FB2024_03 , released June 25, 2024
Allele: Dmel\SLC22AKK112539
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General Information
Symbol
Dmel\SLC22AKK112539
Species
D. melanogaster
Name
FlyBase ID
FBal0238224
Feature type
allele
Associated gene
Dmel\SLC22A
Associated Insertion(s)
Carried in Construct
P{KK112539}
Key Links
Genomic Maps

Transgenic product class
RNAi_reagent
(Keleman et al., 2009.8.5)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Keleman et al., 2009.8.5)
Mutagen
in vitro construct
(Keleman et al., 2009.8.5)
Progenitor genotype
Carried in construct
P{KK112539}
Cytology
Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
dsRNA-KK112539
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expressing SLC22AKK112539 under the control of Scer\GAL4fkh.PH does not significantly affect the clearing of salivary gland tissue in pupae, as compared to controls.

      (Velentzas et al., 2018)

      Expression of SLC22AKK112539 (with UAS-Dicer2 to enhance RNAi efficiency) either pan-neuronally (Scer\GAL4nSyb.PU), in all mushroom body (MB) neurons (using one of Scer\GAL4238Y, Scer\GAL4ey-OK107, Scer\GAL4GMR13F02 or Scer\GAL4c747) or in dopamine neurons (using Scer\GAL4ple.PU) results in significant enhancement of 3hr memory in an olfactory classical conditioning assay in adult flies. A significant enhancement of memory is also seen when expression is driven by either Scer\GAL4c305a (α'/β' MB neurons) or Scer\GAL4GMR28H05 (α/β MB neurons), but not when driven by Scer\GAL41471 (γ MB neurons).

      Expression of SLC22AKK112539 (with UAS-Dicer2 to enhance RNAi efficiency) in the glia (using one of Scer\GAL4repo.PU or Scer\GAL4nrv2.PU) has no significant effect on 3hr memory in an olfactory classical conditioning assay in adult flies.

      Expression of SLC22AKK112539 (with UAS-Dicer2 to enhance RNAi efficiency) has no significant effect on 3 hour memory in an olfactory classical conditioning assay in adult flies when expression is driven by any one of Scer\GAL4VT064246, Scer\GAL4GH146, Scer\GAL4Mz604 or Scer\GAL4NP2492.

      Odor (Benzaldehyde and Octanol) and shock (electrical) avoidance was normal (tested with Scer\GAL4238Y, Scer\GAL4GMR13F02, Scer\GAL4c747, Scer\GAL4ey-OK107, Scer\GAL4c305a, Scer\GAL4GMR28H05, Scer\GAL4nSyb.PU, Scer\GAL4ple.PU).

      A conditional knock-down of SLC22A after eclosion in all MB neurons using the Scer\GAL4238Y driver (with UAS-Dicer2 to enhance RNAi efficiency and GAL80ts to limit the stage of expression) results in enhanced 3hr memory in an olfactory classical conditioning assay in adult flies whereas knock-down during development (before eclosion) has no effect on memory test performance in adults.

      Expression of SLC22AKK112539 RNAi in all MB neurons (using one of the following drivers Scer\GAL4238Y, Scer\GAL4GMR13F02 with UAS-Dicer2 to enhance RNAi efficiency) results in significant memory acquisition impairment in an olfactory classical conditioning assay in adult flies. Memory acquisition impairment is also seen when expression is driven in the α' and β' MB neurons using the Scer\GAL4c305a driver (with UAS-Dicer2 to enhance RNAi efficiency).

      Expression of SLC22AKK112539 RNAi in all MB neurons using the Scer\GAL4238Y driver (with UAS-Dicer2 to enhance RNAi efficiency) results in significant enhancement of memory retention in in an olfactory classical conditioning assay in adult flies.

      Adult SLC22A knock-down flies (expressing SLC22AKK112539 RNAi in all MB neurons using the Scer\GAL4238Y driver with UAS-Dicer2 to enhance RNAi efficiency) showed enhanced memory retention in an olfactory classical conditioning assay after cold-shock compared to controls.

      Expression of SLC22AKK112539 RNAi in all MB neurons using the Scer\GAL4238Y driver (with UAS-Dicer2 to enhance RNAi efficiency) does not change the rate of memory decay in an olfactory classical conditioning assay in adult flies.

      Adult SLC22A knock-down flies (expressing SLC22AKK112539 RNAi in all MB neurons using the Scer\GAL4238Y driver with UAS-Dicer2 to enhance RNAi efficiency) showed greater interference in a proactive interference memory test in an olfactory classical conditioning assay compared to controls.

      Adult SLC22A knock-down flies (expressing SLC22AKK112539 RNAi in all MB neurons using the Scer\GAL4238Y driver with UAS-Dicer2 to enhance RNAi efficiency) showed lesser interference in a retroactive interference memory test in an olfactory classical conditioning assay compared to controls.

      Supplementing food with dopamine or histidine impairs 6hr memory in a in an olfactory classical conditioning assay in control adult flies and memory-enhanced flies expressing SLC22AKK112539 RNAi under the Scer\GAL4238Y driver (with UAS-Dicer2 to enhance RNAi efficiency) to the same degree.

      Supplementing food with choline improves 6hr memory in an olfactory classical conditioning assay in control adult flies but has no further effect on the memory-enhanced flies expressing SLC22AKK112539 RNAi under the Scer\GAL4238Y driver (with UAS-Dicer2 to enhance RNAi efficiency).

      (Gai et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Double mutant flies expressing both SLC22AKK112539 RNAi and Rac1V12.Scer\UAS.cUa under the Scer\GAL4c747 driver (with UAS-Dicer2 to enhance RNAi efficiency) show phenotype intermediate to either of the single mutants suggesting that the genetic interaction between SLC22A and Rac1 is not epistatic.

      (Gai et al., 2016)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      SLC22AKK112539 is rescued by Scer\GAL4238Y/SLC22AUAS.cGa

      (Gai et al., 2016)

      SLC22AKK112539 is rescued by Scer\GAL4GMR13F02/SLC22AUAS.cGa

      (Gai et al., 2016)
      Comments

      Co-expression of SLC22AScer\UAS.cGa with SLC22AKK112539 RNAi using either Scer\GAL4238Y or Scer\GAL4GMR13F02 driver fully rescues the 3hr enhanced memory phenotype in adult flies in an olfactory classical conditioning assay produced by the SLC22 knock-down.

      (Gai et al., 2016)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      CG7442KK112539
      SLC22AKK112539
      Name Synonyms
      Secondary FlyBase IDs
        References (3)