UASt regulatory sequences drive expression of an inverted repeat.
Expressing Cul4KK101901 under the control of Scer\GAL4insc-Mz1407, but not Scer\GAL4repo.PU, leads to larval brain neuroblasts showing signs of quiescence: neuroblasts show a significant decrease in cell divisions (both DNA replication/EdU staining and Mitosis/phospho-H3); and frequently show a primary cellular process, whereas control neuroblasts show a typically rounded morphology. (Experiments were done in combination with Dicer-2 for higher RNAi efficiency).
Expression under the control of Scer\GAL4Mef2.PR results in sarcomeres with irregular aggregates of Z-lines in the larval body wall muscles.
Cul4KK101901, Scer\GAL4insc-Mz1407 has abnormal cell shape | larval stage phenotype, suppressible by wtsHMS00026, Scer\GAL4insc-Mz1407
Cul4KK101901, Scer\GAL4insc-Mz1407 has decreased occurrence of cell division | larval stage phenotype, suppressible | partially by wtsHMS00026, Scer\GAL4insc-Mz1407
Cul4KK101901, Scer\GAL4insc-Mz1407 has abnormal neuroanatomy | larval stage phenotype, suppressible | partially by wtsHMS00026, Scer\GAL4insc-Mz1407
Cul4KK101901, Scer\GAL4insc-Mz1407 has abnormal cell shape | larval stage phenotype, suppressible by ykiS168A.UAS.Tag:V5, Scer\GAL4insc-Mz1407
Cul4KK101901, Scer\GAL4insc-Mz1407 has decreased occurrence of cell division | larval stage phenotype, suppressible | partially by ykiS168A.UAS.Tag:V5, Scer\GAL4insc-Mz1407
Cul4KK101901, Scer\GAL4insc-Mz1407 has abnormal neuroanatomy | larval stage phenotype, suppressible | partially by ykiS168A.UAS.Tag:V5, Scer\GAL4insc-Mz1407
Cul4KK101901, Scer\GAL4unspecified, ninaEP37H.GMR has visible | adult stage phenotype
Cul4KK101901, Scer\GAL4insc-Mz1407 has larval neuroblast | larval stage phenotype, suppressible | partially by wtsHMS00026, Scer\GAL4insc-Mz1407
Cul4KK101901, Scer\GAL4insc-Mz1407 has embryonic/larval brain | larval stage phenotype, suppressible | partially by wtsHMS00026, Scer\GAL4insc-Mz1407
Cul4KK101901, Scer\GAL4insc-Mz1407 has larval neuroblast | larval stage phenotype, suppressible | partially by ykiS168A.UAS.Tag:V5, Scer\GAL4insc-Mz1407
Cul4KK101901, Scer\GAL4insc-Mz1407 has embryonic/larval brain | larval stage phenotype, suppressible | partially by ykiS168A.UAS.Tag:V5, Scer\GAL4insc-Mz1407
Cul4KK101901, Scer\GAL4unspecified, ninaEP37H.GMR has eye phenotype