FlyBase Allele Report: Dmel\ct[JF03304]

General Information

Symbol

Dmel\ct^JF03304

Species

D. melanogaster

Name

FlyBase ID

FBal0242480

Feature type

allele

Associated gene

Dmel\ct

Associated Insertion(s)

Carried in Construct

P{TRiP.JF03304}

Also Known As

UAS-ct-RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Perkins et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Perkins et al., 2009)

Mutagen

in vitro construct

(Perkins et al., 2009)

Progenitor genotype

Carried in construct

P{TRiP.JF03304}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat.

(Perkins et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-JF03304

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^ppk.1.9

(Iyer et al., 2013)

abnormal neuroanatomy | larval stage, with Scer\GAL4⁴⁷⁷

(Bhattacharjee et al., 2023)

visible, with Scer\GAL4^GMR.PU

(Javeed et al., 2015)

Phenotype Manifest In

Detailed Description

Statement

Reference

No obvious wing defects are observed when ct^JF03304 is expressed under the control of Scer\GAL4^Bx-MS1096.

Expression of ct^JF03304 under the control of Scer\GAL4^GMR.PU results in a glossy/sparkling eye phenotype.

(Javeed et al., 2015)

Expression of ct^JF03304 under the control of Scer\GAL4^ppk.1.9 results in strong and penetrant phenotypes in C-IV neurons but no phenotypes in C-I neurons. DdaC, v'ada and vdaB neurons all have reduced total dendritic length compared to controls. The total number of dendritic branches is also reduced in ddaC and v'ada and vdaB neurons.

(Iyer et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Scer\GAL4^GMR.PU, ct^JF03304 has visible phenotype, non-enhanceable by Poxn^BRB, Scer\GAL4^GMR.PU

(Javeed et al., 2015)

Suppressed by

Statement

Reference

Scer\GAL4⁴⁷⁷, ct^JF03304 has abnormal neuroanatomy | larval stage phenotype, suppressible | partially by bdwf^{UAS.Tag:FLAG,Tag:HA}/Scer\GAL4⁴⁷⁷

(Bhattacharjee et al., 2023)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PU, ct^JF03304 has visible phenotype, non-suppressible by Poxn^BRB, Scer\GAL4^GMR.PU

(Javeed et al., 2015)

NOT Enhancer of

Statement

Reference

ct^JF03304, Scer\GAL4^GMR.PU is a non-enhancer of visible phenotype of Poxn^BRB, Scer\GAL4^GMR.PU, ct^BRO

(Javeed et al., 2015)

ct^JF03304, Scer\GAL4^GMR.PU is a non-enhancer of abnormal eye color phenotype of Poxn^BRB, Scer\GAL4^GMR.PU, ct^BRO

(Javeed et al., 2015)

NOT Suppressor of

Statement

Reference

ct^JF03304, Scer\GAL4^GMR.PU is a non-suppressor of visible phenotype of Poxn^BRB, Scer\GAL4^GMR.PU, ct^BRO

(Javeed et al., 2015)

ct^JF03304, Scer\GAL4^GMR.PU is a non-suppressor of abnormal eye color phenotype of Poxn^BRB, Scer\GAL4^GMR.PU, ct^BRO

(Javeed et al., 2015)

ct^JF03304, Scer\GAL4^Bx-MS1096 is a non-suppressor of visible phenotype of Poxn^BRB, Scer\GAL4^Bx-MS1096

(Javeed et al., 2015)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Scer\GAL4^GMR.PU, ct^JF03304 has eye phenotype, non-enhanceable by Poxn^BRB, Scer\GAL4^GMR.PU

(Javeed et al., 2015)

Suppressed by

Statement

Reference

Scer\GAL4⁴⁷⁷, ct^JF03304 has dendrite | larval stage phenotype, suppressible | partially by bdwf^{UAS.Tag:FLAG,Tag:HA}/Scer\GAL4⁴⁷⁷

(Bhattacharjee et al., 2023)

Scer\GAL4⁴⁷⁷, ct^JF03304 has larval multidendritic class IV neuron phenotype, suppressible | partially by bdwf^{UAS.Tag:FLAG,Tag:HA}/Scer\GAL4⁴⁷⁷

(Bhattacharjee et al., 2023)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PU, ct^JF03304 has eye phenotype, non-suppressible by Poxn^BRB, Scer\GAL4^GMR.PU

(Javeed et al., 2015)

NOT Enhancer of

Statement

Reference

ct^JF03304, Scer\GAL4^GMR.PU is a non-enhancer of eye phenotype of Poxn^BRB, Scer\GAL4^GMR.PU, ct^BRO

(Javeed et al., 2015)

NOT Suppressor of

Statement

Reference

ct^JF03304, Scer\GAL4^GMR.PU is a non-suppressor of eye phenotype of Poxn^BRB, Scer\GAL4^GMR.PU, ct^BRO

(Javeed et al., 2015)

ct^JF03304, Scer\GAL4^Bx-MS1096 is a non-suppressor of wing vein | ectopic phenotype of Poxn^BRB, Scer\GAL4^Bx-MS1096

(Javeed et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of ct^JF03304 does not suppress the wing phenotypes seen when Poxn^BRB is expressed under the control of Scer\GAL4^Bx-MS1096.

Co-expression of Poxn^BRB and ct^BRO under the control of Scer\GAL4^GMR.PU produces an eye phenotype that is qualitatively very similar to when either transgene is expressed alone. This phenotype is not suppressed upon co-expression of ct^JF03304.

(Javeed et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

ct^JF03304 is rescued by ct^BRO/Scer\GAL4^GMR.PU

(Javeed et al., 2015)

Partially rescued by

ct^JF03304 is partially rescued by ct^UAS.cPa/Scer\GAL4²²¹

(Bhattacharjee et al., 2023)

Partially rescues

ct^JF03304/Scer\GAL4^GMR.PU partially rescues ct^BRO

(Javeed et al., 2015)

ct^JF03304/Scer\GAL4^Bx-MS1096 partially rescues ct^BRO

(Javeed et al., 2015)

ct^JF03304/Scer\GAL4^Bx-MS1096 partially rescues ct^UAS.cPa

(Javeed et al., 2015)

Comments

Expression of ct^JF03304 partially rescues the wing phenotypes seen when ct^Scer\UAS.cPa is expressed under the control of Scer\GAL4^Bx-MS1096, although the posterior region remains mispatterned.

Expression of ct^JF03304 substantially rescues the wing phenotypes seen when ct^BRO is expressed under the control of Scer\GAL4^Bx-MS1096. Only minor defects remain, such as a weak posterior margin.

Co-expression of ct^BRO and ct^JF03304 under the control of Scer\GAL4^GMR.PU suppresses the eye phenotypes seen when either transgene is expressed alone. Only a slight reduction in pigmentation remains.

(Javeed et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

29625

y¹ v¹; P{TRiP.JF03304}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

ct^JF03304

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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