Gr32aKO mutant flies do not show significant changes in the expected avoidance response to food laced with acetic acid (5%), as compared to controls.
Gr32aKO severely reduces the I-a, S-a and S-b sensilla responsiveness to the bitter chemicals LOB, BER, DEN normally observed in controls; there are no changes in I-b sensilla responsiveness to LOB, BER, DEN.
Gr32aKO mutant flies exhibit mild defects on strychnine avoidance compared with controls.
Gr32aKO mutant adults show normal avoidance of umbelliferone in a two-way choice feeding assay.
Mutant males show reduced aggressive behavior (significantly increased latency to the first lunge and significantly reduced number of lunges) compared to controls. The mutant males show significantly increased levels of male-male courtship compared to controls.
Homozygous Gr32aKO mutant larvae do not have altered preference for larval-treated substrate, compared to controls.
Mutant flies show normal L-canavanine avoidance in a two-way choice test.
When paired with control males, Gr32aKO males show a diminished aggression level compared to Gr32aKO/+ control males.
When paired with control males, Gr32aKO males do not show a change in the level of male-male courtship towards control males, although they do show impaired courtship towards oenoctye-eliminated target males perfumed with (z)-7-tricosene).
Gr32aKO males fail to show increased aggression and decreased courtship towards oenocyte-eliminated targets perfumed with (z)-7-tricosene. In contrast, Gr32aKO heterozygotes, as in wild-type males, show increased aggression and decreased courtship towards oenocyte-eliminated males.
The addition of synthetic 11-cis-vaccenyl acetate (cVA) does not promote aggression in Gr32aKO males, although it does so in Gr32aKO heterozygotes and Gr32aKO flies that have been rescued by Gr32a+t5.9.
Mutant flies show no difference from wild-type flies in a proboscis extension response assay in response to either 100mM sucrose, or to 100mM sucrose containing a bitter compound (one of 100mM caffeine, 100mM quinine or 1mM Denatonium).
Homozygous males show an increased courtship index towards decapitated males compared to the courtship index of wild-type males in this assay. Heterozygous males show a courtship index intermediate between that of homozygous and wild-type males in this assay.
Homozygous males show later courtship steps towards males (wing vibration and attempting) much more frequently than do wild-type males.
Homozygous virgin females mate with wild-type males as efficiently as do wild-type virgin females in one-on-one mating assays.
Homozygous males mate with virgin females as efficiently as do wild-type males in single pair courtship assays. However, when two males compete for a single virgin female, a wild-type males outperforms a homozygous male by a ratio of 4 to 1.
Homozygous males show the same courtship activity towards decapitated females as do wild-type males.
Homozygous males show an increased courtship index towards decapitated males compared to the courtship index of wild-type males in this assay.
Homozygous males show an increased courtship index towards mated females compared to the courtship index of wild-type males in this assay.
Homozygous males show an increased courtship index towards male-conditioned females (females that have been exposed to males but are virgins) compared to the courtship index of wild-type males in this assay.
Gr32aKO is rescued by Gr32a+t5.9
Gr32aKO is rescued by Scer\GAL4Gr32a.3.776/Gr32aUAS.cMa
Gr32aKO is rescued by Scer\GAL4VP16.Gr32a.3780/Gr32aUAS.cMa
Gr32aKO is partially rescued by Gr32a+t5.9