FB2024_03 , released June 25, 2024
Allele: Dmel\mib2UAS.cCRa
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General Information
Symbol
Dmel\mib2UAS.cCRa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Carrasco-Rando
FlyBase ID
FBal0239048
Feature type
allele
Associated gene
Dmel\mib2
Associated Insertion(s)
Carried in Construct
P{UAS-mib2.CR}
Key Links
Transgenic product class
cDNA
(Carrasco-Rando and Ruiz-Gómez, 2008)
wild_type
(Carrasco-Rando and Ruiz-Gómez, 2008)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Carrasco-Rando and Ruiz-Gómez, 2008)
wild_type
(Carrasco-Rando and Ruiz-Gómez, 2008)
Mutagen
in vitro construct
(Carrasco-Rando and Ruiz-Gómez, 2008)
Progenitor genotype
Carried in construct
P{UAS-mib2.CR}
Cytology
Description

UASt regulatory sequences drive expression of a synthetic mib2 cDNA.

(Carrasco-Rando and Ruiz-Gómez, 2008)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
mib2
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of two copies of mib2Scer\UAS.cCRa under the control of Scer\GAL4Mef2.PR results in a severe defect in myoblast fusion in embryos, resulting in the formation of mono- or bi-nucleated thin myotubes. These mini-myotubes elongate properly and are able to contract. These embryos show early massive death of the fusion-competent myoblasts.

      Expression of two copies of mib2Scer\UAS.cCRa under the control of Scer\GAL4sns.PS results in a severe defect in myoblast fusion in embryos, resulting in the formation of mono- or bi-nucleated thin myotubes.

      (Carrasco-Rando and Ruiz-Gómez, 2008)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4how-24B/mib2UAS.cCRa rescues mib21

      (Carrasco-Rando and Ruiz-Gómez, 2008)

      mib2UAS.cCRa/Scer\GAL4kirre.PR rescues mib21

      (Carrasco-Rando and Ruiz-Gómez, 2008)
      Comments

      The somatic and visceral mesoderm defects of mib21 embryos are rescued by expression of mib2Scer\UAS.cCRa under the control of either Scer\GAL4how-24B or Scer\GAL4kirre.PR.

      (Carrasco-Rando and Ruiz-Gómez, 2008)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      mib2Scer\UAS.cCRa
      mib2UAS.cCRa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Carrasco-Rando
      Secondary FlyBase IDs
        References (1)