FlyBase Allele Report: Dmel\wrd[RD.UAS]

General Information

Symbol

Dmel\wrd^RD.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0241371

Feature type

allele

Associated gene

Dmel\wrd

Associated Insertion(s)

Carried in Construct

P{UAS-wrd.RD}

Key Links

Transgenic product class

wild_type

(Viquez et al., 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Viquez et al., 2006)

Mutagen

in vitro construct

(Viquez et al., 2006)

Progenitor genotype

Carried in construct

P{UAS-wrd.RD}

Cytology

Description

UASt regulatory sequences drive expression of the RD splice form of wrd.

(Viquez et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

wrd

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage, with Scer\GAL4^elav-C155

(Viquez et al., 2006)

Phenotype Manifest In

neuromuscular junction | larval stage, with Scer\GAL4^elav-C155

(Viquez et al., 2006)

NMJ bouton | larval stage, with Scer\GAL4^elav-C155

(Viquez et al., 2006)

Detailed Description

Statement

Reference

Overexpression of PP2A-B'^RD.Scer\UAS using Scer\GAL4^elav-C155 leads to synaptic overgrowth in all type I neuromuscular junctions, with a 33% increase in bouton number, and a 20% decrease in bouton size relative to wild-type.

Neuronal expression of PP2A-B'^RD.Scer\UAS under the control of Scer\GAL4^elav.PLu in an otherwise wild-type background leads to a significant decrease in quantal content.

(Viquez et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

Scer\GAL4^elav-C155/wrd^RD.UAS partially rescues wrd¹⁰⁴

(Viquez et al., 2006)

Scer\GAL4^G7/wrd^RD.UAS partially rescues wrd¹⁰⁴

(Viquez et al., 2006)

Comments

Neuronal expression of PP2A-B'^RD.Scer\UAS under the control of Scer\GAL4^elav-C155 rescues the morphological phenotype associated with PP2A-B'¹⁰⁴, leading to a 54% decrease in bouton size and a 161% increase in bouton number relative to PP2A-B'¹⁰⁴ homozygotes. However, quantal content is not rescued.

Muscle-specific overexpression of PP2A-B'^RD.Scer\UAS using Scer\GAL4^G7 in the homozygous PP2A-B'¹⁰⁴ mutant background results in a modest reduction in bouton size, although boutons remain 37% larger than in wild-type larvae. Muscle-specific expression of PP2A-B'^RD.Scer\UAS rescues the quantal content defect found in a homozygous PP2A-B'¹⁰⁴ mutant background.

However, the muscle-specific overexpression does rescue the deficient evoked transmitter release of PP2A-B'¹⁰⁴ homozygotes. Neuronal expression of PP2A-B'^RD.Scer\UAS in PP2A-B'¹⁰⁴ homozygotes decreases the proportion of unbundled microtubules seen in the mutants alone.

(Viquez et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

PP2A-B'^RD.Scer\UAS

wrd^RD.Scer\UAS

wrd^RD.UAS

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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