FB2024_03 , released June 25, 2024
Allele: Dmel\hbsRNAi.UAS.cBa
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General Information
Symbol
Dmel\hbsRNAi.UAS.cBa
Species
D. melanogaster
Name
FlyBase ID
FBal0240396
Feature type
allele
Associated gene
Dmel\hbs
Associated Insertion(s)
Carried in Construct
P{UAS-hbs.RNAi.B}
Key Links
Transgenic product class
RNAi_reagent
(Bao and Cagan, 2005)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Bao and Cagan, 2005)
Mutagen
in vitro construct
(Bao and Cagan, 2005)
Progenitor genotype
Carried in construct
P{UAS-hbs.RNAi.B}
Cytology
Description

UASt sequences control expression of an inverted repeated hbs (167-670 bp) for RNAi.

(Bao and Cagan, 2005)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
hbs
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Moderate reduction of hbs through the expression of hbsIR.Scer\UAS under the control of Scer\GAL4GMR.PF results in defects in inter-ommatidial cells and bristle group positioning.

      Strong reduction of hbsIR.Scer\UAS under the control of Scer\GAL4GMR.PF (in the presence of Dcr-2Scer\UAS.cDa to increase RNAi efficiency) leads to defects in the organization of cone cells and primary cells as well as in inter-ommatidial cells. Most inter-ommatidial cells sort into a single file and ommatidial spacing is not significantly affected. Less than 4% of ommatidia are found in direct contact with each other.

      (Bao et al., 2010)

      In the developing eyes of hbsIR.Scer\UAS; Scer\GAL4GMR.PF animals at 24 hours APF, ommatidial cores are largely normal, but inter-ommatidial precursor cells (IPCs) fail to sort into a single line as in wild-type. These IPCs have an abnormally relaxed morphology - failing to maximize their contacts with developing primary pigment cells or to minimize their contacts with other IPCs. In addition, inter-ommatidial bristle precursors are often missing or misplaced. The resulting adults have rough eyes dues to disorganized rows of ommatidia.

      (Bao and Cagan, 2005)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Phenotype Manifest In
      Enhanced by
      NOT Enhanced by
      Enhancer of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Simultaneous disruption of sns and hbs through co-expression of snsdsRNA.Scer\UAS and hbsIR.Scer\UAS leads to the direct contact of 27.2% of ommatidia and complete failure of inter-ommatidial cells to sort into single file. These phenotypes represent a strong enhancement of either knockdown alone.

      Reduction of both hbs and kirre expression through kirredsRNA.Scer\UAS and hbsIR.Scer\UAS expression under the control of Scer\GAL4GMR.PF enhances slightly the patterning defects seen in kirre down-regulation. Depletion occasionally leads to inter-ommatidial cells occurring in multiple rows between ommatidia. In each case, ommatidial spacing is nonetheless nearly indistinguishable from single knock-down of hbs alone.

      (Bao et al., 2010)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      hbsIR.Scer\UAS
      hbsRNAi.UAS.cBa
      hbsdsRNA.Scer\UAS.cBa
      hbsdsRNA.UAS.cBa
      Name Synonyms
      Secondary FlyBase IDs
        References (4)