Simultaneous expression of dsb^GD2254 and dsb^KK107147 under the control of either Scer\GAL4^MJ12a or Scer\GAL4^grh.D4 leads to dorsal trunk elongation defects and internalization of the posterior spiracles into the body cavity. Most larvae survive until third instar larval stage and show a moderate growth reduction. Scer\GAL4^btl.PS-driven expression does not result in any visible phenotype. Individual expression of dsb^KK107147 controlled by Scer\GAL4^grh.D4 also results in impaired dorsal trunk elongation and in partial lethality.

Expression of dsb^KK107147 RNAi under the control of Scer\GAL4^pnr-MD237 does not change the level of pigmentation on the abdominal tergite 5 or 6 but expression under the control of Scer\GAL4^Ubi.PU results in significantly decreased pigmentation on the abdominal tergite 5 in adult females compared to controls.