Nucleotide substitution: C1718T.
Amino acid replacement: Q483term.
C5604944T
C1718T
Q483term | asl-PA
Q483term
cilium & mechanosensory sensory organ
microtubule basal body & mechanosensory sensory organ
microtubule basal body & spermatid
aslmecD homozygous third instar larval brains do not display obvious changes in volume, as compared to controls.
The 'remnant' centrioles (originally built from maternally deposited protein and inherited by the developing tissues, asl protein-negative) present in the testes of aslmecD homozygous mutant third instar larvae are not evenly distributed as centrioles in control flies but are located in four distinct cell populations: hub cells, male germline stem cells, proximal and distal germline cells.
aslmecD homozygotes display abnormally long remnant centrioles in the male germline stem cells (but not in the adjacent hub cells) that grow progressively bigger as development proceeds (larvae 72 and 96 after egg laying, pharate adults). The centrioles are being lost from male germline stem cells over time. Remnant centrioles in the female germline stem cells in aslmecD homozygous pharate adults or in the larval wing disc cells are also longer compared to controls.
aslmecD/Df(3R)ED5177 male third instar larvae also display abnormally long remnant centrioles in their germline stem cells compared to controls.
Remnant centrioles in aslmecD male homozygote pupae or pharate adults display defects in nuclear attachment and/or axoneme formation and the nuclei of late stage spermatids are fragmented and dispersed.
Mutant flies cannot stand on their legs, which are crossed, and their wings extend upwards.
The basal body and sensory cilium are missing in the mechanosensory neurons of mutant flies.
Spermatid tails are still formed in mutant males, but contain abnormal mitochondrial derivatives and the axonemes are completely absent. No basal bodies are found in the mutant spermatid cysts.
"Maternally contributed" (MC) centrioles containing wild-type asl protein from the mother are stable in aslmecD testes, being present in stem cells at the tip of the testis, and remaining in the stem cells until late pupal stages. However, no daughter centrioles are found near these MC centrioles, in contrast to wild-type testes, where the mother centrioles are often associated with daughter centriole. The MC centrioles elongate in spermatocytes, as occurs in wild type and the recruitment of pericentriolar material by the MC centrioles is not impaired.
aslmecD, mad2G6595 has abnormal neuroanatomy | third instar larval stage phenotype
aslmecD, mad2G6595 has abnormal size | third instar larval stage phenotype
aslmecD has centriole | progressive phenotype, suppressible | partially by Cep97Ubi-p63E.EGFP,Tag:PACT(Plp)
aslmecD has male germline stem cell | progressive phenotype, suppressible | partially by Cep97Ubi-p63E.EGFP,Tag:PACT(Plp)
aslmecD has centriole | progressive phenotype, non-suppressible by Cep97Ubi-p63E.EGFP
aslmecD has male germline stem cell | progressive phenotype, non-suppressible by Cep97Ubi-p63E.EGFP
aslmecD has centriole | progressive phenotype, non-suppressible by PlpPACT.Ubi-p63E.EGFP
aslmecD has male germline stem cell | progressive phenotype, non-suppressible by PlpPACT.Ubi-p63E.EGFP
aslmecD, mad2G6595 has embryonic/larval brain | third instar larval stage phenotype
The remnant centrioles growing progressively longer as development proceeds (from larvae 72 and 96 hr after egg laying to pharate adults) phenotype observed in male germline stem cells of aslmecD homozygotes can be partially suppressed by expression of Cep97Ubi-p63E.T:Avic\GFP-EGFP,T:PACT-Plp but not that of either Cep97Ubi-p63E.T:Avic\GFP-EGFP or PlpPACT.Ubi-p63E.T:Avic\GFP-EGFP alone.
aslmecD is rescued by aslUAS.asl.GFP