FlyBase Allele Report: Dmel\TrpA1[1]

General Information

Symbol

Dmel\TrpA1¹

Species

D. melanogaster

Name

FlyBase ID

FBal0240383

Feature type

allele

Associated gene

Dmel\TrpA1

Associated Insertion(s)

TI{TI}TrpA1¹

Carried in Construct

Also Known As

dTrpA1¹, trpA¹

Key Links

Allele class

Mutagen

gene targeting by homologous recombination

Nature of the Allele

Allele class

Mutagen

gene targeting by homologous recombination

(Kwon et al., 2008)

Progenitor genotype

Associated Insertion(s)

TI{TI}TrpA1¹

Cytology

Description

Deletion of the sixth transmembrane domain of TrpA1 (amino acid residues 1004-1039). A w^+mW.hs marker is inserted in place of the deleted sequence.

(Kwon et al., 2008)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult olfactory receptor neuron ab11a

(Kwon et al., 2010)

Detailed Description

Statement

Reference

TrpA1¹ mutant larvae have significantly reduced nociceptive movement responses following stimulation with a von Frey filament, compared to controls.

(Hu et al., 2017)

TrpA1¹ mutant larvae have severely reduced rolling (escape) behavior in response to noxious temperature increases, compared to controls.

(Luo et al., 2017)

TrpA1¹ flies show lack of uracil-dependent defecation.

(Du et al., 2016)

TrpA1¹ mutant adults exhibit preference for higher temperatures in a thermotaxis assay than wild type flies. TrpA1¹ mutant larvae exhibit a significant delay in initiation of escape behavior in response to touch with a 46[o]C probe, as compared to wild type.

(Peng et al., 2016)

TrpA1¹ homozygous mutant third instar larvae show impaired temperature selection and unlike controls display no strong preference for cooler temperatures (18[o]C) in a temperature gradient.

(Sokabe et al., 2016)

TrpA1¹ mutant adults show normal preference for less solid sucrose-agarose food than a harder one (higher agarose conc., same sucrose content) one in a two-way food choice assay.

(Zhang et al., 2016)

Homozygous larvae and adults show an impaired nociceptive heat response. Mutant larvae show a normal response to gentle touch. Mutant adults show normal geotactic climbing ability.

(Jang et al., 2015)

In flies carrying TrpA1¹, more than 40% of the larvae are found n the warm side of a plate with a 30[o] and 22[o]C side, reflecting a random distribution across the plate.

(Janssens et al., 2014)

At a constant temperature of 25[o]C, the evening anticipation shown by mutant flies under 12 hour light/12 hour dark conditions is similar to that seen in wild -type flies. The mutant flies also show morning anticipation, although it appears to be slightly reduced. Free-running flies under constant darkness conditions retain morning and evening peaks and the periodicities are similar to wild type. Morning anticipation under constant darkness also appears to be reduced in the mutant flies under constant darkness.

Mutant flies show differences in activity compared to wild type under thermophase/cryophase (TC) conditions (flies entrained initially under 12 hour light/12 hour dark conditions for 4 days at 18[o]C, followed by 7 days of thermophase (18 hours at 29[o]C) and cryophase (6 hours at 18[o]C) cycles in constant darkness). During the first TC cycle, the mutant flies show double peaks, with the second peak approximately coinciding with the original morning peak established during the light-dark entrainment. The morning peaks during the TC cycles are reduced. The evening activity shown by the mutant flies initiates slightly earlier than wild type and is broader. The mutants require an additional day during the TC cycling compared to wild-type to establish a constant period between the evening activity peaks.

Mutant flies show a shortened periodicity of activity during a 29[o]C free run following 29[o]/18[o] TC cycles and during the 16[o] free run following 25[o]/16[o] TC cycles.

(Lee and Montell, 2013)

The gustatory aversion of mutant flies to 6mM camphor in a two way-choice test is not significantly different from that seen in wild-type flies.

(Zhang et al., 2013)

Mutant larvae show defects in discrimination between 18[o]C and 24[o]C in a binary choice thermotaxis assay compared to controls.

Mutant larvae show reduced turning behaviour compared to control larvae when they move into a 24[o]C zone from an 18[o]C zone (turning behaviour when they move from an 18[o]C zone into a 24[o]C zone is similar in both mutant and wild-type larvae).

(Shen et al., 2011)

TrpA1¹ flies exhibit the wild type preference for 5mM sucrose over 1mM sucrose and display wild type avoidance of most bitter chemicals tested in food choice assays. However, mutants display a reduced aversion to aristolochic acid compared to wild type.

TrpA1¹ flies show a similar frequency of action potentials to wild type in response to sucrose, caffeine and most other bitter compounds in tip recordings of sensillum s6. However, in contrast to wild type, mutants have virtually no aristolochic acid-induced action potentials.

(Kim et al., 2010)

Gustatory responses of wild-type but not TrpA1¹ flies to sucrose are suppressed by allyl isothiocyanate.

(Kohno et al., 2010)

The citronellal-evoked action potential frequency in ab12 basiconic sensilla in the antenna of mutant flies is not significantly different from wild type.

(Kwon et al., 2010)

Mutant flies show greatly reduced avoidance of citronellal in a direct airborne repellent test (DART) assay.

Mutant flies show normal avoidance of benzaldehyde in a direct airborne repellent test (DART) assay.

ab11a olfactory receptor neurons (ORNs) in the antenna show a higher citronellal-evoked action potential frequency in mutant flies compared to in wild type. The ab11a ORNs also show a defect in deactivation in the mutant flies.

(Kwon et al., 2010)

Mutant third instar larvae show the same preference for 17.5[o]C over 14[o]C in a two-way choice test as do wild-type controls.

The normal preference of third instar larvae for 17.5[o]C over either 22[o] or 24[o]C in a two-way choice test is eliminated in mutant larvae.

(Kwon et al., 2010)

TrpA1¹ mutants do not exhibit thermotaxis (i.e. a preference to move across a temperature gradient). The ability of TrpA1¹ mutants to chose 18 over 19, 20 or 22[o]c is also eliminated. However, TrpA1¹ larvae do discern 18[o]C from 14-16[o]c. similarly to wild-type, and they prefer 18[o]C over 26-32[o]C, but to a lesser extent than wild-type.

(Kwon et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

TrpA1¹ has abnormal pain response phenotype, suppressible | partially by Tmer\TrpA1^UAS.b/Scer\GAL4^ppk.1.9

(Peng et al., 2016)

TrpA1¹ has abnormal thermotaxis phenotype, suppressible | partially by Vdes\TrpA1^UAS.L/Scer\GAL4^TrpA1.PU

(Peng et al., 2016)

TrpA1¹ has abnormal thermotaxis phenotype, suppressible | partially by Scer\GAL4^TrpA1.PU/Tmer\TrpA1^UAS.b

(Peng et al., 2016)

TrpA1¹ has abnormal pain response phenotype, suppressible | partially by Vdes\TrpA1^UAS.S/Scer\GAL4^ppk.1.9

(Peng et al., 2016)

TrpA1¹ has abnormal pain response phenotype, suppressible by Vdes\TrpA1^UAS.L/Scer\GAL4^ppk.1.9

(Peng et al., 2016)

TrpA1¹ has abnormal taste perception | recessive phenotype, suppressible | partially by Scer\GAL4^TrpA1.PR/Amel\HsTRPA^UAS.cKa

(Kohno et al., 2010)

TrpA1¹ has abnormal chemosensory behavior | adult stage phenotype, suppressible by Agam\trpA1^UAS.A.cKa/Scer\GAL4^TrpA1.PR

(Kwon et al., 2010)

TrpA1¹ has abnormal chemosensory behavior | adult stage phenotype, suppressible by Agam\trpA1^UAS.B.cKa/Scer\GAL4^TrpA1.PR

(Kwon et al., 2010)

NOT suppressed by

Statement

Reference

TrpA1¹ has abnormal thermotaxis phenotype, non-suppressible by Scer\GAL4^TrpA1.PU/Vdes\TrpA1^UAS.S

(Peng et al., 2016)

Suppressor of

Statement

Reference

TrpA1¹ is a suppressor | partially of abnormal behavior | larval stage phenotype of Fa2h^KO1

(Li et al., 2024)

TrpA1¹/Scer\GAL4^TrpA1.PR is a suppressor of abnormal thermotaxis phenotype of Scer\GAL4^TrpA1.PR, norpA^UAS.ckb

(Kwon et al., 2008)

NOT Suppressor of

Statement

Reference

TrpA1¹ is a non-suppressor of abnormal thermotaxis phenotype of Gαq¹

(Kwon et al., 2008)

Other

Statement

Reference

TrpA1¹, pain^EP2451/pain[+] has abnormal pain response | dominant | larval stage phenotype

(Jang et al., 2015)

TrpA1¹, pain^EP2451/pain[+] has abnormal pain response | dominant | adult stage phenotype

(Jang et al., 2015)

TrpA1[+]/TrpA1¹, subdued^Δ5265 has abnormal pain response | dominant | larval stage phenotype

(Jang et al., 2015)

TrpA1[+]/TrpA1¹, subdued^Δ5265 has abnormal pain response | dominant | adult stage phenotype

(Jang et al., 2015)

Scer\GAL4^cry.PU, TrpA1¹, hid^UAS.cUa has abnormal locomotor rhythm phenotype

(Lee and Montell, 2013)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

TrpA1¹ subdued^Δ5265 double heterozygous larvae and adults show an impaired nociceptive heat response.

pain^EP2451; TrpA1¹ double heterozygous larvae and adults show an impaired nociceptive heat response.

(Jang et al., 2015)

Activity peaks during thermophase/cryophase cycles are virtually eliminated in TrpA1¹ flies expressing W^Scer\UAS.cUa under the control of Scer\GAL4^cry.PU. When these flies are subsequently allowed to free run at a constant temperature (either 18[o]C or 29[o]C) in constant darkness, only 6% show rhythmic locomotor activity.

(Lee and Montell, 2013)

Introduction of TrpA1¹ into a Gα49B¹ background results in loss of temperature selection identical to that resulting from the Gα49B¹ alone.

The thermotactic preference for 18[o]C in norpA^Scer\UAS.ckb; Scer\GAL4^TrpA1.PR larvae is suppressed in a TrpA1¹ heterozygous background.

(Kwon et al., 2008)

Xenogenetic Interactions

Statement

Reference

Expression of Vdes\TrpA1^Scer\UAS.L or Tmer\TrpA1^Scer\UAS.b, but not Vdes\TrpA1^Scer\UAS.S, under the control of Scer\GAL4^TrpA1.PU partially suppresses the increased temperature preference seen in TrpA1¹ mutant flies, but also leads to a broader temperature range preference as compared to wild type.

Expression of Vdes\TrpA1^Scer\UAS.L under the control of Scer\GAL4^TrpA1.PU fully suppresses, and of Tmer\TrpA1^Scer\UAS.b or Vdes\TrpA1^Scer\UAS.S under the control of Scer\GAL4^ppk.1.9 partially suppresses the delayed initiation of escape behavior in response to touch with a 46[o]C probe seen in TrpA1¹ mutant larvae.

(Peng et al., 2016)

Expression of Amel\HsTRPA^Scer\UAS.cKa under the control of Scer\GAL4^TrpA1.PR restores the avoidance response of TrpA1¹ mutant flies to allyl isothiocyanate.

Expression of Amel\HsTRPA^Scer\UAS.cKa under the control of Scer\GAL4^TrpA1.PR fails to restore the aversive response of TrpA1¹ mutant flies to aristolochic acid.

(Kohno et al., 2010)

Expression of TrpA1^{Scer\UAS.(B).cKa} under the control of either Scer\GAL4^TrpA1.PR or Scer\GAL4^{Orco.2.642.T:Hsim\VP22} rescues the reduced avoidance to citronellal which is seen in TrpA1¹ flies in a direct airborne repellent test (DART) assay.

Expression of TrpA1^{Scer\UAS.B.Kwon} under the control of Scer\GAL4^TrpA1.PR rescues the reduced avoidance to citronellal which is seen in TrpA1¹ flies in a DART assay.

(Kwon et al., 2010)

Complementation and Rescue Data

Rescued by

TrpA1¹ is rescued by TrpA1^UAS.cUa

(Peng et al., 2016)

TrpA1¹ is rescued by Scer\GAL4^tim.PE/TrpA1^UAS.(B).cKa

(Lee and Montell, 2013)

TrpA1¹ is rescued by Scer\GAL4^TrpA1-AB-G4/TrpA1^UAS.(B).cKa

(Lee and Montell, 2013)

TrpA1¹ is rescued by Scer\GAL4^tim.PE/TrpA1^UAS.B.Kwon

(Lee and Montell, 2013)

TrpA1¹ is rescued by TrpA1^UAS.(B).cKa/Scer\GAL4^Gr66a.PD

(Kim et al., 2010)

TrpA1¹ is rescued by TrpA1^UAS.cUa/Scer\GAL4^TrpA1.PR

(Kohno et al., 2010)

TrpA1¹ is rescued by TrpA1^UAS.B.Kwon/Scer\GAL4^unspecified

(Kwon et al., 2010)

TrpA1¹ is rescued by TrpA1^UAS.(B).cKa/Scer\GAL4^{Orco.2.642.Hsim\VP22}

(Kwon et al., 2010)

TrpA1¹ is rescued by TrpA1^UAS.B.Kwon/Scer\GAL4^TrpA1.PR

(Kwon et al., 2010)

TrpA1¹ is rescued by TrpA1^UAS.(B).cKa/Scer\GAL4^TrpA1.PR

(Kwon et al., 2010)

TrpA1¹ is rescued by TrpA1^UAS.(B).cKa/Scer\GAL4^TrpA1.PR

(Kwon et al., 2008)

Partially rescued by

TrpA1¹ is partially rescued by TrpA1^+t21

(Luo et al., 2017)

TrpA1¹ is partially rescued by TrpA1^UAS.(B).cKa/Scer\GAL4^ppk.1.9

(Luo et al., 2017)

Not rescued by

TrpA1^-AB-G4/TrpA1¹ is not rescued by TrpA1^UAS.(B).cKa/Scer\GAL4^TrpA1-AB-G4

(Luo et al., 2017)

TrpA1¹ is not rescued by TrpA1^UAS.(B).cKa/Scer\GAL4^cry.PU

(Lee and Montell, 2013)

Comments

Expression of TrpA1^Scer\UAS.cUa under the control of Scer\GAL4^TrpA1.PU rescues the increased temperature preference seen in TrpA1¹ mutant flies.

Expression of TrpA1^Scer\UAS.cUa under the control of Scer\GAL4^ppk.1.9 rescues the delayed initiation of escape behavior in response to touch with a 46[o]C probe seen in TrpA1¹ mutant larvae.

(Peng et al., 2016)

Expression of TrpA1^{Scer\UAS.(B).cKa} using Scer\GAL4^Gr66a.PD rescues the loss of avoidance to aristolochic acid shown by TrpA1¹ mutants.

Expression of TrpA1^{Scer\UAS.(B).cKa} using Scer\GAL4^Gr66a.PD rescues the loss of aristolochic acid-induced action potentials shown by TrpA1¹ flies in tip recordings of sensillum s6.

(Kim et al., 2010)

Expression of TrpA1^Scer\UAS.cUa under the control of Scer\GAL4^TrpA1.PR restores the aversive response of TrpA1¹ mutant flies to allyl isothiocyanate.

(Kohno et al., 2010)

The TrpA1¹ thermotactic phenotype is rescued by expression of TrpA1^{Scer\UAS.(B).cKa} under the control of Scer\GAL4^TrpA1.PR.

(Kwon et al., 2008)

Images (0)

Mutant

Wild-type

Stocks (10)

Bloomington

36342

TI{TI}TrpA1¹

26504

w¹¹¹⁸; TI{TI}TrpA1¹