FB2024_03 , released June 25, 2024
Allele: Dmel\nanos+1.18xTag:MS2
Open Close
General Information
Symbol
Dmel\nanos+1.18xTag:MS2
Species
D. melanogaster
Name
FlyBase ID
FBal0221309
Feature type
allele
Associated gene
Dmel\nanos
Associated Insertion(s)
Carried in Construct
P{nanos+1-(ms2).18}
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Brechbiel and Gavis, 2008)
Progenitor genotype
nanosgnanosb
(Brechbiel and Gavis, 2008)
Carried in construct
P{nanos+1-(ms2).18}
Cytology
Description

A 450bp HpaI-XmnI fragment from the nos 3'UTR sequence has been deleted (this removes three of the four localisation elements present within the 3'UTR). A 1.1kb fragment containing 18 tandemly repeated Tag:MS2 stem loops has been inserted into the BclI site of the nos +6 element.

(Brechbiel and Gavis, 2008)
Allele components
Component
Use(s)
Regulatory region(s)
nanos
Encoded product / tool
nanos
Tagged with
Tag:MS2
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      third instar larval stage
      dendritic arborizing neuron
      (Brechbiel and Gavis, 2008)
      Additional Information
      Statement
      Reference

      nos+1.18.T:MS2MCP.BS transcript is localized to the dendritic processes of DA neurons.

      (Brechbiel and Gavis, 2008)
       
      Marker for
      Reflects expression of
      nanos
      (Brechbiel and Gavis, 2008)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to rescue

      nanos+1.18xTag:MS2 fails to rescue nanos53/nanos18

      (Brechbiel and Gavis, 2008)
      Comments

      The reduction in dendritic branching of the class IV da neurons that is seen in late third instar nos18/nos53 larvae is not rescued by nos+2.18.T:MS2\MCP.BS.

      (Brechbiel and Gavis, 2008)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      nanos+1.18xTag:MS2
      nos+1.18.T:MS2\MCP.BS
      nos+1.18.Tag:BS(MS2-CP)
      nos+1.18xTag:MS2
      Name Synonyms
      Secondary FlyBase IDs
        References (2)