Amino acid replacement: T16N.
RagA-BT16N.Scer\UAS-expressing clones (generated using MARCM and Scer\GAL4ppk.PG) show relatively mild dendrite growth defects in the large C4da dendritic arbors.
Ectopic expression of RagA-BT16N.Scer\UAS under the control of the Scer\GAL4elav-C155 results in a decrease in the number of neuromuscular junction boutons in third instar larvae.
Expression of RagAT16N.Scer\UAS using Scer\GAL4nub-AC-62 results in abnormally small wings in which cell size and cell number are decreased.
Expression of RagAT16N.Scer\UAS under the control of Scer\GAL4en-e16E results in a significant decrease in the size of the posterior compartment of the wing, compared with that of the control anterior compartment of the wing. The average size of individual cells in the posterior compartment of the wing is significantly decreased in these flies.
Under normal fed conditions, expression of RagAT16N.Scer\UAS under the control of Scer\GAL4unspecified in clones in the larval fat body results in a potent decrease in cell size compared with neighbouring control cells. Under starvation conditions, expression of RagAT16N.Scer\UAS under the control of Scer\GAL4unspecified in clones in the larval fat body has no effect on cell size compared to controls.
RagA-BT16N.UAS, Scer\GAL4nub-AC-62 has abnormal size phenotype, enhanceable by hppyUAS.cMa, Scer\GAL4nub-AC-62
RagA-BT16N.UAS, Scer\GAL4nub-AC-62 has decreased cell number phenotype, enhanceable by hppyUAS.cMa, Scer\GAL4nub-AC-62
RagA-BT16N.UAS, Scer\GAL4nub-AC-62 has decreased cell size phenotype, enhanceable by hppyUAS.cMa, Scer\GAL4nub-AC-62
RagA-BT16N.UAS/Scer\GAL4pHCl-2.PR is a non-enhancer of abnormal developmental rate | recessive phenotype of pHCl-2pTV-Cherry
Scer\GAL4elav-C155/RagA-BT16N.UAS is a non-enhancer of abnormal neuroanatomy | third instar larval stage phenotype of Trpml1
Scer\GAL4VGlut-OK371/RagA-BT16N.UAS is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of hiwND8
RagA-BT16N.UAS/Scer\GAL4pHCl-2.PR is a non-suppressor of abnormal developmental rate | recessive phenotype of pHCl-2pTV-Cherry
RagA-BT16N.UAS, Scer\GAL4nub-AC-62 has wing phenotype, enhanceable by hppyUAS.cMa, Scer\GAL4nub-AC-62
Scer\GAL4elav-C155/RagA-BT16N.UAS is a non-enhancer of neuromuscular junction | third instar larval stage phenotype of Trpml1
Scer\GAL4elav-C155/RagA-BT16N.UAS is a non-enhancer of NMJ bouton | third instar larval stage phenotype of Trpml1
Scer\GAL4VGlut-OK371/RagA-BT16N.UAS is a suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of hiwND8
Scer\GAL4VGlut-OK371/RagA-BT16N.UAS is a suppressor of NMJ bouton | third instar larval stage phenotype of hiwND8
Ectopic expression of RagA-BT16N.Scer\UAS under the control of the Scer\GAL4elav-C155 driver does not cause further decrease in the number of neuromuscular junction (NMJ) boutons in the already NMJ boutons-deficient Trpml1 mutant third instar larvae.
Ectopic expression of RagA-BT16N.Scer\UAS under the control of the Scer\GAL4VGlut-OK371 driver in hiwND8 mutant larvae background rescues the increased number of neuromuscular junction boutons phenotype characteristic for hiwND8 mutants.
Expression of RagAT16N.Scer\UAS under the control of Scer\GAL4Cg.PA does not enhance the increase in Lysotracker-positive vesicle volume seen in trpml1 mutant fat body cells.
Co-expression of hppyScer\UAS.cMa enhances the decrease in wing size, wing cell number and wing cell size resulting from expression of RagA[T16N.Scer\UAS] using Scer\GAL4nub-AC-62.