FB2024_03 , released June 25, 2024
Allele: Dmel\kuneC309
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General Information
Symbol
Dmel\kuneC309
Species
D. melanogaster
Name
FlyBase ID
FBal0222440
Feature type
allele
Associated gene
Dmel\kune
Associated Insertion(s)
PBac{3HPy+}kuneC309
Carried in Construct
Key Links
Genomic Maps

Allele class

amorphic allele - genetic evidence

Mutagen
    Nature of the Allele
    Allele class

    amorphic allele - genetic evidence

    (Nelson et al., 2010)
    Mutagen
    Progenitor genotype
    Associated Insertion(s)
    PBac{3HPy+}kuneC309
    Cytology
    Description

    Insertion in the 5' untranslated region.

    (Nelson et al., 2010)
    Mutations Mapped to the Genome
    Curation Data
    Variant Molecular Consequences
    Associated Sequence Data
    DDBJ / EMBL / GenBank
    DNA sequence
    Protein sequence
     
    UniProtKB/Swiss-Prot
      UniProtKB/TrEMBL
        Expression Data
        Reporter Expression
        Additional Information
        Statement
        Reference
         
        Marker for
        Reflects expression of
        Reporter construct used in assay
        Human Disease Associations
        Disease Ontology (DO) Annotations
        Models Based on Experimental Evidence ( 0 )
        Disease
        Evidence
        References
        Modifiers Based on Experimental Evidence ( 0 )
        Disease
        Interaction
        References
        Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
         
        Disease-implicated variant(s)
         
        Phenotypic Data
        Phenotypic Class
        Phenotype Manifest In
        Detailed Description
        Statement
        Reference

        A fluorescent 10kD dye injected into the body cavity readily leaks into the lumens of the trachea and salivary gland in mutant embryos, indicating a loss of paracellular barrier function. In addition, the fluorescent 10kD dye penetrates into the nerve cord of mutant embryos, indicating a defect in the blood-brain barrier.

        The length of the dorsal trunk of stage 16 homozygous and kuneC309/Df(2R)BSC696 embryos is significantly increased compared to controls, and the mutant dorsal trunks appear tortuous. Mutant trachea have a diffuse, disorganized lumenal matrix (in contrast to wild-type trachea which contain a dense, fibrillar chitin cable) and lack the gap between the chitin cable and the apical surface of the cells that is seen in wild-type trachea.

        (Nelson et al., 2010)
        External Data
        Interactions
        Show genetic interaction network for Enhancers & Suppressors
        Phenotypic Class
        Phenotype Manifest In
        Additional Comments
        Genetic Interactions
        Statement
        Reference

        The tracheal length defects of kuneC309 pckG0012 sinunwu7 triple mutant embryos are no more severe than those of single mutants.

        (Nelson et al., 2010)
        Xenogenetic Interactions
        Statement
        Reference
        Complementation and Rescue Data
        Rescued by

        kuneC309 is rescued by kuneUAS.cNa/Scer\GAL4da.G32

        (Nelson et al., 2010)
        Comments

        At 23[o]C, expression of kuneScer\UAS.cNa under the control of Scer\GAL4da.G32 only partially rescues the septate junction defects of kuneC309 mutants and does not rescue their embryonic lethality. At 28[o]C, expression of kuneScer\UAS.cNa under the control of Scer\GAL4da.G32 rescues both the septate junction defects and embryonic lethality of kuneC309 mutants.

        (Nelson et al., 2010)
        Images (0)
        Mutant
        Wild-type
        Stocks (1)
        Notes on Origin
        Discoverer
        External Crossreferences and Linkouts ( 0 )
        Synonyms and Secondary IDs (3)
        Reported As
        Symbol Synonym
        CG1298C309
        (Gambetta and Müller, 2009.8.18)
        kuneC309
        (Luedke et al., 2024, Khadilkar et al., 2017, Deligiannaki et al., 2015, Nelson et al., 2010)
        kunec309
        (Tempesta et al., 2017, Hall et al., 2014)
        Name Synonyms
        Secondary FlyBase IDs
          References (8)