FB2024_03 , released June 25, 2024
Allele: Dmel\Gr32aKO
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General Information
Symbol
Dmel\Gr32aKO
Species
D. melanogaster
Name
FlyBase ID
FBal0241775
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
ΔGr32a, Gr32a-, Gr32a1
Key Links
Nature of the Allele
Associated Insertion(s)
Cytology
Description

Precise replacement of the Gr32a gene by a mini-w marker.

Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DNA sequence
Protein sequence
 
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Gr32aKO mutant flies do not show significant changes in the expected avoidance response to food laced with acetic acid (5%), as compared to controls.

Gr32aKO severely reduces the I-a, S-a and S-b sensilla responsiveness to the bitter chemicals LOB, BER, DEN normally observed in controls; there are no changes in I-b sensilla responsiveness to LOB, BER, DEN.

Gr32aKO mutant flies exhibit mild defects on strychnine avoidance compared with controls.

Gr32aKO mutant adults show normal avoidance of umbelliferone in a two-way choice feeding assay.

Mutant males show reduced aggressive behavior (significantly increased latency to the first lunge and significantly reduced number of lunges) compared to controls. The mutant males show significantly increased levels of male-male courtship compared to controls.

Homozygous Gr32aKO mutant larvae do not have altered preference for larval-treated substrate, compared to controls.

Mutant flies show normal L-canavanine avoidance in a two-way choice test.

When paired with control males, Gr32aKO males show a diminished aggression level compared to Gr32aKO/+ control males.

When paired with control males, Gr32aKO males do not show a change in the level of male-male courtship towards control males, although they do show impaired courtship towards oenoctye-eliminated target males perfumed with (z)-7-tricosene).

Gr32aKO males fail to show increased aggression and decreased courtship towards oenocyte-eliminated targets perfumed with (z)-7-tricosene. In contrast, Gr32aKO heterozygotes, as in wild-type males, show increased aggression and decreased courtship towards oenocyte-eliminated males.

The addition of synthetic 11-cis-vaccenyl acetate (cVA) does not promote aggression in Gr32aKO males, although it does so in Gr32aKO heterozygotes and Gr32aKO flies that have been rescued by Gr32a+t5.9.

Mutant flies show no difference from wild-type flies in a proboscis extension response assay in response to either 100mM sucrose, or to 100mM sucrose containing a bitter compound (one of 100mM caffeine, 100mM quinine or 1mM Denatonium).

Homozygous males show an increased courtship index towards decapitated males compared to the courtship index of wild-type males in this assay. Heterozygous males show a courtship index intermediate between that of homozygous and wild-type males in this assay.

Homozygous males show later courtship steps towards males (wing vibration and attempting) much more frequently than do wild-type males.

Homozygous virgin females mate with wild-type males as efficiently as do wild-type virgin females in one-on-one mating assays.

Homozygous males mate with virgin females as efficiently as do wild-type males in single pair courtship assays. However, when two males compete for a single virgin female, a wild-type males outperforms a homozygous male by a ratio of 4 to 1.

Homozygous males show the same courtship activity towards decapitated females as do wild-type males.

Homozygous males show an increased courtship index towards decapitated males compared to the courtship index of wild-type males in this assay.

Homozygous males show an increased courtship index towards mated females compared to the courtship index of wild-type males in this assay.

Homozygous males show an increased courtship index towards male-conditioned females (females that have been exposed to males but are virgins) compared to the courtship index of wild-type males in this assay.

External Data
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Phenotypic Class
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Additional Comments
Genetic Interactions
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Xenogenetic Interactions
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Reference
Complementation and Rescue Data
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Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
References (18)