FlyBase Allele Report: Dmel\trol[JF03376]

General Information

Symbol

Dmel\trol^JF03376

Species

D. melanogaster

Name

FlyBase ID

FBal0242548

Feature type

allele

Associated gene

Dmel\trol

Associated Insertion(s)

Carried in Construct

P{TRiP.JF03376}

Also Known As

UAS-trol.RNAi^TRiP.JF03376

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Perkins et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Perkins et al., 2009)

Mutagen

in vitro construct

(Perkins et al., 2009)

Progenitor genotype

Carried in construct

P{TRiP.JF03376}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat.

(Perkins et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-JF03376

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult posterior midgut epithelium, with Scer\GAL4^NP6267

(Takemura and Nakato, 2017)

cuboidal/columnar epithelium | larval stage, with Scer\GAL4^C-765, Scer\GAL4^ppl.PP

(Bonche et al., 2022)

embryonic/larval brain | third instar larval stage, with Scer\GAL4^αTub84B.PL

(Skeath et al., 2017)

larval ventral nerve cord | third instar larval stage, with Scer\GAL4^αTub84B.PL

(Skeath et al., 2017)

squamous epithelium | larval stage, with Scer\GAL4^Act5C.PI

(Bonche et al., 2022)

squamous epithelium | larval stage, with Scer\GAL4^C-765

(Bonche et al., 2022)

squamous epithelium | larval stage, with Scer\GAL4^C-765, Scer\GAL4^ppl.PP

(Bonche et al., 2022)

squamous epithelium | larval stage, with Scer\GAL4^ppl.PP

(Bonche et al., 2022)

wing disc | larval stage, with Scer\GAL4^Act5C.PI

(Bonche et al., 2022)

wing disc | larval stage, with Scer\GAL4^C-765

(Bonche et al., 2022)

wing disc | larval stage, with Scer\GAL4^C-765, Scer\GAL4^ppl.PP

(Bonche et al., 2022)

wing disc | larval stage, with Scer\GAL4^ppl.PP

(Bonche et al., 2022)

Detailed Description

Statement

Reference

The expression of trol^JF03376 under the control of Scer\GAL4^Act.PU leads to normal-looking pupae.

(Dai et al., 2018)

Expression of trol^JF03376 under the control of Scer\GAL4^αTub84B.PL results in neural invasion phenotype (neural cells from the brain invade adjacent peripheral structures) as well as protrusions of varied size from the brain and nerve cord.

(Skeath et al., 2017)

Flies expressing trol^JF03376 under the control of Scer\GAL4^NP6267 (using tub-Gal80[ts] to limit the time of expression to adulthood) display reduced regenerative cell proliferation in the adult posterior midgut upon infection with Pseudomonas entomophila.

(Takemura and Nakato, 2017)

trol knockdown through expression of trol^JF03376 under the control of Scer\GAL4^Act5C.PI results in more compact looking imaginal discs, with sections showing elongation of cells in the apico-basal axis. in addition, the central nervous system shows signs of hyper-constriction, with vesicles of tissue herniating from the ventral nerve cord.

(Pastor-Pareja and Xu, 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Ndg^HMJ24142, Scer\GAL4^Act.PU, trol^JF03376 has decreased body size | pupal stage phenotype

(Dai et al., 2018)

Ndg^HMJ24142, Scer\GAL4^Act.PU, trol^JF03376 has visible | pupal stage phenotype

(Dai et al., 2018)

Ndg^HMJ24142, Scer\GAL4^Act.PU, trol^JF03376 has lethal - all die before end of P-stage phenotype

(Dai et al., 2018)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The co-expression of Ndg^HMJ24142 and trol^JF03376 under the control of Scer\GAL4^Act.PU leads to small pupae that do not develop to adulthood, as compared to either single knockdown and wild-type controls; this phenotype is more severe at 30[o]C than at 25[o]C.

(Dai et al., 2018)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

29440

y¹ v¹; P{TRiP.JF03376}attP2/TM3, Sb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

trol^JF03376

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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