FB2024_03 , released June 25, 2024
Allele: Dmel\PsnCTF.αTub84B.Tag:HA
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General Information
Symbol
Dmel\PsnCTF.αTub84B.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0221188
Feature type
allele
Associated gene
Dmel\Psn
Associated Insertion(s)
Carried in Construct
P{tub-Psn.CTF}
Key Links
Transgenic product class
inframe_deletion
(Barakat et al., 2009)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(Barakat et al., 2009)
Mutagen
in vitro construct
(Barakat et al., 2009)
Progenitor genotype
Carried in construct
P{tub-Psn.CTF}
Cytology
Description

The αTub84B promoter drives expression of the C-terminal half of Psn (most of the amino acids N terminal to Val314 of Psn are truncated) which is tagged with two copies of the Tag:HA tag inserted between the Gly361 and Gln362 residues.

(Barakat et al., 2009)
Allele components
Component
Use(s)
Regulatory region(s)
αTub84B
Encoded product / tool
Psn
Tagged with
Tag:HA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Flies expressing PsnCTF.αTub84B.T:Ivir\HA1 are visibly normal.

      Flies co-expressing both PsnNTF.αTub84B.T:Hsap\MYC and PsnCTF.αTub84B.T:Ivir\HA1 are visibly normal.

      (Barakat et al., 2009)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      PsnNTF.αTub84B.Tag:MYC/PsnCTF.αTub84B.Tag:HA rescues PsnC2

      (Barakat et al., 2009)
      Fails to rescue

      PsnCTF.αTub84B.Tag:HA fails to rescue PsnC2

      (Barakat et al., 2009)
      Comments

      Wings containing clones of homozygous PsnC2 cells in flies expressing only PsnCTF.αTub84B.T:Ivir\HA1 still show a characteristic wing notching, indicating a failure of rescue by PsnCTF.αTub84B.T:Ivir\HA1 alone.

      PsnC2 flies that co-express both PsnNTF.αTub84B.T:Hsap\MYC and PsnCTF.αTub84B.T:Ivir\HA1 are viable and fertile and have no visible developmental defects.

      (Barakat et al., 2009)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      PsnCTF.αTub84B.T:Ivir\HA1
      PsnCTF.αTub84B.Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (1)