FlyBase Allele Report: Dmel\sd[KK108877]

General Information

Symbol

Dmel\sd^KK108877

Species

D. melanogaster

Name

FlyBase ID

FBal0235890

Feature type

allele

Associated gene

Dmel\sd

Associated Insertion(s)

Carried in Construct

P{KK108877}

Also Known As

UAS-Sd RNAi, sd RNAi, UAS-sd^RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK108877}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK108877

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size, with Scer\GAL4^c739

(Rohith and Shyamala, 2017)

abnormal size, with Scer\GAL4^ey-OK107

(Rohith and Shyamala, 2017)

abnormal size, with Scer\GAL4^sd-SG29.1

(Rohith and Shyamala, 2017)

atrophy | pupal stage, with Scer\GAL4^Mef2.PR

(Kaya-Çopur et al., 2021)

decreased cell number | third instar larval stage, with Scer\GAL4^repo.PU

(Reddy and Irvine, 2011)

increased cell number | somatic clone, with Scer\GAL4^insc-Mz1407

(Rohith and Shyamala, 2017)

increased cell size, with Scer\GAL4^sd-SG29.1

(Rohith and Shyamala, 2017)

increased cell size | somatic clone, with Scer\GAL4^insc-Mz1407

(Rohith and Shyamala, 2017)

increased occurrence of cell division | cell non-autonomous, with Scer\GAL4^sd-SG29.1

(Rohith and Shyamala, 2017)

lethal - all die during pupal stage, with Scer\GAL4^en-e16E

(Djiane et al., 2013)

visible, with Scer\GAL4^Bx-MS1096

(Schertel et al., 2015)

Phenotype Manifest In

egg chamber, with Scer\GAL4^NP1624

(Berns et al., 2014)

epithelium, with Scer\GAL4^NP1624

(Berns et al., 2014)

eye disc | third instar larval stage, with Scer\GAL4^repo.PU

(Reddy and Irvine, 2011)

ganglion mother cell | cell non-autonomous, with Scer\GAL4^sd-SG29.1

(Rohith and Shyamala, 2017)

ganglion mother cell | somatic clone, with Scer\GAL4^insc-Mz1407

(Rohith and Shyamala, 2017)

indirect flight muscle cell | pupal stage, with Scer\GAL4^Mef2.PR

(Kaya-Çopur et al., 2021)

Kenyon cell, with Scer\GAL4^c739

(Rohith and Shyamala, 2017)

Kenyon cell, with Scer\GAL4^ey-OK107

(Rohith and Shyamala, 2017)

Kenyon cell, with Scer\GAL4^sd-SG29.1

(Rohith and Shyamala, 2017)

mushroom body, with Scer\GAL4^c739

(Rohith and Shyamala, 2017)

mushroom body, with Scer\GAL4^ey-OK107

(Rohith and Shyamala, 2017)

mushroom body, with Scer\GAL4^sd-SG29.1

(Rohith and Shyamala, 2017)

mushroom body | somatic clone, with Scer\GAL4^insc-Mz1407

(Rohith and Shyamala, 2017)

mushroom body neuroblast | cell non-autonomous, with Scer\GAL4^sd-SG29.1

(Rohith and Shyamala, 2017)

wing, with Scer\GAL4^Bx-MS1096

(Schertel et al., 2015)

Detailed Description

Statement

Reference

The expression of sd^KK108877 under the control of Scer\GAL4^Mef2.PR leads to complete indirect flight muscle atrophy at 90h APF.

(Kaya-Çopur et al., 2021)

The expression of sd^KK108877 under the control of Scer\GAL4^ey-OK107, Scer\GAL4^c739 or Scer\GAL4^sd-SG29.1 (always in combination with Dicer-2 expression, for efficient RNAi) leads to significant increases in the mushroom body and the Kenyon cell cluster sizes, as compared to controls; the Scer\GAL4^sd-SG29.1-driven expression also leads to a significant increase in the proportion of mitotic neuroblasts and ganglion mother cells, despite of no change in neuroblast number, in Kenyon cell clusters and leads to a significant increase in Kenyon cell size, as compared to controls. Clonal expression of sd^KK108877 under the control of Scer\GAL4^insc-Mz1407 (in combination with Dicer-2 expression, for efficient RNAi) leads to a significant increase in Kenyon cell size and to a significant increase in clone cell number, despite of no changes in apoptosis or in neuroblast number and size, within Kenyon cell clusters, as compared to controls.

(Rohith and Shyamala, 2017)

Expression of sd^KK108877 under the control of Scer\GAL4^C587 has no apparent germarium phenotype.

(Li et al., 2015)

Expression of sd^KK108877 under the control of Scer\GAL4^Bx-MS1096 results in male flies with almost complete wing loss.

Expression of sd^KK108877 under the control of Scer\GAL4^Bx-MS1096 results in female flies with almost complete wing loss.

(Schertel et al., 2015)

Scer\GAL4^tj-NP1624-mediated expression results in an incomplete epithelial monolayer of egg chambers at stage 2.

(Berns et al., 2014)

Expression of sd^KK108877 (co-expressed with Dcr-2^Scer\UAS.cDa) under the control of Scer\GAL4^repo.PU reduces glial cell numbers in the third instar larval eye disc.

(Reddy and Irvine, 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^rn.PU/sd^KK108877 is a suppressor | partially of abnormal developmental rate phenotype of Scer\GAL4^rn.PU, Syx7^KK101990

(Boone et al., 2016)

sd^KK108877, Scer\GAL4^GMR.PF is a suppressor of visible phenotype of RtGEF^HMS00741, Scer\GAL4^GMR.PF, yki^{S168A.UAS.YFP}

(Dent et al., 2015)

Scer\GAL4^C587/sd^KK108877 is a suppressor of female sterile phenotype of Scer\GAL4^C587, yki^{RNAi.UASp.cLa}

(Li et al., 2015)

Scer\GAL4^C587/sd^KK108877 is a suppressor of neoplasia | female | adult stage phenotype of Scer\GAL4^C587, yki^{RNAi.UASp.cLa}

(Li et al., 2015)

Scer\GAL4^C587/sd^KK108877 is a suppressor | partially of neoplasia | female | adult stage phenotype of Scer\GAL4^C587, ci^{RNAi.UASp.cLa}

(Li et al., 2015)

sd^KK108877, Scer\GAL4^ptc-559.1 is a suppressor of hyperplasia phenotype of N^UAS.icd, Scer\GAL4^ptc-559.1

(Djiane et al., 2013)

Scer\GAL4^repo.PU/sd^KK108877 is a suppressor of increased cell number | third instar larval stage phenotype of Scer\GAL4^repo.PU, yki^{S168A.UAS.Tag:V5}

(Reddy and Irvine, 2011)

Other

Statement

Reference

Scer\GAL4^Tub.PU, sd^KK108877, wts^x1 has cell lethal | somatic clone | adult stage phenotype

(Xu et al., 2018)

Phenotype Manifest In

Suppressor of

Statement

Reference

sd^KK108877, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of RtGEF^HMS00741, Scer\GAL4^GMR.PF, yki^{S168A.UAS.YFP}

(Dent et al., 2015)

Scer\GAL4^C587/sd^KK108877 is a suppressor of germarium phenotype of Scer\GAL4^C587, yki^{RNAi.UASp.cLa}

(Li et al., 2015)

Scer\GAL4^C587/sd^KK108877 is a suppressor | partially of germarium phenotype of Scer\GAL4^C587, ci^{RNAi.UASp.cLa}

(Li et al., 2015)

Scer\GAL4^repo.PU/sd^KK108877 is a suppressor of embryonic/larval glial cell | third instar larval stage phenotype of Scer\GAL4^repo.PU, yki^{S168A.UAS.Tag:V5}

(Reddy and Irvine, 2011)

Scer\GAL4^repo.PU/sd^KK108877 is a suppressor of eye disc | third instar larval stage phenotype of Scer\GAL4^repo.PU, yki^{S168A.UAS.Tag:V5}

(Reddy and Irvine, 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Clones that are both wts^x1 homozygous and express sd^KK108877 under the control of Scer\GAL4^Tub.PU are lethal in the wing.

(Xu et al., 2018)

The marked delay in pupariation characteristic for larvae expressing Syx7^KK101990 under the control of Scer\GAL4^rn.PU can be partially suppressed by co-expression of sd^KK108877.

(Boone et al., 2016)

sd^KK108877 completely suppresses the eye overgrowth phenotype seen in flies co-expressing RtGEF^HMS00741 and yki^{S168A.Scer\UAS.T:Avic\GFP-YFP} under the control of Scer\GAL4^GMR.PF.

(Dent et al., 2015)

The female sterility due to tumorous germaria induced by Scer\GAL4^C587-driven expression of yki^{dsRNA.shRNA.Scer\UAS.P\T} is rescued by co-expression of sd^KK108877 - the double knock-down females are fertile and without significant germaria defects.

The rather moderate tumorous germarium phenotype characteristic for adult female flies expressing ci^{dsRNA.shRNA.Scer\UAS.P\T} under the control of Scer\GAL4^C587 is significantly improved by co-expression of sd^KK108877.

(Li et al., 2015)

Expression of sd^KK108877 suppresses the increase in glial cell number seen in larval eye discs expressing yki^{S168A.Scer\UAS.T:SV5\V5} under the control of Scer\GAL4^repo.PU.

(Reddy and Irvine, 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v101497

P{KK108877}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

sd^KK108877

Name Synonyms

Secondary FlyBase IDs

References (19)

Report Sections

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