4elav-C155, ermUAS.ORF.GW.Tag:HA, tllUAS.cKa has neuroblast | increased number | embryonic stage phenotype

      (Curt et al., 2019)

      Scer\GAL4wg.PU, ermUAS.ORF.GW.Tag:HA, tllUAS.cKa has larval ventral nerve cord | embryonic stage phenotype

      (Curt et al., 2019)

      Scer\GAL4wg.PU, ermUAS.ORF.GW.Tag:HA, tllUAS.cKa has neuroblast | embryonic stage phenotype

      (Curt et al., 2019)

      Scer\GAL4wg.PU, ermUAS.ORF.GW.Tag:HA, tllUAS.cKa has ganglion mother cell | embryonic stage phenotype

      (Curt et al., 2019)

      Scer\GAL4pros.PMG, ermUAS.ORF.GW.Tag:HA, tllUAS.cKa has larval ventral nerve cord | embryonic stage phenotype

      (Curt et al., 2019)

      Scer\GAL4pros.PMG, ermUAS.ORF.GW.Tag:HA, tllUAS.cKa has neuroblast | embryonic stage phenotype

      (Curt et al., 2019)

      Scer\GAL4pros.PMG, ermUAS.ORF.GW.Tag:HA, tllUAS.cKa has ganglion mother cell | embryonic stage phenotype

      (Curt et al., 2019)

      Doc2UAS.cRa, Scer\GAL4wg.PU, ermUAS.ORF.GW.Tag:HA, otpUAS.ORF.GW.Tag:HA, tllUAS.cKa has larval ventral nerve cord | embryonic stage phenotype

      (Curt et al., 2019)

      Scer\GAL4da.PU, ermUAS.ORF.GW.Tag:HA, tllUAS.cKa has larval ventral nerve cord | embryonic stage phenotype

      (Curt et al., 2019)

      Scer\GAL4da.PU, ermUAS.ORF.GW.Tag:HA, tllUAS.cKa has ganglion mother cell | increased number | embryonic stage phenotype

      (Curt et al., 2019)

      Scer\GAL4da.PU, ermUAS.ORF.GW.Tag:HA, tllUAS.cKa has neuroblast | increased number | embryonic stage phenotype

      (Curt et al., 2019)

      Scer\GAL4da.PU, ermUAS.ORF.GW.Tag:HA, tllUAS.cKa has embryonic/larval neuron | increased number | embryonic stage phenotype

      (Curt et al., 2019)

      Scer\GAL4da.PU, ermUAS.ORF.GW.Tag:HA, tllUAS.cKa has embryonic/larval glial cell | increased number | embryonic stage phenotype

      (Curt et al., 2019)

      Scer\GAL4vg.int2.1, ermUAS.ORF.GW.Tag:HA, tllUAS.cKa has embryonic/larval neuron | ectopic | larval stage phenotype

      (Curt et al., 2019)

      Scer\GAL4vg.int2.1, ermUAS.ORF.GW.Tag:HA, tllUAS.cKa has embryonic/larval glial cell | ectopic | larval stage phenotype

      (Curt et al., 2019)

      Doc2UAS.cRa, Scer\GAL4wg.PU, ermUAS.ORF.GW.Tag:HA, otpUAS.ORF.GW.Tag:HA, tllUAS.cKa has neuroblast | embryonic stage phenotype

      (Curt et al., 2019)

      Doc2UAS.cRa, Scer\GAL4wg.PU, ermUAS.ORF.GW.Tag:HA, otpUAS.ORF.GW.Tag:HA, tllUAS.cKa has ganglion mother cell | embryonic stage phenotype

      (Curt et al., 2019)

      Doc2UAS.cRa, Scer\GAL4pros.PMG, ermUAS.ORF.GW.Tag:HA, otpUAS.ORF.GW.Tag:HA, tllUAS.cKa has larval ventral nerve cord | embryonic stage phenotype

      (Curt et al., 2019)

      Doc2UAS.cRa, Scer\GAL4pros.PMG, ermUAS.ORF.GW.Tag:HA, otpUAS.ORF.GW.Tag:HA, tllUAS.cKa has neuroblast | embryonic stage phenotype

      (Curt et al., 2019)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of tllUAS.cKa, otpUAS.ORF.GW.Tag:HA, ermUAS.ORF.GW.Tag:HA and Doc2UAS.cRa under the control of Scer\GAL4elav-C155 significantly increases total cell number and the number of neuroblasts in the late embryonic ventral nerve cord, compared to controls; expression under the control of Scer\GAL4wg.PU results in a trend towards increased symmetric (abnormal) neuroblast division and significantly increased asymmetric (normal) daughter (ganglion mother cell) division as well as a significant increase in the number of cells in the NB5-6 lineage, compared to controls; expression under the control of Scer\GAL4pros.PMG significantly increases the number of neuroblasts and daughters in S-phase, with no effect on cell cycle speed, compared to controls; expression under the control of Scer\GAL4da.PU increases the number of mitotic cells, neuroblasts, daughters and neurons, and reduces the number of glia, with a thickening of the CNS, compared to controls.

      Expression of tllUAS.cKa and ermUAS.ORF.GW.Tag:HA under the control of Scer\GAL4elav-C155 significantly increases the number of neuroblasts in the late embryonic ventral nerve cord, compared to controls; expression under the control of Scer\GAL4wg.PU results in a significant increase in symmetric (abnormal) neuroblast division and asymmetric (normal) daughter (ganglion mother cell) division as well as a significant increase in the number of cells in the NB5-6 lineage, compared to controls; expression under the control of Scer\GAL4pros.PMG significantly increases the number of neuroblasts and daughters in S-phase, with no effect on cell cycle speed, compared to controls; expression under the control of Scer\GAL4da.PU increases the number of mitotic cells, neuroblasts, neuroblast daughters, neurons and glia, compared to controls; expression under the control of Scer\GAL4[vg.int2.1] results in the formation of neurons and glia in the larval wing disc, these are not seen in controls.

      (Curt et al., 2019)

      Co-overexpression of prosScer\UAS.cMa and tllScer\UAS.cKa by Scer\GAL4elav.Switch.PO rescues the tllScer\UAS.cKa-induced brain hyperplasia in third instar larvae.

      (Kurusu et al., 2009)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4elav-C155/tllUAS.cKa rescues tll1

      (Kurusu et al., 2009)
      Comments

      Rescues the tll1 mutant phenotype of 10.4 neurons per mushroom body clone to 108.

      (Kurusu et al., 2009)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      tllScer\UAS.cKa
      tllUAS.cKa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Kurusu
      Secondary FlyBase IDs
        References (6)